Restriction map for
pFN21AE3581
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# NO CUT ENZYMES ( 74 enzymes) #
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Acc65I AclI AcsI AflII AgeI
AocI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BamHI BbrPI BciVI BfrI BglII
BlnI BsiWI BspDI BsrDI BssSI
Bst1107I BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eco81I EcoRI
FseI FspI KpnI MfeI MluI
MunI NaeI NgoMIV NheI NotI
NruI PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RsrII SapI ScaI SexAI
SgrAI SmaI SmlI SnaBI SpeI
SspI StuI SwaI Tsp509I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccIII 3201 TCCGGA T^CCGGA
AhdI 217 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 691 GTGCAC G^TGCAC
ApaLI 691 GTGCAC G^TGCAC
AspEI 217 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 327 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3097 CCCGAG C^[CT]CG[GA]G
BbeI 21 GGCGCC GGCGC^C
BbvCI 2644 GCTGAGG CCTCAGC(-5/-2)
BcgI 1940 GCAGCGAAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 881 TGATCA T^GATCA
BglI 2281 GCCGCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2053 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2053 GCTGAGC GC^T[ACGT]AGC
BsaAI 2967 CACGTA [CT]AC^GT[GA]
BsaBI 2796 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3201 TCCGGA [AT]^CCGG[AT]
BseAI 3201 TCCGGA T^CCGGA
BsmI 2523 GAATGC GAATGC(1/-1)
BsoBI 3097 CCCGAG C^[CT]CG[GA]G
Bsp1407I 362 TGTACA T^GTACA
BspEI 3201 TCCGGA T^CCGGA
BspHI 2183 TCATGA T^CATGA
BspLU11I 134 ACATGT A^CATGT
BsrBI 2791 GAGCGG CCGCTC(-3/-3)
BsrGI 362 TGTACA T^GTACA
BssHII 30 GCGCGC G^CGCGC
BstAPI 3128 GCAAACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2870 TTCGAA TT^CGAA
BstEII 1239 GGTCACC G^GT[ACGT]ACC
BstPI 1239 GGTCACC G^GT[ACGT]ACC
BstYI 1151 AGATCC [GA]^GATC[CT]
CelII 2053 GCTGAGC GC^T[ACGT]AGC
DraII 160 AGGGCCT [GA]G^G[ACGT]CC[CT]
EagI 188 CGGCCG C^GGCCG
Eam1105I 217 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 188 CGGCCG C^GGCCG
Eco52I 188 CGGCCG C^GGCCG
EcoNI 2196 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 160 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1239 GGTCACC G^GT[ACGT]ACC
EcoRV 1561 GATATC GAT^ATC
FbaI 881 TGATCA T^GATCA
HpaI 2155 GTTAAC GTT^AAC
KasI 21 GGCGCC G^GCGCC
KspI 2020 CCGCGG CCGC^GG
MamI 2796 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1151 AGATCC [GA]^GATC[CT]
MroI 3201 TCCGGA T^CCGGA
NarI 21 GGCGCC GG^CGCC
NdeI 877 CATATG CA^TATG
NspV 2870 TTCGAA TT^CGAA
PflFI 327 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2183 TCATGA T^CATGA
SacII 2020 CCGCGG CCGC^GG
SfiI 2280 GGCCGCTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 21 GGCGCC GGC^GCC
SfuI 2870 TTCGAA TT^CGAA
SspBI 362 TGTACA T^GTACA
TfiI 1003 GATTC G^A[AT]TC
Tth111I 327 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1151 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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AatII 1794 GACGTC GACGT^C
3087 GACGTC
AccI 1420 GTCGAC GT^[AC][GT]AC
2652 GTCGAC
AflIII 134 ACATGT A^C[GA][CT]GT
2573 ACACGT
AlwI 1152 GATCC GGATC(4/5)
2712 GATCC
Aor51HI 658 AGCGCT AGC^GCT
755 AGCGCT
BanI 21 GGCGCC G^G[CT][GA]CC
500 GGCACC
BsgI 412 CTGCAC GTGCAG(16/14)
580 GTGCAG
BsiEI 188 CGGCCG CG[GA][CT]^CG
2331 CGACCG
BsmBI 703 GAGACG CGTCTC(1/5)
1279 GAGACG
BsrFI 590 ACCGGC [GA]^CCGG[CT]
2058 GCCGGT
BstXI 1101 CCACCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1958 CCATCAAGATGG
Cfr10I 590 ACCGGC [GA]^CCGG[CT]
2058 GCCGGT
Eco47III 658 AGCGCT AGC^GCT
755 AGCGCT
EcoT22I 1121 ATGCAT ATGCA^T
2797 ATGCAT
HindIII 968 AAGCTT A^AGCTT
1900 AAGCTT
NcoI 512 CCATGG C^CATGG
1826 CCATGG
NsiI 1121 ATGCAT ATGCA^T
2797 ATGCAT
PshAI 1281 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1767 GACCCTGGTC
PvuII 474 CAGCTG CAG^CTG
583 CAGCTG
SalI 1420 GTCGAC G^TCGAC
2652 GTCGAC
SbfI 858 CCTGCAGG CCTGCA^GG
1191 CCTGCAGG
SphI 548 GCATGC GCATG^C
2243 GCATGC
Sse8387I 858 CCTGCAGG CCTGCA^GG
1191 CCTGCAGG
XcmI 242 CCAGTGACCGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1826 CCATGGAGGAGCTGG
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# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaII BalI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI Tru9I TseI Tsp45I
TspRI TthHB8I Van91I