Restriction map for
pFN21AE1478
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BcgI BciVI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BsgI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HinfI HpaI
KpnI KspI MamI MfeI MluI
MroI MseI MunI NcoI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
TspRI Tth111I Van91I XhoI XmnI
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# ONE CUT ENZYMES ( 24 enzymes) #
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AcsI 780 GAATTT [GA]^AATT[CT]
AflIII 1138 ACGTGT A^C[GA][CT]GT
AlwNI 910 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 12 GGGCCC GGGCC^C
ApoI 780 GAATTT [GA]^AATT[CT]
BbrPI 1137 CACGTG CAC^GTG
BbvCI 1395 CCTCAGC CCTCAGC(-5/-2)
BglI 1443 GCCCAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmBI 1344 CGTCTC CGTCTC(1/5)
BstYI 681 AGATCC [GA]^GATC[CT]
EcoT14I 1169 CCAAGG C^C[AT][AT]GG
MflI 681 AGATCC [GA]^GATC[CT]
NheI 30 GCTAGC G^CTAGC
NspI 356 ACATGC [GA]CATG^[CT]
PmaCI 1137 CACGTG CAC^GTG
PmlI 1137 CACGTG CAC^GTG
PstI 136 CTGCAG CTGCA^G
SacI 313 GAGCTC GAGCT^C
SgrAI 38 CGCCGGCG C[GA]^CCGG[CT]G
StyI 1169 CCAAGG C^C[AT][AT]GG
TaqI 404 TCGA T^CGA
TthHB8I 404 TCGA T^CGA
XbaI 678 TCTAGA T^CTAGA
XhoII 681 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 367 GTCTAC GT^[AC][GT]AC
1411 GTCTAC
AfaI 435 GTAC GT^AC
864 GTAC
AspHI 223 GTGCTC G[AT]GC[AT]^C
313 GAGCTC
BanII 12 GGGCCC G[GA]GC[CT]^C
313 GAGCTC
BbeI 36 GGCGCC GGCGC^C
105 GGCGCC
BclI 629 TGATCA T^GATCA
800 TGATCA
BfaI 31 CTAG C^TAG
679 CTAG
BsaAI 865 TACGTG [CT]AC^GT[GA]
1137 CACGTG
BsiHKAI 223 GTGCTC G[AT]GC[AT]^C
313 GAGCTC
BsrBI 475 CCGCTC CCGCTC(-3/-3)
1276 GAGCGG
BsrFI 39 GCCGGC [GA]^CCGG[CT]
108 GCCGGC
BsrI 711 ACTGG ACTGG(1/-1)
1211 ACTGG
BssSI 517 CTCGTG CACGAG(-5/-1)
1129 CTCGTG
Cfr10I 39 GCCGGC [GA]^CCGG[CT]
108 GCCGGC
DdeI 1268 CTGAG C^T[ACGT]AG
1396 CTCAG
DraII 1021 GGGTCCC [GA]G^G[ACGT]CC[CT]
1057 GGGTCCC
EagI 44 CGGCCG C^GGCCG
548 CGGCCG
EclXI 44 CGGCCG C^GGCCG
548 CGGCCG
Eco52I 44 CGGCCG C^GGCCG
548 CGGCCG
EcoO109I 1021 GGGTCCC [GA]G^G[ACGT]CC[CT]
1057 GGGTCCC
FbaI 629 TGATCA T^GATCA
800 TGATCA
KasI 36 GGCGCC G^GCGCC
105 GGCGCC
MaeI 31 CTAG C^TAG
679 CTAG
MaeII 866 ACGT A^CGT
1138 ACGT
NaeI 39 GCCGGC GCC^GGC
108 GCCGGC
NarI 36 GGCGCC GG^CGCC
105 GGCGCC
NgoMIV 39 GCCGGC G^CCGGC
108 GCCGGC
PpuMI 1021 GGGTCCC [GA]G^G[AT]CC[CT]
1057 GGGTCCC
RsaI 435 GTAC GT^AC
864 GTAC
SapI 477 GCTCTTC GCTCTTC(1/4)
1044 GCTCTTC
SfoI 36 GGCGCC GGC^GCC
105 GGCGCC
SmaI 581 CCCGGG CCC^GGG
1061 CCCGGG
Tsp509I 772 AATT ^AATT
781 AATT
XcmI 707 CCAAACTGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
848 CCATCGTGACGCTGG
XmaCI 581 CCCGGG C^CCGGG
1061 CCCGGG
XmaI 581 CCCGGG C^CCGGG
1061 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AvaI AvaII BalI BanI BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsiEI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BssHII BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DpnII DsaI EaeI EarI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TseI Tsp45I