Restriction map for
pFN21AE1433
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BcgI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstZ17I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MroI MseI MunI NarI
NdeI NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcyI 315 GACGCC G[GA]^CG[CT]C
AocI 1398 CCTGAGG CC^T[ACGT]AGG
ApaI 950 GGGCCC GGGCC^C
BbsI 486 GAAGAC GAAGAC(2/6)
BbvCI 742 GCTGAGG CCTCAGC(-5/-2)
BciVI 521 GTATCC GTATCC(6/5)
BpuAI 486 GAAGAC GAAGAC(2/6)
BsaHI 315 GACGCC G[GA]^CG[CT]C
BsmFI 1091 GTCCC GGGAC(10/14)
BsrBI 1444 GAGCGG CCGCTC(-3/-3)
BsrFI 154 GCCGGC [GA]^CCGG[CT]
BssHII 76 GCGCGC G^CGCGC
BstBI 782 TTCGAA TT^CGAA
BstEII 226 GGTGACC G^GT[ACGT]ACC
BstPI 226 GGTGACC G^GT[ACGT]ACC
BstXI 1103 CCAATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 386 AGATCC [GA]^GATC[CT]
Bsu36I 1398 CCTGAGG CC^T[ACGT]AGG
Cfr10I 154 GCCGGC [GA]^CCGG[CT]
Eco81I 1398 CCTGAGG CC^T[ACGT]AGG
EcoNI 282 CCTGAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 226 GGTGACC G^GT[ACGT]ACC
HgaI 315 GACGC GACGC(5/10)
Hin1I 315 GACGCC G[GA]^CG[CT]C
MaeII 855 ACGT A^CGT
MboII 486 GAAGA GAAGA(8/7)
MflI 386 AGATCC [GA]^GATC[CT]
MspA1I 43 CAGCTG C[AC]G^C[GT]G
NaeI 154 GCCGGC GCC^GGC
NgoMIV 154 GCCGGC G^CCGGC
NspV 782 TTCGAA TT^CGAA
PflMI 1211 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1334 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 43 CAGCTG CAG^CTG
SacI 1237 GAGCTC GAGCT^C
ScaI 447 AGTACT AGT^ACT
SfuI 782 TTCGAA TT^CGAA
SmaI 1331 CCCGGG CCC^GGG
Van91I 1211 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 386 AGATCC [GA]^GATC[CT]
XmaCI 1331 CCCGGG C^CCGGG
XmaI 1331 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaI 806 CCCGAG C^[CT]CG[GA]G
1331 CCCGGG
AvaII 1335 GGTCC G^G[AT]CC
1425 GGACC
BanI 730 GGCACC G^G[CT][GA]CC
1126 GGCACC
BglI 1300 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1366 GCCACAGTGGC
BpmI 477 CTCCAG CTGGAG(16/14)
1019 CTGGAG
BsaI 1393 GAGACC GGTCTC(1/5)
1220 GGTCTC
BsmAI 1393 GAGAC GTCTC(1/5)
1221 GTCTC
BsoBI 806 CCCGAG C^[CT]CG[GA]G
1331 CCCGGG
BspMI 26 ACCTGC ACCTGC(4/8)
490 ACCTGC
DraIII 898 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
911 CACTGAGTG
HaeII 151 AGCGCC [GA]GCGC^[CT]
1409 GGCGCT
HinfI 162 GACTC G^A[ACGT]TC
1341 GAGTC
NcoI 1251 CCATGG C^CATGG
1295 CCATGG
PleI 162 GACTC GAGTC(4/5)
1341 GAGTC
PstI 649 CTGCAG CTGCA^G
1042 CTGCAG
XcmI 927 CCAGGAAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1295 CCATGGCCAAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI BalI BanII BbvI BcnI
BfaI BmyI BsaJI BsgI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeI MaeIII MboI MluNI MnlI
MscI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI Tsp45I
TspRI TthHB8I