Restriction map for
pFN21AE1352
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AviII AvrII BamHI BbrPI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BseAI BsiEI BsiHKAI BsiWI BsmI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT14I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MaeI MamI MfeI
MroI MspA1I MunI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I StyI
SwaI Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AcsI 324 AAATTT [GA]^AATT[CT]
AfaI 438 GTAC GT^AC
AflIII 713 ACGCGT A^C[GA][CT]GT
ApoI 324 AAATTT [GA]^AATT[CT]
AspI 379 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 860 CCCGAG C^[CT]CG[GA]G
AvaII 491 GGTCC G^G[AT]CC
BalI 398 TGGCCA TGG^CCA
BanII 64 GAGCCC G[GA]GC[CT]^C
BbeI 39 GGCGCC GGCGC^C
BbvCI 901 GCTGAGG CCTCAGC(-5/-2)
BciVI 839 GTATCC GTATCC(6/5)
BmyI 64 GAGCCC G[AGT]GC[ACT]^C
BsaI 585 GGTCTC GGTCTC(1/5)
BsaWI 776 ACCGGA [AT]^CCGG[AT]
BsmBI 121 CGTCTC CGTCTC(1/5)
BsoBI 860 CCCGAG C^[CT]CG[GA]G
Bsp1286I 64 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 437 TGTACA T^GTACA
BsrGI 437 TGTACA T^GTACA
BsrI 81 ACTGG ACTGG(1/-1)
BstF5I 579 GGATG GGATG(2/0)
BstXI 68 CCATCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 189 GGATCT [GA]^GATC[CT]
CfoI 40 GCGC GCG^C
DsaI 47 CCGTGG C^C[GA][CT]GG
EarI 133 CTCTTC CTCTTC(1/4)
EcoNI 482 CCTGTGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 523 ATGCAT ATGCA^T
FokI 579 GGATG GGATG(9/13)
HaeII 39 GGCGCC [GA]GCGC^[CT]
HhaI 40 GCGC GCG^C
HinP1I 40 GCGC G^CGC
HinfI 462 GATTC G^A[ACGT]TC
KasI 39 GGCGCC G^GCGCC
Ksp632I 133 CTCTTC CTCTTC(1/4)
MaeII 473 ACGT A^CGT
MflI 189 GGATCT [GA]^GATC[CT]
MluI 713 ACGCGT A^CGCGT
MluNI 398 TGGCCA TGG^CCA
MscI 398 TGGCCA TGG^CCA
MslI 642 CACAGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 91 GCCGGC GCC^GGC
NarI 39 GGCGCC GG^CGCC
NgoMIV 91 GCCGGC G^CCGGC
NsiI 523 ATGCAT ATGCA^T
PflFI 379 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 181 CTGCAG CTGCA^G
RsaI 438 GTAC GT^AC
SexAI 572 ACCTGGT A^CC[AT]GGT
SfcI 181 CTGCAG C^T[GA][CT]AG
SfoI 39 GGCGCC GGC^GCC
SphI 525 GCATGC GCATG^C
SspBI 437 TGTACA T^GTACA
SspI 352 AATATT AAT^ATT
StuI 531 AGGCCT AGG^CCT
TfiI 462 GATTC G^A[AT]TC
Tsp45I 377 GTGAC ^GT[GC]AC
Tth111I 379 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 189 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 119 CGCG CG^CG
714 CGCG
AcyI 39 GGCGCC G[GA]^CG[CT]C
94 GGCGTC
BanI 39 GGCGCC G^G[CT][GA]CC
313 GGCACC
BbsI 685 GAAGAC GAAGAC(2/6)
880 GAAGAC
BcgI 320 GCAAAAATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
335 GCAAGATCATCG
BcnI 12 CCGGG CC^[GC]GG
171 CCGGG
BpmI 626 CTGGAG CTGGAG(16/14)
663 CTGGAG
BpuAI 685 GAAGAC GAAGAC(2/6)
880 GAAGAC
BsaHI 39 GGCGCC G[GA]^CG[CT]C
94 GGCGTC
BsgI 298 CTGCAC GTGCAG(16/14)
52 GTGCAG
BsmAI 122 GTCTC GTCTC(1/5)
586 GTCTC
BsmFI 78 GGGAC GGGAC(10/14)
858 GTCCC
BsrDI 404 CATTGC GCAATG(2/0)
620 GCAATG
BstUI 119 CGCG CG^CG
714 CGCG
EaeI 89 TGGCCG [CT]^GGCC[GA]
398 TGGCCA
FauI 117 CCCGC CCCGC(4/6)
149 GCGGG
HgaI 95 GCGTC GACGC(5/10)
120 GCGTC
Hin1I 39 GGCGCC G[GA]^CG[CT]C
94 GGCGTC
MaeIII 377 GTGAC ^GT[ACGT]AC
450 GTTAC
MseI 238 TTAA T^TAA
769 TTAA
MvnI 119 CGCG CG^CG
714 CGCG
NciI 12 CCGGG CC^[GC]GG
171 CCGGG
NspI 417 ACATGC [GA]CATG^[CT]
525 GCATGC
TaqI 465 TCGA T^CGA
560 TCGA
Tru9I 238 TTAA T^TAA
769 TTAA
TthHB8I 465 TCGA T^CGA
560 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BseRI BsiYI BslI BsrFI BssKI
BstNI Cac8I Cfr10I Cfr13I DdeI
DpnII EcoRII Fnu4HI HaeIII HapII
HpaII HphI ItaI MboI MboII
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TseI Tsp509I TspRI