Restriction map for
pFN21AE4562
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AviII AvrII BamHI
BbsI BciVI BclI BfrI BglII
BlnI BpuAI BsiWI BsmI Bsp1407I
BspDI BspHI BsrDI BsrGI BssHII
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI MfeI MluI MseI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SalI SapI SbfI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tsp509I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 281 TCCGGA T^CCGGA
ApaI 788 GGGCCC GGGCC^C
AspI 829 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1971 GCTGAGG CCTCAGC(-5/-2)
BlpI 510 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 510 GCTGAGC GC^T[ACGT]AGC
BsaBI 1326 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 281 TCCGGA [AT]^CCGG[AT]
BseAI 281 TCCGGA T^CCGGA
BsiEI 987 CGGCCG CG[GA][CT]^CG
BsmBI 1036 GAGACG CGTCTC(1/5)
BspEI 281 TCCGGA T^CCGGA
BspLU11I 1862 ACATGT A^CATGT
BsrFI 737 GCCGGT [GA]^CCGG[CT]
BssSI 1147 CACGAG CACGAG(-5/-1)
BstXI 1416 CCATGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1745 AGATCC [GA]^GATC[CT]
CelII 510 GCTGAGC GC^T[ACGT]AGC
Cfr10I 737 GCCGGT [GA]^CCGG[CT]
EagI 987 CGGCCG C^GGCCG
EarI 1645 CTCTTC CTCTTC(1/4)
EclXI 987 CGGCCG C^GGCCG
Eco52I 987 CGGCCG C^GGCCG
Ksp632I 1645 CTCTTC CTCTTC(1/4)
KspI 1249 CCGCGG CCGC^GG
MamI 1326 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1745 AGATCC [GA]^GATC[CT]
MroI 281 TCCGGA T^CCGGA
PflFI 829 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 1249 CCGCGG CCGC^GG
SfiI 1338 GGCCATGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1595 CCCGGG CCC^GGG
TfiI 936 GATTC G^A[AT]TC
Tth111I 829 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1745 AGATCC [GA]^GATC[CT]
XmaCI 1595 CCCGGG C^CCGGG
XmaI 1595 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 419 GGTACC G^GTACC
1832 GGTACC
AlwI 1746 GATCC GGATC(4/5)
362 GGATC
Asp718I 419 GGTACC G^GTACC
1832 GGTACC
BbeI 1156 GGCGCC GGCGC^C
1506 GGCGCC
BcgI 557 CGACCTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1719 CGAGGAGTATGC
BfaI 586 CTAG C^TAG
883 CTAG
BglI 1330 GCCATCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1339 GCCATGGTGGC
BsrBI 427 GAGCGG CCGCTC(-3/-3)
1489 GAGCGG
BstAPI 13 GCAGCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1098 GCACGGGCTGC
BstEII 216 GGTCACC G^GT[ACGT]ACC
648 GGTCACC
BstPI 216 GGTCACC G^GT[ACGT]ACC
648 GGTCACC
EcoO65I 216 GGTCACC G^GT[ACGT]ACC
648 GGTCACC
KasI 1156 GGCGCC G^GCGCC
1506 GGCGCC
KpnI 419 GGTACC GGTAC^C
1832 GGTACC
MaeI 586 CTAG C^TAG
883 CTAG
MslI 44 CATCTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1857 CATGGACATG
NarI 1156 GGCGCC GG^CGCC
1506 GGCGCC
NcoI 1340 CCATGG C^CATGG
1856 CCATGG
PflMI 864 CCAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1160 CCAGTGCCTGG
ScaI 1073 AGTACT AGT^ACT
1823 AGTACT
SexAI 146 ACCAGGT A^CC[AT]GGT
1025 ACCAGGT
SfoI 1156 GGCGCC GGC^GCC
1506 GGCGCC
StuI 725 AGGCCT AGG^CCT
1641 AGGCCT
TspRI 398 CACTG CA[GC]TG[ACGT][ACGT]^
1161 CAGTG
Van91I 864 CCAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1160 CCAGTGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbrPI BbvI
BcnI BmyI BpmI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PmaCI PmlI PpuMI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TseI Tsp45I TthHB8I XcmI