Restriction map for
pFN21AE5110
##################################
# NO CUT ENZYMES ( 185 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BamHI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BlnI
BmyI BpmI BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstF5I BstPI BstUI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FokI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI HphI
KasI KpnI Ksp632I KspI MaeI
MaeII MaeIII MamI MfeI MluI
MroI MslI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tsp45I TspRI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AfaI 30 GTAC GT^AC
AvaI 452 CCCGAG C^[CT]CG[GA]G
AvaII 68 GGACC G^G[AT]CC
BalI 272 TGGCCA TGG^CCA
BanI 393 GGCACC G^G[CT][GA]CC
BcnI 43 CCCGG CC^[GC]GG
BglII 35 AGATCT A^GATCT
BlpI 352 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 352 GCTAAGC GC^T[ACGT]AGC
BsgI 15 GTGCAG GTGCAG(16/14)
BsmFI 183 GGGAC GGGAC(10/14)
BsoBI 452 CCCGAG C^[CT]CG[GA]G
BsrDI 376 GCAATG GCAATG(2/0)
BstNI 65 CCAGG CC^[AT]GG
BstYI 35 AGATCT [GA]^GATC[CT]
Cac8I 357 GCAAGC GC[ACGT]^[ACGT]GC
CelII 352 GCTAAGC GC^T[ACGT]AGC
CfoI 57 GCGC GCG^C
DpnII 36 GATC ^GATC
EaeI 272 TGGCCA [CT]^GGCC[GA]
EcoRII 65 CCAGG ^CC[AT]GG
HaeII 56 GGCGCT [GA]GCGC^[CT]
HapII 44 CCGG C^CGG
HhaI 57 GCGC GCG^C
HinP1I 57 GCGC G^CGC
HpaII 44 CCGG C^CGG
MboI 36 GATC ^GATC
MboII 193 GAAGA GAAGA(8/7)
MflI 35 AGATCT [GA]^GATC[CT]
MluNI 272 TGGCCA TGG^CCA
MscI 272 TGGCCA TGG^CCA
MseI 95 TTAA T^TAA
MspI 44 CCGG C^CGG
MvaI 65 CCAGG CC^[AT]GG
NciI 43 CCCGG CC^[GC]GG
NdeII 36 GATC ^GATC
NlaIV 393 GGCACC GG[ACGT]^[ACGT]CC
PleI 109 GAGTC GAGTC(4/5)
RsaI 30 GTAC GT^AC
Sau3AI 36 GATC ^GATC
SfcI 162 CTACAG C^T[GA][CT]AG
TaqI 8 TCGA T^CGA
Tru9I 95 TTAA T^TAA
TthHB8I 8 TCGA T^CGA
XhoII 35 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AciI 216 CCGC CCGC(-3/-1)
26 GCGG
AcsI 197 AAATTC [GA]^AATT[CT]
229 AAATTC
ApoI 197 AAATTC [GA]^AATT[CT]
229 AAATTC
Asp700I 78 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
193 GAAGAAATTC
BsaJI 42 CCCCGG C^C[ACGT][ACGT]GG
136 CCTCGG
BssKI 43 CCCGG ^CC[ACGT]GG
65 CCAGG
BstAPI 253 GCATCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
376 GCAATGGCTGC
Cfr13I 68 GGACC G^G[ACGT]CC
239 GGGCC
FauI 215 CCCGC CCCGC(4/6)
26 GCGGG
HaeIII 240 GGCC GG^CC
273 GGCC
Sau96I 68 GGACC G^G[ACGT]CC
239 GGGCC
ScrFI 43 CCCGG CC^[ACGT]GG
65 CCAGG
SfaNI 253 GCATC GCATC(5/9)
361 GCATC
TfiI 132 GAATC G^A[AT]TC
282 GAATC
Tsp509I 198 AATT ^AATT
230 AATT
XmnI 78 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
193 GAAGAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
AluI BbvI BsiYI BslI BsrI
DdeI Fnu4HI HinfI ItaI MnlI
MwoI TseI