Restriction map for
pFN21AE6125
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# NO CUT ENZYMES ( 182 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DdeI DraI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tsp509I Tth111I TthHB8I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 23 enzymes) #
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BanI 235 GGCACC G^G[CT][GA]CC
BfaI 301 CTAG C^TAG
BsgI 421 GTGCAG GTGCAG(16/14)
BsrBI 35 CCGCTC CCGCTC(-3/-3)
BstXI 239 CCACCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoNI 190 CCTGTGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 158 CCATGG C^C[AT][AT]GG
HapII 147 CCGG C^CGG
HinfI 150 GACTC G^A[ACGT]TC
HpaII 147 CCGG C^CGG
MaeI 301 CTAG C^TAG
MaeIII 89 GTGAC ^GT[ACGT]AC
MseI 332 TTAA T^TAA
MslI 240 CACCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 147 CCGG C^CGG
NcoI 158 CCATGG C^CATGG
NlaIII 159 CATG CATG^
PleI 150 GACTC GAGTC(4/5)
PstI 127 CTGCAG CTGCA^G
SexAI 440 ACCTGGT A^CC[AT]GGT
StyI 158 CCATGG C^C[AT][AT]GG
Tru9I 332 TTAA T^TAA
Tsp45I 89 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 52 GTAC GT^AC
207 GTAC
AluI 66 AGCT AG^CT
107 AGCT
AlwI 219 GGATC GGATC(4/5)
273 GGATC
AlwNI 55 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
103 CAGCAGCTG
ApaI 26 GGGCCC GGGCC^C
186 GGGCCC
BanII 26 GGGCCC G[GA]GC[CT]^C
186 GGGCCC
BbvI 105 GCAGC GCAGC(8/12)
305 GCTGC
BsiYI 190 CCTGTGGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
316 CCACTGGTAGG
BslI 190 CCTGTGGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
316 CCACTGGTAGG
BsrI 318 ACTGG ACTGG(1/-1)
356 ACTGG
BstF5I 228 CATCC GGATG(2/0)
249 GGATG
DpnII 220 GATC ^GATC
274 GATC
DrdI 63 GACAGCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
156 GACCATGGTGTC
DsaI 43 CCGTGG C^C[GA][CT]GG
158 CCATGG
EaeI 32 TGGCCG [CT]^GGCC[GA]
464 TGGCCG
FokI 228 CATCC GGATG(9/13)
249 GGATG
MboI 220 GATC ^GATC
274 GATC
MboII 258 GAAGA GAAGA(8/7)
261 GAAGA
NdeII 220 GATC ^GATC
274 GATC
PpuMI 56 AGGTCCT [GA]G^G[AT]CC[CT]
70 GGGTCCC
PvuII 65 CAGCTG CAG^CTG
106 CAGCTG
RsaI 52 GTAC GT^AC
207 GTAC
Sau3AI 220 GATC ^GATC
274 GATC
SfaNI 250 GATGC GCATC(5/9)
385 GATGC
SfcI 127 CTGCAG C^T[GA][CT]AG
367 CTACAG
TseI 105 GCAGC G^C[AT]GC
305 GCTGC
TspRI 254 CACTG CA[GC]TG[ACGT][ACGT]^
317 CACTG
XcmI 158 CCATGGTGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
431 CCAACTTCTACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI AvaII BmyI BsaJI BsmFI
Bsp1286I BssKI BstNI Cac8I Cfr13I
DraII EcoO109I EcoRII FauI Fnu4HI
HaeIII HphI ItaI MnlI MspA1I
MvaI MwoI NlaIV Sau96I ScrFI