##################################
# NO CUT ENZYMES (  189 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BclI        BcnI        
BfrI        BglI        BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmBI       
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT22I     
FauI        FbaI        FokI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HpaII       KasI        KpnI        
KspI        MaeII       MaeIII      MamI        MluI        
MluNI       MroI        MscI        MspI        MvaI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        ScrFI       SexAI       
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        SwaI        TaqI        
Tsp45I      TspRI       Tth111I     TthHB8I     Van91I      
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AcsI          231  GAATTT               [GA]^AATT[CT]
AlwNI          31  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          231  GAATTT               [GA]^AATT[CT]
AseI          529  ATTAAT               AT^TAAT
AsnI          529  ATTAAT               AT^TAAT
Asp700I       673  GAATGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         879  GGTCC                G^G[AT]CC
BbsI          677  GTCTTC               GAAGAC(2/6)
BbvI          299  GCTGC                GCAGC(8/12)
BciVI         733  GTATCC               GTATCC(6/5)
BpuAI         677  GTCTTC               GAAGAC(2/6)
BsaJI         279  CCTTGG               C^C[ACGT][ACGT]GG
BsmI           51  GAATGC               GAATGC(1/-1)
BsrI          237  ACTGG                ACTGG(1/-1)
Cfr13I        879  GGTCC                G^G[ACGT]CC
EarI          328  GAAGAG               CTCTTC(1/4)
EcoT14I       279  CCTTGG               C^C[AT][AT]GG
Fnu4HI        299  GCTGC                GC^[ACGT]GC
HaeIII        130  GGCC                 GG^CC
HincII        657  GTTAAC               GT[CT]^[GA]AC
HindII        657  GTTAAC               GT[CT]^[GA]AC
HpaI          657  GTTAAC               GTT^AAC
HphI          855  TCACC                GGTGA(8/7)
ItaI          299  GCTGC                GC^[ACGT]GC
Ksp632I       328  GAAGAG               CTCTTC(1/4)
MfeI          826  CAATTG               C^AATTG
MspA1I         46  CAGCTG               C[AC]G^C[GT]G
MunI          826  CAATTG               C^AATTG
NlaIII        563  CATG                 CATG^
NlaIV         879  GGTCCC               GG[ACGT]^[ACGT]CC
NspI          562  GCATGT               [GA]CATG^[CT]
PshBI         529  ATTAAT               AT^TAAT
PvuII          46  CAGCTG               CAG^CTG
Sau96I        879  GGTCC                G^G[ACGT]CC
SfcI          843  CTACAG               C^T[GA][CT]AG
SspI          752  AATATT               AAT^ATT
StyI          279  CCTTGG               C^C[AT][AT]GG
TfiI          245  GATTC                G^A[AT]TC
TseI          299  GCTGC                G^C[AT]GC
XbaI           73  TCTAGA               T^CTAGA
XmnI          673  GAATGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    5 enzymes) #
###################################

BglII         171  AGATCT               A^GATCT
              708  AGATCT              
BsgI          116  CTGCAC               GTGCAG(16/14)
              818  CTGCAC              
BsmAI         102  GAGAC                GTCTC(1/5)
              211  GAGAC               
BsrDI         568  CATTGC               GCAATG(2/0)
              621  CATTGC              
MslI          121  CACGATAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              641  CACAGAAGTG          


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AfaI        AluI        AlwI        BfaI        BsmFI       
BstYI       DdeI        DpnII       HinfI       MaeI        
MboI        MboII       MflI        MnlI        MseI        
MwoI        NdeII       PleI        RsaI        Sau3AI      
Tru9I       Tsp509I     XhoII