Restriction map for
pFN21AE3669
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AgeI Alw44I AlwNI AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspHI AvrII
BalI BbvCI BciVI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BseRI BsgI BsiHKAI BsiWI BsmI
BspHI BspLU11I BspMI BsrDI BssHII
Bst1107I BstBI BstZ17I Bsu36I CelII
CpoI DraI DraIII Eco81I EcoRI
EcoRV EcoT14I EcoT22I FseI HincII
HindII HindIII HpaI KpnI KspI
MaeI MamI MfeI MluI MluNI
MscI MseI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspI StuI StyI SwaI TfiI
Tru9I Tsp509I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccIII 52 TCCGGA T^CCGGA
AhdI 982 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 494 AGCGCT AGC^GCT
AspEI 982 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 916 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1047 CCCGGG C^[CT]CG[GA]G
AvaII 110 GGACC G^G[AT]CC
AviII 331 TGCGCA TGC^GCA
BamHI 154 GGATCC G^GATCC
BanII 607 GAGCCC G[GA]GC[CT]^C
BbeI 1339 GGCGCC GGCGC^C
BbrPI 43 CACGTG CAC^GTG
BbsI 12 GAAGAC GAAGAC(2/6)
BcgI 209 GCATCACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 305 TGATCA T^GATCA
BglI 409 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 12 GAAGAC GAAGAC(2/6)
BsaAI 43 CACGTG [CT]AC^GT[GA]
BsaWI 52 TCCGGA [AT]^CCGG[AT]
BseAI 52 TCCGGA T^CCGGA
BsmBI 1190 GAGACG CGTCTC(1/5)
BsoBI 1047 CCCGGG C^[CT]CG[GA]G
Bsp1407I 758 TGTACA T^GTACA
BspDI 268 ATCGAT AT^CGAT
BspEI 52 TCCGGA T^CCGGA
BsrBI 1332 GAGCGG CCGCTC(-3/-3)
BsrGI 758 TGTACA T^GTACA
BssSI 886 CACGAG CACGAG(-5/-1)
BstAPI 405 GCATGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1226 CCATGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 154 GGATCC [GA]^GATC[CT]
ClaI 268 ATCGAT AT^CGAT
DraII 689 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 502 GACGAGATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 982 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1329 GAAGAG CTCTTC(1/4)
Eco47III 494 AGCGCT AGC^GCT
EcoNI 681 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 689 AGGCCCT [GA]G^G[ACGT]CC[CT]
FbaI 305 TGATCA T^GATCA
FspI 331 TGCGCA TGC^GCA
KasI 1339 GGCGCC G^GCGCC
Ksp632I 1329 GAAGAG CTCTTC(1/4)
MflI 154 GGATCC [GA]^GATC[CT]
MroI 52 TCCGGA T^CCGGA
MspA1I 745 CCGCTG C[AC]G^C[GT]G
NarI 1339 GGCGCC GG^CGCC
PflFI 916 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 43 CACGTG CAC^GTG
PmlI 43 CACGTG CAC^GTG
PstI 748 CTGCAG CTGCA^G
SapI 1329 GAAGAGC GCTCTTC(1/4)
ScaI 251 AGTACT AGT^ACT
SfoI 1339 GGCGCC GGC^GCC
SmaI 1047 CCCGGG CCC^GGG
SphI 405 GCATGC GCATG^C
SspBI 758 TGTACA T^GTACA
Tth111I 916 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1199 CCATCGTGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 154 GGATCC [GA]^GATC[CT]
XmaCI 1047 CCCGGG C^CCGGG
XmaI 1047 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AcyI 1306 GGCGTC G[GA]^CG[CT]C
1339 GGCGCC
AlwI 155 GATCC GGATC(4/5)
154 GGATC
BsaHI 1306 GGCGTC G[GA]^CG[CT]C
1339 GGCGCC
BsaI 241 GAGACC GGTCTC(1/5)
802 GAGACC
BsiEI 817 CGGCCG CG[GA][CT]^CG
1263 CGGCCG
BsrI 844 CCAGT ACTGG(1/-1)
1248 CCAGT
BstEII 831 GGTGACC G^GT[ACGT]ACC
1350 GGTCACC
BstPI 831 GGTGACC G^GT[ACGT]ACC
1350 GGTCACC
EagI 817 CGGCCG C^GGCCG
1263 CGGCCG
EclXI 817 CGGCCG C^GGCCG
1263 CGGCCG
Eco52I 817 CGGCCG C^GGCCG
1263 CGGCCG
EcoO65I 831 GGTGACC G^GT[ACGT]ACC
1350 GGTCACC
Hin1I 1306 GGCGTC G[GA]^CG[CT]C
1339 GGCGCC
HinfI 49 GACTC G^A[ACGT]TC
466 GACTC
PleI 49 GACTC GAGTC(4/5)
466 GACTC
SfcI 483 CTACAG C^T[GA][CT]AG
748 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
BanI BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EaeI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MslI MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI Tsp45I TspRI TthHB8I