Restriction map for
pFN21AE5712
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# NO CUT ENZYMES ( 97 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BamHI BbeI BbrPI BcgI
BciVI BfrI BglII BlnI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrGI BssHII
BssSI BstBI ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoRI EcoRV EcoT22I FseI HgaI
HincII HindII HindIII HpaI KasI
KpnI KspI MluI MroI MseI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI ScaI SfoI SfuI
SgrAI SnaBI SphI SspBI SspI
StuI SwaI Tru9I Tth111I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AatII 547 GACGTC GACGT^C
AccII 72 CGCG CG^CG
AcyI 547 GACGTC G[GA]^CG[CT]C
Alw44I 136 GTGCAC G^TGCAC
AocI 1981 CCTCAGG CC^T[ACGT]AGG
Aor51HI 408 AGCGCT AGC^GCT
ApaLI 136 GTGCAC G^TGCAC
Asp700I 351 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 317 TGCGCA TGC^GCA
BalI 1629 TGGCCA TGG^CCA
BanI 298 GGCACC G^G[CT][GA]CC
BbvCI 1932 CCTCAGC CCTCAGC(-5/-2)
BclI 1838 TGATCA T^GATCA
BfaI 1600 CTAG C^TAG
BsaAI 850 TACGTG [CT]AC^GT[GA]
BsaBI 1839 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 547 GACGTC G[GA]^CG[CT]C
BsgI 1429 GTGCAG GTGCAG(16/14)
BsmI 2160 GAATGC GAATGC(1/-1)
BspHI 2156 TCATGA T^CATGA
BsrDI 1658 GCAATG GCAATG(2/0)
Bst1107I 1008 GTATAC GTA^TAC
BstUI 72 CGCG CG^CG
BstYI 1073 GGATCT [GA]^GATC[CT]
BstZ17I 1008 GTATAC GTA^TAC
Bsu36I 1981 CCTCAGG CC^T[ACGT]AGG
DraIII 141 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 408 AGCGCT AGC^GCT
Eco81I 1981 CCTCAGG CC^T[ACGT]AGG
FauI 70 CCCGC CCCGC(4/6)
FbaI 1838 TGATCA T^GATCA
FspI 317 TGCGCA TGC^GCA
Hin1I 547 GACGTC G[GA]^CG[CT]C
MaeI 1600 CTAG C^TAG
MamI 1839 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1241 CAATTG C^AATTG
MflI 1073 GGATCT [GA]^GATC[CT]
MluNI 1629 TGGCCA TGG^CCA
MscI 1629 TGGCCA TGG^CCA
MunI 1241 CAATTG C^AATTG
MvnI 72 CGCG CG^CG
NaeI 1151 GCCGGC GCC^GGC
NgoMIV 1151 GCCGGC G^CCGGC
PshAI 928 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2156 TCATGA T^CATGA
SacI 877 GAGCTC GAGCT^C
SbfI 241 CCTGCAGG CCTGCA^GG
SexAI 200 ACCTGGT A^CC[AT]GGT
SfiI 1815 GGCCTGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 263 CCCGGG CCC^GGG
SpeI 1599 ACTAGT A^CTAGT
Sse8387I 241 CCTGCAGG CCTGCA^GG
TfiI 185 GATTC G^A[AT]TC
Tsp509I 1242 AATT ^AATT
XhoII 1073 GGATCT [GA]^GATC[CT]
XmaCI 263 CCCGGG C^CCGGG
XmaI 263 CCCGGG C^CCGGG
XmnI 351 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 943 GTCTAC GT^[AC][GT]AC
1008 GTATAC
AvaI 28 CCCGAG C^[CT]CG[GA]G
263 CCCGGG
BbsI 628 GAAGAC GAAGAC(2/6)
1989 GAAGAC
BglI 1816 GCCTGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2085 GCCTGTTCGGC
BpuAI 628 GAAGAC GAAGAC(2/6)
1989 GAAGAC
BsaI 259 GAGACC GGTCTC(1/5)
1804 GAGACC
BsoBI 28 CCCGAG C^[CT]CG[GA]G
263 CCCGGG
BsrFI 1151 GCCGGC [GA]^CCGG[CT]
2024 ACCGGC
BstAPI 235 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1913 GCAGTGACTGC
BstEII 102 GGTCACC G^GT[ACGT]ACC
1017 GGTTACC
BstPI 102 GGTCACC G^GT[ACGT]ACC
1017 GGTTACC
BstXI 536 CCAAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1782 CCACTTGATTGG
Cfr10I 1151 GCCGGC [GA]^CCGG[CT]
2024 ACCGGC
EaeI 120 CGGCCA [CT]^GGCC[GA]
1629 TGGCCA
EarI 906 CTCTTC CTCTTC(1/4)
346 GAAGAG
EcoNI 1315 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1353 CCTGGAGGAGG
EcoO65I 102 GGTCACC G^GT[ACGT]ACC
1017 GGTTACC
Ksp632I 906 CTCTTC CTCTTC(1/4)
346 GAAGAG
NcoI 1502 CCATGG C^CATGG
1761 CCATGG
PflMI 195 CCAATACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1119 CCAGAGAGTGG
TaqI 183 TCGA T^CGA
686 TCGA
TthHB8I 183 TCGA T^CGA
686 TCGA
Van91I 195 CCAATACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1119 CCAGAGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaII BanII BbvI
BcnI BlpI BmyI BpmI Bpu1102I
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TseI Tsp45I TspRI