Restriction map for
pFN21AE4478
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AvaI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BclI BcnI BfrI
BglI BlnI BpuAI BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsoBI
BspDI BspEI BspLU11I BsrBI BsrDI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoT22I FbaI FseI FspI
HaeII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI NarI NciI NcoI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 930 CGCG CG^CG
AcyI 1435 GGCGTC G[GA]^CG[CT]C
AfaI 420 GTAC GT^AC
AhdI 245 GACTCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1185 GGGCCC GGGCC^C
AspEI 245 GACTCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1432 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 200 TGGCCA TGG^CCA
BciVI 703 GTATCC GTATCC(6/5)
BlpI 664 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 664 GCTAAGC GC^T[ACGT]AGC
BsaHI 1435 GGCGTC G[GA]^CG[CT]C
BseRI 655 GAGGAG GAGGAG(10/8)
BsmBI 179 CGTCTC CGTCTC(1/5)
BsmI 660 GAATGC GAATGC(1/-1)
Bsp1407I 419 TGTACA T^GTACA
BspHI 1043 TCATGA T^CATGA
BspMI 895 GCAGGT ACCTGC(4/8)
BsrGI 419 TGTACA T^GTACA
BstAPI 1405 GCACACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 15 GGTCACC G^GT[ACGT]ACC
BstPI 15 GGTCACC G^GT[ACGT]ACC
BstUI 930 CGCG CG^CG
BstXI 507 CCAGATCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 664 GCTAAGC GC^T[ACGT]AGC
DraIII 1406 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1359 CCGTGG C^C[GA][CT]GG
Eam1105I 245 GACTCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 15 GGTCACC G^GT[ACGT]ACC
EcoRV 1378 GATATC GAT^ATC
HgaI 1436 GCGTC GACGC(5/10)
Hin1I 1435 GGCGTC G[GA]^CG[CT]C
MluNI 200 TGGCCA TGG^CCA
MscI 200 TGGCCA TGG^CCA
MvnI 930 CGCG CG^CG
NaeI 1213 GCCGGC GCC^GGC
NdeI 1003 CATATG CA^TATG
NgoMIV 1213 GCCGGC G^CCGGC
PflFI 1432 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1148 CCATTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1043 TCATGA T^CATGA
RsaI 420 GTAC GT^AC
SacI 775 GAGCTC GAGCT^C
SfcI 956 CTGTAG C^T[GA][CT]AG
SspBI 419 TGTACA T^GTACA
StuI 1011 AGGCCT AGG^CCT
TaqI 1457 TCGA T^CGA
TfiI 881 GAATC G^A[AT]TC
Tth111I 1432 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1457 TCGA T^CGA
Van91I 1148 CCATTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AspHI 775 GAGCTC G[AT]GC[AT]^C
823 GTGCTC
BanI 454 GGTGCC G^G[CT][GA]CC
691 GGCACC
BfaI 772 CTAG C^TAG
1243 CTAG
BpmI 676 CTCCAG CTGGAG(16/14)
826 CTCCAG
BsaI 1354 GAGACC GGTCTC(1/5)
1471 GAGACC
BsiHKAI 775 GAGCTC G[AT]GC[AT]^C
823 GTGCTC
BsrFI 1213 GCCGGC [GA]^CCGG[CT]
1306 ACCGGC
CfoI 270 GCGC GCG^C
929 GCGC
Cfr10I 1213 GCCGGC [GA]^CCGG[CT]
1306 ACCGGC
EcoRI 494 GAATTC G^AATTC
523 GAATTC
EcoT14I 531 CCTTGG C^C[AT][AT]GG
1014 CCTTGG
HapII 1214 CCGG C^CGG
1307 CCGG
HhaI 270 GCGC GCG^C
929 GCGC
HinP1I 270 GCGC G^CGC
929 GCGC
HincII 253 GTCAAC GT[CT]^[GA]AC
289 GTCAAC
HindII 253 GTCAAC GT[CT]^[GA]AC
289 GTCAAC
HpaII 1214 CCGG C^CGG
1307 CCGG
MaeI 772 CTAG C^TAG
1243 CTAG
MaeII 178 ACGT A^CGT
1289 ACGT
MslI 1038 CACGCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1440 CATGGAGATG
MspI 1214 CCGG C^CGG
1307 CCGG
PvuII 475 CAGCTG CAG^CTG
1196 CAGCTG
SapI 541 GAAGAGC GCTCTTC(1/4)
1254 GCTCTTC
StyI 531 CCTTGG C^C[AT][AT]GG
1014 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BanII BbvI BglII BmyI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeIII
MboI MboII MflI MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfaNI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII