Restriction map for
pFN21AE1424
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AviII
AvrII BbeI BbrPI BbvCI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsmBI BsmI
BspEI BspHI BspLU11I BspMI BsrFI
BssHII BssSI Bst1107I BstUI BstZ17I
Bsu36I CelII Cfr10I CpoI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoT22I FauI FbaI FseI FspI
HgaI Hin1I KasI KpnI KspI
MfeI MluI MroI MslI MspA1I
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I StuI SwaI Tth111I Van91I
XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AclI 61 AACGTT AA^CGTT
Aor51HI 1166 AGCGCT AGC^GCT
AseI 445 ATTAAT AT^TAAT
AsnI 445 ATTAAT AT^TAAT
Asp700I 266 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 992 TGGCCA TGG^CCA
BamHI 661 GGATCC G^GATCC
BanI 844 GGTGCC G^G[CT][GA]CC
BanII 1655 GAGCCC G[GA]GC[CT]^C
BbsI 468 GTCTTC GAAGAC(2/6)
BcgI 364 CGACTTGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1228 CCCGG CC^[GC]GG
BfaI 1354 CTAG C^TAG
BglII 1383 AGATCT A^GATCT
BmyI 1655 GAGCCC G[AGT]GC[ACT]^C
BpuAI 468 GTCTTC GAAGAC(2/6)
BsaAI 1130 TACGTG [CT]AC^GT[GA]
BsaBI 1083 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1292 GGTCTC GGTCTC(1/5)
BsiWI 1108 CGTACG C^GTACG
Bsp1286I 1655 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 747 TGTACA T^GTACA
BspDI 1119 ATCGAT AT^CGAT
BsrBI 51 CCGCTC CCGCTC(-3/-3)
BsrGI 747 TGTACA T^GTACA
BstBI 1574 TTCGAA TT^CGAA
BstEII 25 GGTGACC G^GT[ACGT]ACC
BstPI 25 GGTGACC G^GT[ACGT]ACC
BstXI 1379 CCACAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1167 GCGC GCG^C
ClaI 1119 ATCGAT AT^CGAT
DraI 933 TTTAAA TTT^AAA
DraIII 1184 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 459 GACATCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 600 CCATGG C^C[GA][CT]GG
EaeI 992 TGGCCA [CT]^GGCC[GA]
Eco47III 1166 AGCGCT AGC^GCT
EcoO65I 25 GGTGACC G^GT[ACGT]ACC
EcoRI 821 GAATTC G^AATTC
EcoRV 805 GATATC GAT^ATC
HaeII 1166 AGCGCT [GA]GCGC^[CT]
HapII 1229 CCGG C^CGG
HhaI 1167 GCGC GCG^C
HinP1I 1167 GCGC G^CGC
HpaII 1229 CCGG C^CGG
MaeI 1354 CTAG C^TAG
MamI 1083 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 992 TGGCCA TGG^CCA
MscI 992 TGGCCA TGG^CCA
MspI 1229 CCGG C^CGG
NciI 1228 CCCGG CC^[GC]GG
NcoI 600 CCATGG C^CATGG
NspI 671 GCATGC [GA]CATG^[CT]
NspV 1574 TTCGAA TT^CGAA
PaeR7I 1484 CTCGAG C^TCGAG
PpuMI 941 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 445 ATTAAT AT^TAAT
Psp1406I 61 AACGTT AA^CGTT
SfuI 1574 TTCGAA TT^CGAA
SmlI 1484 CTCGAG C^T[CT][GA]AG
SphI 671 GCATGC GCATG^C
SspBI 747 TGTACA T^GTACA
Tsp45I 26 GTGAC ^GT[GC]AC
XhoI 1484 CTCGAG C^TCGAG
XmnI 266 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
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AciI 30 CCGC CCGC(-3/-1)
51 CCGC
AlwNI 764 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
1553 CAGAAGCTG
AvaI 1295 CTCGGG C^[CT]CG[GA]G
1484 CTCGAG
BsiYI 1228 CCCGGTGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1283 CCAGCATGGGG
BslI 1228 CCCGGTGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1283 CCAGCATGGGG
BsmAI 1487 GAGAC GTCTC(1/5)
1293 GTCTC
BsmFI 941 GGGAC GGGAC(10/14)
1035 GGGAC
BsoBI 1295 CTCGGG C^[CT]CG[GA]G
1484 CTCGAG
DdeI 455 CTAAG C^T[ACGT]AG
1100 CTAAG
DraII 6 GGGGCCT [GA]G^G[ACGT]CC[CT]
941 GGGACCT
EcoO109I 6 GGGGCCT [GA]G^G[ACGT]CC[CT]
941 GGGACCT
EcoT14I 600 CCATGG C^C[AT][AT]GG
812 CCAAGG
HindIII 739 AAGCTT A^AGCTT
962 AAGCTT
HpaI 412 GTTAAC GTT^AAC
1426 GTTAAC
PleI 1375 GACTC GAGTC(4/5)
1210 GAGTC
PstI 71 CTGCAG CTGCA^G
209 CTGCAG
SspI 88 AATATT AAT^ATT
586 AATATT
StyI 600 CCATGG C^C[AT][AT]GG
812 CCAAGG
TfiI 683 GAATC G^A[AT]TC
799 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbvI BpmI BsaJI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I Cfr13I DpnII EarI
EcoRII Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII