Restriction map for
pFN21AE6116
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# NO CUT ENZYMES ( 185 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanII BbrPI
BbsI BcgI BclI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
FauI FbaI FokI FseI FspI
HgaI HincII HindII HindIII HinfI
HpaI HphI KpnI Ksp632I KspI
MaeII MaeIII MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tsp45I Tth111I TthHB8I Van91I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AciI 193 GCGG CCGC(-3/-1)
AcsI 418 GAATTT [GA]^AATT[CT]
AcyI 67 GGCGCC G[GA]^CG[CT]C
Alw44I 136 GTGCAC G^TGCAC
AlwI 11 GATCC GGATC(4/5)
ApaLI 136 GTGCAC G^TGCAC
ApoI 418 GAATTT [GA]^AATT[CT]
AspHI 136 GTGCAC G[AT]GC[AT]^C
BbeI 67 GGCGCC GGCGC^C
BbvCI 76 GCTGAGG CCTCAGC(-5/-2)
BciVI 400 GGATAC GTATCC(6/5)
BcnI 106 CCCGG CC^[GC]GG
BfaI 166 CTAG C^TAG
BsaHI 67 GGCGCC G[GA]^CG[CT]C
BseRI 79 GAGGAG GAGGAG(10/8)
BsiHKAI 136 GTGCAC G[AT]GC[AT]^C
BstYI 10 AGATCC [GA]^GATC[CT]
CfoI 68 GCGC GCG^C
DpnII 11 GATC ^GATC
EcoT22I 235 ATGCAT ATGCA^T
HaeII 67 GGCGCC [GA]GCGC^[CT]
HapII 107 CCGG C^CGG
HhaI 68 GCGC GCG^C
Hin1I 67 GGCGCC G[GA]^CG[CT]C
HinP1I 68 GCGC G^CGC
HpaII 107 CCGG C^CGG
KasI 67 GGCGCC G^GCGCC
MaeI 166 CTAG C^TAG
MboI 11 GATC ^GATC
MboII 340 GAAGA GAAGA(8/7)
MflI 10 AGATCC [GA]^GATC[CT]
MseI 353 TTAA T^TAA
MslI 238 CATGATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 107 CCGG C^CGG
NarI 67 GGCGCC GG^CGCC
NciI 106 CCCGG CC^[GC]GG
NdeII 11 GATC ^GATC
NsiI 235 ATGCAT ATGCA^T
Sau3AI 11 GATC ^GATC
SfaNI 162 GCATC GCATC(5/9)
SfoI 67 GGCGCC GGC^GCC
Tru9I 353 TTAA T^TAA
XbaI 165 TCTAGA T^CTAGA
XhoII 10 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AluI 94 AGCT AG^CT
218 AGCT
BanI 67 GGCGCC G^G[CT][GA]CC
324 GGCACC
BmyI 59 GTGCCC G[AGT]GC[ACT]^C
136 GTGCAC
BsaJI 63 CCCAGG C^C[ACGT][ACGT]GG
71 CCAGGG
BsiYI 42 CCTGCTCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
300 CCATAAGGAGG
BslI 42 CCTGCTCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
300 CCATAAGGAGG
Bsp1286I 59 GTGCCC G[AGT]GC[ACT]^C
136 GTGCAC
Cfr13I 50 GGGCC G^G[ACGT]CC
117 GGGCC
PstI 86 CTGCAG CTGCA^G
433 CTGCAG
Sau96I 50 GGGCC G^G[ACGT]CC
117 GGGCC
SfcI 86 CTGCAG C^T[GA][CT]AG
433 CTGCAG
TspRI 134 CAGTG CA[GC]TG[ACGT][ACGT]^
431 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
BbvI BssKI BstNI Cac8I DdeI
EcoRII Fnu4HI HaeIII ItaI MnlI
MvaI MwoI NlaIII NlaIV ScrFI
TseI Tsp509I