Restriction map for
pFN21AB6362
##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AocI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BsgI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BssSI Bst1107I
BstBI BstEII BstF5I BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI EarI Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FokI FseI Hin1I HindIII HpaI
KasI KpnI Ksp632I MfeI MflI
MluI MluNI MroI MscI MseI
MslI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SmaI SmlI SnaBI
SpeI Sse8387I SspI StuI SwaI
TaqI Tru9I Tsp509I Tth111I TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AfaI 703 GTAC GT^AC
AhdI 565 GACTACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 484 GATCC GGATC(4/5)
AlwNI 313 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 565 GACTACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 90 CTCGGG C^[CT]CG[GA]G
AviII 217 TGCGCA TGC^GCA
BcgI 129 CGACGCGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 513 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 600 CTCCTC GAGGAG(10/8)
BsmBI 459 CGTCTC CGTCTC(1/5)
BsoBI 90 CTCGGG C^[CT]CG[GA]G
Bsp1407I 702 TGTACA T^GTACA
BspMI 377 ACCTGC ACCTGC(4/8)
BsrGI 702 TGTACA T^GTACA
BstAPI 312 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DpnII 484 GATC ^GATC
DraII 102 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 472 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 327 CGGCCG [CT]^GGCC[GA]
EagI 327 CGGCCG C^GGCCG
Eam1105I 565 GACTACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 327 CGGCCG C^GGCCG
Eco52I 327 CGGCCG C^GGCCG
EcoO109I 102 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 830 CCAAGG C^C[AT][AT]GG
FspI 217 TGCGCA TGC^GCA
HphI 666 TCACC GGTGA(8/7)
MaeIII 719 GTGAC ^GT[ACGT]AC
MamI 513 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 484 GATC ^GATC
NaeI 436 GCCGGC GCC^GGC
NdeII 484 GATC ^GATC
NgoMIV 436 GCCGGC G^CCGGC
PflMI 17 CCACGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 102 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 310 CTGCAG CTGCA^G
RsaI 703 GTAC GT^AC
SacI 87 GAGCTC GAGCT^C
Sau3AI 484 GATC ^GATC
SfaNI 245 GCATC GCATC(5/9)
SgrAI 65 CGCCGGTG C[GA]^CCGG[CT]G
SspBI 702 TGTACA T^GTACA
StyI 830 CCAAGG C^C[AT][AT]GG
TfiI 663 GAATC G^A[AT]TC
Tsp45I 719 GTGAC ^GT[GC]AC
Van91I 17 CCACGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Alw44I 137 GTGCAC G^TGCAC
847 GTGCAC
Aor51HI 257 AGCGCT AGC^GCT
551 AGCGCT
ApaLI 137 GTGCAC G^TGCAC
847 GTGCAC
AvaII 103 GGTCC G^G[AT]CC
631 GGTCC
BbsI 424 GAAGAC GAAGAC(2/6)
652 GAAGAC
BcnI 193 CCGGG CC^[GC]GG
474 CCGGG
BfaI 350 CTAG C^TAG
410 CTAG
BpmI 398 CTCCAG CTGGAG(16/14)
462 CTCCAG
BpuAI 424 GAAGAC GAAGAC(2/6)
652 GAAGAC
BsiEI 304 CGACCG CG[GA][CT]^CG
327 CGGCCG
BsrFI 66 GCCGGT [GA]^CCGG[CT]
436 GCCGGC
BsrI 141 ACTGG ACTGG(1/-1)
147 CCAGT
BssHII 152 GCGCGC G^CGCGC
369 GCGCGC
Cfr10I 66 GCCGGT [GA]^CCGG[CT]
436 GCCGGC
Eco47III 257 AGCGCT AGC^GCT
551 AGCGCT
EcoNI 757 CCTTCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
811 CCTCTCCCAGG
HgaI 130 GACGC GACGC(5/10)
341 GCGTC
HincII 343 GTCAAC GT[CT]^[GA]AC
562 GTTGAC
HindII 343 GTCAAC GT[CT]^[GA]AC
562 GTTGAC
KspI 57 CCGCGG CCGC^GG
330 CCGCGG
MaeI 350 CTAG C^TAG
410 CTAG
MaeII 214 ACGT A^CGT
845 ACGT
MboII 424 GAAGA GAAGA(8/7)
652 GAAGA
NciI 193 CCGGG CC^[GC]GG
474 CCGGG
NlaIII 535 CATG CATG^
861 CATG
NspI 534 GCATGC [GA]CATG^[CT]
860 GCATGC
SacII 57 CCGCGG CCGC^GG
330 CCGCGG
SfcI 310 CTGCAG C^T[GA][CT]AG
855 CTACAG
SphI 534 GCATGC GCATG^C
860 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AluI AspHI BanII
BbvI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DsaI EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MnlI MspA1I
MspI MvaI MvnI MwoI NlaIV
PleI Sau96I ScrFI TseI TspRI