Restriction map for
pFN21AE6127
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI AlwNI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI HaeII HapII Hin1I
HincII HindII HindIII HinfI HpaI
HpaII KasI KpnI Ksp632I KspI
MaeI MaeIII MamI MfeI MflI
MluI MluNI MroI MscI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tsp45I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 400 GTCTAC GT^[AC][GT]AC
AciI 376 GCGG CCGC(-3/-1)
AluI 296 AGCT AG^CT
Alw44I 390 GTGCAC G^TGCAC
AlwI 360 GATCC GGATC(4/5)
ApaI 312 GGGCCC GGGCC^C
ApaLI 390 GTGCAC G^TGCAC
AspHI 390 GTGCAC G[AT]GC[AT]^C
AvaII 329 GGACC G^G[AT]CC
AviII 176 TGCGCA TGC^GCA
BanII 312 GGGCCC G[GA]GC[CT]^C
BbvCI 84 GCTGAGG CCTCAGC(-5/-2)
BsaJI 395 CCAGGG C^C[ACGT][ACGT]GG
BsiEI 34 CGATCG CG[GA][CT]^CG
BsiHKAI 390 GTGCAC G[AT]GC[AT]^C
BsiYI 344 CCAACGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 344 CCAACGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 143 GAGAC GTCTC(1/5)
BsrI 20 ACTGG ACTGG(1/-1)
BssSI 324 CTCGTG CACGAG(-5/-1)
Cac8I 117 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 177 GCGC GCG^C
DdeI 85 CTGAG C^T[ACGT]AG
FspI 176 TGCGCA TGC^GCA
HgaI 109 GCGTC GACGC(5/10)
HhaI 177 GCGC GCG^C
HinP1I 177 GCGC G^CGC
MboII 90 GAAGA GAAGA(8/7)
MseI 191 TTAA T^TAA
MslI 168 CACCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII 369 CATG CATG^
PstI 104 CTGCAG CTGCA^G
PvuI 34 CGATCG CGAT^CG
SfaNI 81 GATGC GCATC(5/9)
SfcI 104 CTGCAG C^T[GA][CT]AG
TaqI 47 TCGA T^CGA
Tru9I 191 TTAA T^TAA
Tsp509I 53 AATT ^AATT
TspRI 122 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 47 TCGA T^CGA
XcmI 113 CCAAGCCAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BbvI 106 GCAGC GCAGC(8/12)
103 GCTGC
BmyI 312 GGGCCC G[AGT]GC[ACT]^C
390 GTGCAC
BseRI 208 GAGGAG GAGGAG(10/8)
217 GAGGAG
Bsp1286I 312 GGGCCC G[AGT]GC[ACT]^C
390 GTGCAC
BssKI 252 CCTGG ^CC[ACGT]GG
395 CCAGG
BstNI 252 CCTGG CC^[AT]GG
395 CCAGG
EcoRII 252 CCTGG ^CC[AT]GG
395 CCAGG
Fnu4HI 103 GCTGC GC^[ACGT]GC
106 GCAGC
HaeIII 239 GGCC GG^CC
313 GGCC
HphI 72 GGTGA GGTGA(8/7)
255 GGTGA
ItaI 103 GCTGC GC^[ACGT]GC
106 GCAGC
MaeII 383 ACGT A^CGT
404 ACGT
MvaI 252 CCTGG CC^[AT]GG
395 CCAGG
MwoI 57 GCTCAAAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
97 GCTGTGGCTGC
NlaIV 312 GGGCCC GG[ACGT]^[ACGT]CC
313 GGCCCC
ScrFI 252 CCTGG CC^[ACGT]GG
395 CCAGG
TseI 103 GCTGC G^C[AT]GC
106 GCAGC
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
BstF5I Cfr13I DpnII DraII EcoO109I
FokI MboI MnlI NdeII Sau3AI
Sau96I