Restriction map for
pFN21AE4328
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcyI AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BglI BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaHI
BsaI BseAI BseRI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspMI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstAPI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FauI
FseI FspI HaeII HaeIII HhaI
Hin1I HinP1I HincII HindII HpaI
KasI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AflII 774 CTTAAG C^TTAAG
AflIII 241 ACATGT A^C[GA][CT]GT
AlwNI 641 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
BclI 800 TGATCA T^GATCA
BcnI 56 CCCGG CC^[GC]GG
BfaI 293 CTAG C^TAG
BfrI 774 CTTAAG C^TTAAG
BglII 109 AGATCT A^GATCT
BsaWI 380 ACCGGA [AT]^CCGG[AT]
BsgI 8 CTGCAC GTGCAG(16/14)
BsmBI 222 GAGACG CGTCTC(1/5)
BspHI 350 TCATGA T^CATGA
BspLU11I 241 ACATGT A^CATGT
BsrDI 141 GCAATG GCAATG(2/0)
BssSI 611 CTCGTG CACGAG(-5/-1)
BstBI 19 TTCGAA TT^CGAA
BstXI 78 CCAAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
FbaI 800 TGATCA T^GATCA
HphI 363 TCACC GGTGA(8/7)
MaeI 293 CTAG C^TAG
MslI 153 CATAAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 56 CCCGG CC^[GC]GG
NspV 19 TTCGAA TT^CGAA
RcaI 350 TCATGA T^CATGA
SexAI 639 ACCAGGT A^CC[AT]GGT
SfaNI 655 GATGC GCATC(5/9)
SfuI 19 TTCGAA TT^CGAA
SmlI 774 CTTAAG C^T[CT][GA]AG
XcmI 106 CCAAGATCTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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Acc65I 247 GGTACC G^GTACC
371 GGTACC
AlwI 82 GGATC GGATC(4/5)
90 GGATC
Asp718I 247 GGTACC G^GTACC
371 GGTACC
AvaII 26 GGACC G^G[AT]CC
567 GGACC
BanI 247 GGTACC G^G[CT][GA]CC
371 GGTACC
BbsI 237 GAAGAC GAAGAC(2/6)
820 GTCTTC
BpmI 734 CTCCAG CTGGAG(16/14)
40 CTGGAG
BpuAI 237 GAAGAC GAAGAC(2/6)
820 GTCTTC
BsmAI 222 GAGAC GTCTC(1/5)
214 GTCTC
Cac8I 288 GCTTGC GC[ACGT]^[ACGT]GC
885 GCTTGC
Cfr13I 26 GGACC G^G[ACGT]CC
567 GGACC
EcoRI 456 GAATTC G^AATTC
623 GAATTC
HapII 57 CCGG C^CGG
381 CCGG
HgaI 70 GACGC GACGC(5/10)
918 GCGTC
HinfI 313 GACTC G^A[ACGT]TC
498 GAGTC
HpaII 57 CCGG C^CGG
381 CCGG
KpnI 247 GGTACC GGTAC^C
371 GGTACC
MaeII 225 ACGT A^CGT
450 ACGT
MspI 57 CCGG C^CGG
381 CCGG
MwoI 885 GCTTGCTTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
987 GCAACAACAGC
NspI 206 GCATGT [GA]CATG^[CT]
241 ACATGT
PleI 313 GACTC GAGTC(4/5)
498 GAGTC
Sau96I 26 GGACC G^G[ACGT]CC
567 GGACC
TaqI 20 TCGA T^CGA
685 TCGA
TthHB8I 20 TCGA T^CGA
685 TCGA
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BsaJI BsmFI BsrI BssKI BstF5I
BstNI BstYI DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HindIII ItaI
MaeIII MboI MboII MflI MnlI
MseI MvaI NdeII NlaIII NlaIV
RsaI Sau3AI ScrFI StyI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII