Restriction map for
pFN21AE4453
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# NO CUT ENZYMES ( 124 enzymes) #
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AccI AclI AcsI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AvaI AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BlnI
BsaAI BsiEI BsiWI BsoBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstXI
BstZ17I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FbaI FseI HaeII HincII HindII
HindIII HinfI HpaI KasI KspI
MaeI MfeI MluI MseI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 655 GACGTC GACGT^C
Acc65I 455 GGTACC G^GTACC
AccIII 1007 TCCGGA T^CCGGA
AcyI 655 GACGTC G[GA]^CG[CT]C
Asp718I 455 GGTACC G^GTACC
AviII 555 TGCGCA TGC^GCA
BanI 455 GGTACC G^G[CT][GA]CC
BglI 583 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1202 AGATCT A^GATCT
BlpI 1050 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1050 GCTCAGC GC^T[ACGT]AGC
BsaBI 714 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 655 GACGTC G[GA]^CG[CT]C
BsaWI 1007 TCCGGA [AT]^CCGG[AT]
BseAI 1007 TCCGGA T^CCGGA
BsgI 827 CTGCAC GTGCAG(16/14)
BsmBI 905 GAGACG CGTCTC(1/5)
BsmI 552 GAATGC GAATGC(1/-1)
BspEI 1007 TCCGGA T^CCGGA
BspMI 7 ACCTGC ACCTGC(4/8)
BsrDI 534 CATTGC GCAATG(2/0)
BsrFI 796 ACCGGC [GA]^CCGG[CT]
BssSI 763 CACGAG CACGAG(-5/-1)
BstEII 354 GGTGACC G^GT[ACGT]ACC
BstPI 354 GGTGACC G^GT[ACGT]ACC
CelII 1050 GCTCAGC GC^T[ACGT]AGC
Cfr10I 796 ACCGGC [GA]^CCGG[CT]
EarI 1347 CTCTTC CTCTTC(1/4)
EcoO65I 354 GGTGACC G^GT[ACGT]ACC
EcoT14I 267 CCTTGG C^C[AT][AT]GG
FspI 555 TGCGCA TGC^GCA
Hin1I 655 GACGTC G[GA]^CG[CT]C
KpnI 455 GGTACC GGTAC^C
Ksp632I 1347 CTCTTC CTCTTC(1/4)
MamI 714 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1007 TCCGGA T^CCGGA
NspI 1106 GCATGC [GA]CATG^[CT]
PflMI 1020 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 487 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 916 ACCTGGT A^CC[AT]GGT
SmlI 1375 CTCAAG C^T[CT][GA]AG
SphI 1106 GCATGC GCATG^C
StuI 726 AGGCCT AGG^CCT
StyI 267 CCTTGG C^C[AT][AT]GG
Van91I 1020 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccII 1223 CGCG CG^CG
1321 CGCG
AlwI 444 GATCC GGATC(4/5)
1122 GATCC
AlwNI 1021 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1126 CAGGGACTG
AocI 519 CCTGAGG CC^T[ACGT]AGG
750 CCTGAGG
BalI 695 TGGCCA TGG^CCA
1302 TGGCCA
BbsI 1218 GAAGAC GAAGAC(2/6)
1327 GTCTTC
BcnI 672 CCGGG CC^[GC]GG
1364 CCCGG
BpuAI 1218 GAAGAC GAAGAC(2/6)
1327 GTCTTC
BsaI 913 GAGACC GGTCTC(1/5)
1087 GAGACC
BstUI 1223 CGCG CG^CG
1321 CGCG
Bsu36I 519 CCTGAGG CC^T[ACGT]AGG
750 CCTGAGG
CfoI 556 GCGC GCG^C
669 GCGC
Eco81I 519 CCTGAGG CC^T[ACGT]AGG
750 CCTGAGG
FauI 1319 CCCGC CCCGC(4/6)
185 GCGGG
HgaI 907 GACGC GACGC(5/10)
1221 GACGC
HhaI 556 GCGC GCG^C
669 GCGC
HinP1I 556 GCGC G^CGC
669 GCGC
MaeII 656 ACGT A^CGT
812 ACGT
MluNI 695 TGGCCA TGG^CCA
1302 TGGCCA
MscI 695 TGGCCA TGG^CCA
1302 TGGCCA
MvnI 1223 CGCG CG^CG
1321 CGCG
NciI 672 CCGGG CC^[GC]GG
1364 CCCGG
PpuMI 112 GGGTCCT [GA]G^G[AT]CC[CT]
515 AGGACCT
PstI 9 CTGCAG CTGCA^G
1039 CTGCAG
PvuII 1111 CAGCTG CAG^CTG
1153 CAGCTG
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
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AciI AfaI AluI AspHI AvaII
BanII BbvI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EaeI EcoNI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TseI Tsp45I
TspRI TthHB8I XcmI XhoII