Restriction map for
pFN21AB8024
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsiYI
BslI BsmAI BsmBI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstF5I BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FauI FbaI FokI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MamI MflI MluI
MluNI MroI MscI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 400 GTAGAC GT^[AC][GT]AC
AlwNI 310 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 211 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 579 GAGCAC G[AT]GC[AT]^C
AvaII 529 GGTCC G^G[AT]CC
BanII 248 GAGCCC G[GA]GC[CT]^C
BcnI 532 CCCGG CC^[GC]GG
BseRI 245 GAGGAG GAGGAG(10/8)
BsiHKAI 579 GAGCAC G[AT]GC[AT]^C
BsmI 468 GAATGC GAATGC(1/-1)
BspHI 494 TCATGA T^CATGA
BstBI 218 TTCGAA TT^CGAA
BstNI 207 CCAGG CC^[AT]GG
Cac8I 202 GCAGGC GC[ACGT]^[ACGT]GC
Cfr13I 529 GGTCC G^G[ACGT]CC
DraII 528 GGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 252 CCATGG C^C[GA][CT]GG
EcoO109I 528 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 440 GAATTC G^AATTC
EcoRII 207 CCAGG ^CC[AT]GG
HaeIII 205 GGCC GG^CC
HapII 533 CCGG C^CGG
HincII 349 GTCAAC GT[CT]^[GA]AC
HindII 349 GTCAAC GT[CT]^[GA]AC
HindIII 181 AAGCTT A^AGCTT
HpaII 533 CCGG C^CGG
HphI 227 TCACC GGTGA(8/7)
MboII 81 TCTTC GAAGA(8/7)
MfeI 128 CAATTG C^AATTG
MspI 533 CCGG C^CGG
MunI 128 CAATTG C^AATTG
MvaI 207 CCAGG CC^[AT]GG
NciI 532 CCCGG CC^[GC]GG
NcoI 252 CCATGG C^CATGG
NspV 218 TTCGAA TT^CGAA
PpuMI 528 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 316 CTGCAG CTGCA^G
RcaI 494 TCATGA T^CATGA
Sau96I 529 GGTCC G^G[ACGT]CC
SfaNI 257 GCATC GCATC(5/9)
SfuI 218 TTCGAA TT^CGAA
TaqI 219 TCGA T^CGA
Tsp45I 277 GTGAC ^GT[GC]AC
TthHB8I 219 TCGA T^CGA
XmnI 211 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BmyI 248 GAGCCC G[AGT]GC[ACT]^C
579 GAGCAC
BpmI 231 CTCCAG CTGGAG(16/14)
584 CTCCAG
BsaJI 252 CCATGG C^C[ACGT][ACGT]GG
435 CCTTGG
BsmFI 194 GGGAC GGGAC(10/14)
530 GTCCC
Bsp1286I 248 GAGCCC G[AGT]GC[ACT]^C
579 GAGCAC
BssKI 207 CCAGG ^CC[ACGT]GG
532 CCCGG
EcoT14I 252 CCATGG C^C[AT][AT]GG
435 CCTTGG
MaeIII 39 GTTAC ^GT[ACGT]AC
277 GTGAC
MslI 261 CATAGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
495 CATGACGATG
MspA1I 506 CAGCTG C[AC]G^C[GT]G
542 CAGCTG
PleI 55 GACTC GAGTC(4/5)
289 GAGTC
PvuII 506 CAGCTG CAG^CTG
542 CAGCTG
ScrFI 207 CCAGG CC^[ACGT]GG
532 CCCGG
SfcI 155 CTATAG C^T[GA][CT]AG
316 CTGCAG
StyI 252 CCATGG C^C[AT][AT]GG
435 CCTTGG
TfiI 12 GAATC G^A[AT]TC
430 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AcsI AluI ApoI BbvI BsrI
DdeI DpnII Fnu4HI HinfI ItaI
MboI MnlI MseI MwoI NdeII
NlaIII NlaIV Sau3AI Tru9I TseI
Tsp509I TspRI