Restriction map for
pFN21AE4463
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI AspI
AviII AvrII BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII ClaI CpoI DraI DraII
DraIII DsaI Eco47III EcoNI EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KspI MaeI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NgoMIV
NheI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SexAI SfoI SfuI
SgrAI SmlI SnaBI SpeI SspBI
SspI StuI StyI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 107 GGTACC G^GTACC
AccI 85 GTCTAC GT^[AC][GT]AC
AcyI 77 GACGCC G[GA]^CG[CT]C
AflIII 152 ACACGT A^C[GA][CT]GT
AhdI 77 GACGCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 107 GGTACC G^GTACC
AspEI 77 GACGCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 517 GAGCTC G[AT]GC[AT]^C
BalI 50 TGGCCA TGG^CCA
BamHI 1182 GGATCC G^GATCC
BbsI 790 GAAGAC GAAGAC(2/6)
BglI 463 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 790 GAAGAC GAAGAC(2/6)
BsaAI 46 TACGTG [CT]AC^GT[GA]
BsaHI 77 GACGCC G[GA]^CG[CT]C
BsiHKAI 517 GAGCTC G[AT]GC[AT]^C
BsmI 832 GCATTC GAATGC(1/-1)
BspMI 110 ACCTGC ACCTGC(4/8)
BsrBI 473 CCGCTC CCGCTC(-3/-3)
BsrFI 1037 ACCGGC [GA]^CCGG[CT]
BstAPI 823 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1182 GGATCC [GA]^GATC[CT]
Cfr10I 1037 ACCGGC [GA]^CCGG[CT]
DrdI 1324 GACAAGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 77 GACGCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1265 CTCTTC CTCTTC(1/4)
EcoRI 13 GAATTC G^AATTC
Hin1I 77 GACGCC G[GA]^CG[CT]C
KpnI 107 GGTACC GGTAC^C
Ksp632I 1265 CTCTTC CTCTTC(1/4)
MflI 1182 GGATCC [GA]^GATC[CT]
MluNI 50 TGGCCA TGG^CCA
MscI 50 TGGCCA TGG^CCA
NdeI 795 CATATG CA^TATG
NotI 114 GCGGCCGC GC^GGCCGC
PvuII 1147 CAGCTG CAG^CTG
SacI 517 GAGCTC GAGCT^C
SapI 1264 GCTCTTC GCTCTTC(1/4)
SbfI 663 CCTGCAGG CCTGCA^GG
SfiI 462 GGCCCTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 103 CCCGGG CCC^GGG
SphI 328 GCATGC GCATG^C
Sse8387I 663 CCTGCAGG CCTGCA^GG
XhoII 1182 GGATCC [GA]^GATC[CT]
XmaCI 103 CCCGGG C^CCGGG
XmaI 103 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 13 GAATTC [GA]^AATT[CT]
921 GAATTT
AfaI 108 GTAC GT^AC
255 GTAC
AocI 139 CCTCAGG CC^T[ACGT]AGG
1317 CCTCAGG
ApoI 13 GAATTC [GA]^AATT[CT]
921 GAATTT
Asp700I 1207 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1261 GAAGCTCTTC
AvaI 103 CCCGGG C^[CT]CG[GA]G
364 CTCGGG
BanI 107 GGTACC G^G[CT][GA]CC
854 GGCACC
BanII 100 GAGCCC G[GA]GC[CT]^C
517 GAGCTC
BmyI 100 GAGCCC G[AGT]GC[ACT]^C
517 GAGCTC
BseRI 576 CTCCTC GAGGAG(10/8)
422 GAGGAG
BsgI 802 CTGCAC GTGCAG(16/14)
1378 GTGCAG
BsiEI 115 CGGCCG CG[GA][CT]^CG
1412 CGGCCG
BsmAI 343 GAGAC GTCTC(1/5)
683 GAGAC
BsoBI 103 CCCGGG C^[CT]CG[GA]G
364 CTCGGG
Bsp1286I 100 GAGCCC G[AGT]GC[ACT]^C
517 GAGCTC
BstXI 53 CCAACTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
452 CCACCCTGGTGG
Bsu36I 139 CCTCAGG CC^T[ACGT]AGG
1317 CCTCAGG
CfoI 1176 GCGC GCG^C
1338 GCGC
EagI 115 CGGCCG C^GGCCG
1412 CGGCCG
EclXI 115 CGGCCG C^GGCCG
1412 CGGCCG
Eco52I 115 CGGCCG C^GGCCG
1412 CGGCCG
Eco81I 139 CCTCAGG CC^T[ACGT]AGG
1317 CCTCAGG
HhaI 1176 GCGC GCG^C
1338 GCGC
HinP1I 1176 GCGC G^CGC
1338 GCGC
PleI 1109 GAGTC GAGTC(4/5)
1165 GAGTC
RsaI 108 GTAC GT^AC
255 GTAC
XmnI 1207 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1261 GAAGCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BcnI BpmI BsaJI BsiYI BslI
BsmFI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EaeI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PstI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI