Restriction map for
pFN21AE0917
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BclI BfrI BglI
BlnI BpmI BpuAI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BsrFI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HaeIII HhaI
HinP1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MfeI
MluI MluNI MroI MscI MseI
MslI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tru9I TspRI Tth111I Van91I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 614 GAATTT [GA]^AATT[CT]
AcyI 314 GGCGTC G[GA]^CG[CT]C
AfaI 381 GTAC GT^AC
AhdI 454 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 614 GAATTT [GA]^AATT[CT]
AspEI 454 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 65 GTGCTC G[AT]GC[AT]^C
BciVI 817 GGATAC GTATCC(6/5)
BcnI 206 CCCGG CC^[GC]GG
BlpI 372 GCTGAGC GC^T[ACGT]AGC
BmyI 65 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 372 GCTGAGC GC^T[ACGT]AGC
BsaAI 242 CACGTA [CT]AC^GT[GA]
BsaHI 314 GGCGTC G[GA]^CG[CT]C
BsiHKAI 65 GTGCTC G[AT]GC[AT]^C
Bsp1286I 65 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 380 TGTACA T^GTACA
BspMI 248 GCAGGT ACCTGC(4/8)
BsrDI 429 CATTGC GCAATG(2/0)
BsrGI 380 TGTACA T^GTACA
BstEII 788 GGTCACC G^GT[ACGT]ACC
BstF5I 745 GGATG GGATG(2/0)
BstPI 788 GGTCACC G^GT[ACGT]ACC
BstXI 536 CCAATGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 372 GCTGAGC GC^T[ACGT]AGC
DraII 151 AGGTCCC [GA]G^G[ACGT]CC[CT]
Eam1105I 454 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 151 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 788 GGTCACC G^GT[ACGT]ACC
FokI 745 GGATG GGATG(9/13)
HapII 207 CCGG C^CGG
HgaI 315 GCGTC GACGC(5/10)
Hin1I 314 GGCGTC G[GA]^CG[CT]C
HpaII 207 CCGG C^CGG
MspA1I 9 CAGCGG C[AC]G^C[GT]G
MspI 207 CCGG C^CGG
NciI 206 CCCGG CC^[GC]GG
PpuMI 151 AGGTCCC [GA]G^G[AT]CC[CT]
RsaI 381 GTAC GT^AC
SfaNI 511 GATGC GCATC(5/9)
SspBI 380 TGTACA T^GTACA
Tsp509I 615 AATT ^AATT
XbaI 172 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 272 GTAGAC GT^[AC][GT]AC
303 GTCTAC
AvaII 152 GGTCC G^G[AT]CC
424 GGTCC
BbvI 641 GCAGC GCAGC(8/12)
754 GCTGC
BglII 396 AGATCT A^GATCT
835 AGATCT
BsaJI 116 CCAAGG C^C[ACGT][ACGT]GG
515 CCAAGG
BsrBI 225 CCGCTC CCGCTC(-3/-3)
31 GAGCGG
Cfr13I 152 GGTCC G^G[ACGT]CC
424 GGTCC
DdeI 373 CTGAG C^T[ACGT]AG
400 CTAAG
EcoT14I 116 CCAAGG C^C[AT][AT]GG
515 CCAAGG
FauI 224 CCCGC CCCGC(4/6)
33 GCGGG
HphI 790 TCACC GGTGA(8/7)
850 TCACC
MaeIII 210 GTCAC ^GT[ACGT]AC
789 GTCAC
MboII 121 GAAGA GAAGA(8/7)
182 GAAGA
NlaIV 152 GGTCCC GG[ACGT]^[ACGT]CC
424 GGTCCC
Sau96I 152 GGTCC G^G[ACGT]CC
424 GGTCC
SfcI 705 CTATAG C^T[GA][CT]AG
804 CTATAG
StyI 116 CCAAGG C^C[AT][AT]GG
515 CCAAGG
TaqI 261 TCGA T^CGA
840 TCGA
TseI 641 GCAGC G^C[AT]GC
754 GCTGC
Tsp45I 210 GTCAC ^GT[GC]AC
789 GTCAC
TthHB8I 261 TCGA T^CGA
840 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI AlwI BfaI BsaBI
BsaI BseRI BsiYI BslI BsmAI
BsmFI BssKI BstNI BstYI Cac8I
DpnII EcoRII Fnu4HI HinfI ItaI
MaeI MaeII MamI MboI MflI
MnlI MvaI MwoI NdeII NlaIII
Sau3AI ScrFI TfiI XhoII