################################## # NO CUT ENZYMES ( 182 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 23 enzymes) # ################################### BanI 235 GGCACC G^G[CT][GA]CC BfaI 301 CTAG C^TAG BsgI 421 GTGCAG GTGCAG(16/14) BsrBI 35 CCGCTC CCGCTC(-3/-3) BstXI 239 CCACCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EcoNI 190 CCTGTGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 158 CCATGG C^C[AT][AT]GG HapII 147 CCGG C^CGG HinfI 150 GACTC G^A[ACGT]TC HpaII 147 CCGG C^CGG MaeI 301 CTAG C^TAG MaeIII 89 GTGAC ^GT[ACGT]AC MseI 332 TTAA T^TAA MslI 240 CACCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 147 CCGG C^CGG NcoI 158 CCATGG C^CATGG NlaIII 159 CATG CATG^ PleI 150 GACTC GAGTC(4/5) PstI 127 CTGCAG CTGCA^G SexAI 440 ACCTGGT A^CC[AT]GGT StyI 158 CCATGG C^C[AT][AT]GG Tru9I 332 TTAA T^TAA Tsp45I 89 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AfaI 52 GTAC GT^AC 207 GTAC AluI 66 AGCT AG^CT 107 AGCT AlwI 219 GGATC GGATC(4/5) 273 GGATC AlwNI 55 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG 103 CAGCAGCTG ApaI 26 GGGCCC GGGCC^C 186 GGGCCC BanII 26 GGGCCC G[GA]GC[CT]^C 186 GGGCCC BbvI 105 GCAGC GCAGC(8/12) 305 GCTGC BsiYI 190 CCTGTGGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 316 CCACTGGTAGG BslI 190 CCTGTGGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 316 CCACTGGTAGG BsrI 318 ACTGG ACTGG(1/-1) 356 ACTGG BstF5I 228 CATCC GGATG(2/0) 249 GGATG DpnII 220 GATC ^GATC 274 GATC DrdI 63 GACAGCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 156 GACCATGGTGTC DsaI 43 CCGTGG C^C[GA][CT]GG 158 CCATGG EaeI 32 TGGCCG [CT]^GGCC[GA] 464 TGGCCG FokI 228 CATCC GGATG(9/13) 249 GGATG MboI 220 GATC ^GATC 274 GATC MboII 258 GAAGA GAAGA(8/7) 261 GAAGA NdeII 220 GATC ^GATC 274 GATC PpuMI 56 AGGTCCT [GA]G^G[AT]CC[CT] 70 GGGTCCC PvuII 65 CAGCTG CAG^CTG 106 CAGCTG RsaI 52 GTAC GT^AC 207 GTAC Sau3AI 220 GATC ^GATC 274 GATC SfaNI 250 GATGC GCATC(5/9) 385 GATGC SfcI 127 CTGCAG C^T[GA][CT]AG 367 CTACAG TseI 105 GCAGC G^C[AT]GC 305 GCTGC TspRI 254 CACTG CA[GC]TG[ACGT][ACGT]^ 317 CACTG XcmI 158 CCATGGTGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 431 CCAACTTCTACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AciI AvaII BmyI BsaJI BsmFI Bsp1286I BssKI BstNI Cac8I Cfr13I DraII EcoO109I EcoRII FauI Fnu4HI HaeIII HphI ItaI MnlI MspA1I MvaI MwoI NlaIV Sau96I ScrFI