Restriction map for
pFN21AE1543
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvrII
BamHI BbeI BbrPI BbvCI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaHI BseAI BseRI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DpnII
DraI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MboI MfeI MflI MluI
MroI MseI MunI NarI NdeI
NdeII NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI Sau3AI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tru9I Tsp509I Tth111I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AflIII 345 ACGTGT A^C[GA][CT]GT
ApaI 1100 GGGCCC GGGCC^C
AviII 425 TGCGCA TGC^GCA
BbsI 874 GTCTTC GAAGAC(2/6)
BciVI 255 GTATCC GTATCC(6/5)
BglI 1345 GCCAGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 874 GTCTTC GAAGAC(2/6)
BsaAI 1060 TACGTG [CT]AC^GT[GA]
BsaBI 445 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1012 GAGACC GGTCTC(1/5)
BsaWI 326 ACCGGA [AT]^CCGG[AT]
BsiEI 240 CGACCG CG[GA][CT]^CG
BsmI 171 GAATGC GAATGC(1/-1)
BspHI 639 TCATGA T^CATGA
BstAPI 552 GCAGTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 975 CATCC GGATG(2/0)
DraIII 1202 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 742 CTCTTC CTCTTC(1/4)
FokI 975 CATCC GGATG(9/13)
FseI 97 GGCCGGCC GGCCGG^CC
FspI 425 TGCGCA TGC^GCA
HaeII 1121 GGCGCT [GA]GCGC^[CT]
HgaI 988 GACGC GACGC(5/10)
Ksp632I 742 CTCTTC CTCTTC(1/4)
MamI 445 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 98 GCCGGC GCC^GGC
NgoMIV 98 GCCGGC G^CCGGC
PflMI 468 CCAGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 373 GAGTC GAGTC(4/5)
PpuMI 1363 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 639 TCATGA T^CATGA
SexAI 1203 ACCTGGT A^CC[AT]GGT
TfiI 268 GATTC G^A[AT]TC
Van91I 468 CCAGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 20 CCACCGTTACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 754 GTCTAC GT^[AC][GT]AC
868 GTCTAC
AccII 238 CGCG CG^CG
990 CGCG
BcgI 765 CGAGACACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
354 GCACTACATTCG
BcnI 596 CCCGG CC^[GC]GG
1098 CCGGG
BsgI 184 GTGCAG GTGCAG(16/14)
649 GTGCAG
BspMI 1015 ACCTGC ACCTGC(4/8)
1259 ACCTGC
BsrDI 701 GCAATG GCAATG(2/0)
1308 GCAATG
BsrFI 98 GCCGGC [GA]^CCGG[CT]
455 GCCGGT
BstUI 238 CGCG CG^CG
990 CGCG
Cfr10I 98 GCCGGC [GA]^CCGG[CT]
455 GCCGGT
DrdI 366 GACACAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
505 GACCGCATAGTC
HinfI 268 GATTC G^A[ACGT]TC
373 GAGTC
MvnI 238 CGCG CG^CG
990 CGCG
NciI 596 CCCGG CC^[GC]GG
1098 CCGGG
PstI 337 CTGCAG CTGCA^G
1261 CTGCAG
SacI 64 GAGCTC GAGCT^C
1234 GAGCTC
SfaNI 333 GATGC GCATC(5/9)
1161 GCATC
SmlI 67 CTCAAG C^T[CT][GA]AG
151 CTCAAG
SspI 220 AATATT AAT^ATT
1088 AATATT
TaqI 266 TCGA T^CGA
395 TCGA
Tsp45I 485 GTCAC ^GT[GC]AC
986 GTGAC
TthHB8I 266 TCGA T^CGA
395 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BalI BanI BanII BbvI BfaI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstNI BstXI Cac8I CfoI
Cfr13I DdeI DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeI MaeII MaeIII
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NcoI
NlaIII NlaIV PvuII RsaI Sau96I
ScrFI SfcI StuI StyI TseI
TspRI