Restriction map for
pFN21AE6122
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# NO CUT ENZYMES ( 203 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AluI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AvaII AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BbvI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DdeI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HaeIII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaI HphI KasI
Ksp632I KspI MamI MfeI MflI
MluI MluNI MroI MscI MseI
MslI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI TfiI Tru9I TseI Tsp509I
TspRI Tth111I TthHB8I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 167 GGTACC G^GTACC
Asp718I 167 GGTACC G^GTACC
AvaI 247 CCCGGG C^[CT]CG[GA]G
BanI 167 GGTACC G^G[CT][GA]CC
BbsI 7 GAAGAC GAAGAC(2/6)
BfaI 80 CTAG C^TAG
BpuAI 7 GAAGAC GAAGAC(2/6)
BsaI 16 GAGACC GGTCTC(1/5)
BsiYI 197 CCACATCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 197 CCACATCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 16 GAGAC GTCTC(1/5)
BsmFI 186 GTCCC GGGAC(10/14)
BsoBI 247 CCCGGG C^[CT]CG[GA]G
BsrDI 127 GCAATG GCAATG(2/0)
Cac8I 89 GCTGGC GC[ACGT]^[ACGT]GC
DpnII 255 GATC ^GATC
EcoRV 148 GATATC GAT^ATC
Fnu4HI 56 GCCGC GC^[ACGT]GC
HapII 248 CCGG C^CGG
HpaII 248 CCGG C^CGG
ItaI 56 GCCGC GC^[ACGT]GC
KpnI 167 GGTACC GGTAC^C
MaeI 80 CTAG C^TAG
MaeII 241 ACGT A^CGT
MaeIII 208 GTCAC ^GT[ACGT]AC
MboI 255 GATC ^GATC
MspA1I 114 CCGCTG C[AC]G^C[GT]G
MspI 248 CCGG C^CGG
MwoI 127 GCAATGACCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 255 GATC ^GATC
NlaIII 12 CATG CATG^
NlaIV 167 GGTACC GG[ACGT]^[ACGT]CC
PflMI 197 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau3AI 255 GATC ^GATC
SmaI 247 CCCGGG CCC^GGG
Tsp45I 208 GTCAC ^GT[GC]AC
Van91I 197 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 247 CCCGGG C^CCGGG
XmaI 247 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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AfaI 168 GTAC GT^AC
180 GTAC
BcnI 247 CCCGG CC^[GC]GG
248 CCGGG
BsaJI 203 CCTGGG C^C[ACGT][ACGT]GG
247 CCCGGG
BsrI 215 ACTGG ACTGG(1/-1)
183 CCAGT
BstF5I 200 CATCC GGATG(2/0)
219 GGATG
BstNI 102 CCTGG CC^[AT]GG
203 CCTGG
EcoRII 102 CCTGG ^CC[AT]GG
203 CCTGG
FokI 200 CATCC GGATG(9/13)
219 GGATG
MboII 7 GAAGA GAAGA(8/7)
252 GAAGA
MnlI 189 CCTC CCTC(7/6)
143 GAGG
MvaI 102 CCTGG CC^[AT]GG
203 CCTGG
NciI 247 CCCGG CC^[GC]GG
248 CCGGG
RsaI 168 GTAC GT^AC
180 GTAC
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# 3 OR MORE CUT ENZYMES ( 3 enzymes) #
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AciI BssKI ScrFI