Restriction map for
pFN21AE6326
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AcyI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BspDI
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI FspI HaeII Hin1I HincII
HindII HpaI KasI KpnI KspI
MamI MluNI MscI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AclI 1073 AACGTT AA^CGTT
AlwI 336 GGATC GGATC(4/5)
AlwNI 518 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 1031 CCCGAG C^[CT]CG[GA]G
AvaII 23 GGTCC G^G[AT]CC
BmyI 812 GTGCCC G[AGT]GC[ACT]^C
BseRI 364 GAGGAG GAGGAG(10/8)
BsmAI 1052 GTCTC GTCTC(1/5)
BsoBI 1031 CCCGAG C^[CT]CG[GA]G
Bsp1286I 812 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 1149 TGTACA T^GTACA
BspLU11I 1175 ACATGT A^CATGT
BsrDI 511 GCAATG GCAATG(2/0)
BsrGI 1149 TGTACA T^GTACA
BsrI 581 ACTGG ACTGG(1/-1)
BstBI 146 TTCGAA TT^CGAA
BstYI 336 GGATCT [GA]^GATC[CT]
DpnII 337 GATC ^GATC
DraII 22 GGGTCCC [GA]G^G[ACGT]CC[CT]
EarI 826 GAAGAG CTCTTC(1/4)
EcoO109I 22 GGGTCCC [GA]G^G[ACGT]CC[CT]
FauI 67 CCCGC CCCGC(4/6)
HgaI 32 GCGTC GACGC(5/10)
HphI 975 TCACC GGTGA(8/7)
Ksp632I 826 GAAGAG CTCTTC(1/4)
MboI 337 GATC ^GATC
MfeI 1190 CAATTG C^AATTG
MflI 336 GGATCT [GA]^GATC[CT]
MluI 30 ACGCGT A^CGCGT
MunI 1190 CAATTG C^AATTG
NdeII 337 GATC ^GATC
NspI 1175 ACATGT [GA]CATG^[CT]
NspV 146 TTCGAA TT^CGAA
PflMI 1225 CCAATCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1081 GACTC GAGTC(4/5)
PpuMI 22 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1045 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1073 AACGTT AA^CGTT
PstI 485 CTGCAG CTGCA^G
Sau3AI 337 GATC ^GATC
SfuI 146 TTCGAA TT^CGAA
SspBI 1149 TGTACA T^GTACA
TaqI 147 TCGA T^CGA
TthHB8I 147 TCGA T^CGA
Van91I 1225 CCAATCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 105 TCTAGA T^CTAGA
XcmI 1088 CCACTGCTTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 336 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 88 TCCGGA T^CCGGA
179 TCCGGA
AcsI 143 GAATTC [GA]^AATT[CT]
910 GAATTC
AflIII 30 ACGCGT A^C[GA][CT]GT
1175 ACATGT
ApoI 143 GAATTC [GA]^AATT[CT]
910 GAATTC
BciVI 768 GGATAC GTATCC(6/5)
1171 GGATAC
BsaWI 88 TCCGGA [AT]^CCGG[AT]
179 TCCGGA
BseAI 88 TCCGGA T^CCGGA
179 TCCGGA
BsmFI 732 GGGAC GGGAC(10/14)
24 GTCCC
BspEI 88 TCCGGA T^CCGGA
179 TCCGGA
BspHI 660 TCATGA T^CATGA
851 TCATGA
BstF5I 570 GGATG GGATG(2/0)
1209 GGATG
Cfr13I 23 GGTCC G^G[ACGT]CC
391 GGCCC
EaeI 2 TGGCCG [CT]^GGCC[GA]
42 CGGCCA
EcoRI 143 GAATTC G^AATTC
910 GAATTC
FokI 570 GGATG GGATG(9/13)
1209 GGATG
HapII 89 CCGG C^CGG
180 CCGG
HpaII 89 CCGG C^CGG
180 CCGG
MaeII 102 ACGT A^CGT
1074 ACGT
MroI 88 TCCGGA T^CCGGA
179 TCCGGA
MspA1I 61 CAGCTG C[AC]G^C[GT]G
1057 CAGCTG
MspI 89 CCGG C^CGG
180 CCGG
NlaIV 22 GGGTCC GG[ACGT]^[ACGT]CC
23 GGTCCC
PvuII 61 CAGCTG CAG^CTG
1057 CAGCTG
RcaI 660 TCATGA T^CATGA
851 TCATGA
Sau96I 23 GGTCC G^G[ACGT]CC
391 GGCCC
SfcI 485 CTGCAG C^T[GA][CT]AG
979 CTACAG
SspI 304 AATATT AAT^ATT
1106 AATATT
TspRI 986 CAGTG CA[GC]TG[ACGT][ACGT]^
1089 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AfaI AluI Asp700I
BbvI BfaI BpmI BsaJI BsiYI
BslI BssKI BstNI BstUI Cac8I
CfoI DdeI DsaI EcoRII EcoT14I
Fnu4HI HaeIII HhaI HinP1I HindIII
HinfI ItaI MaeI MaeIII MboII
MnlI MseI MslI MvaI MvnI
MwoI NcoI NlaIII RsaI ScrFI
SfaNI StyI TfiI Tru9I TseI
Tsp45I Tsp509I XmnI