Restriction map for
pFN21AE0991
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII AclI AcsI AcyI AflII
AflIII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI AspEI AspI
AviII AvrII BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BsaAI BsaBI BsaHI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HindIII HpaI KasI KspI
MaeII MamI MfeI MluI MseI
MslI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfaNI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tth111I XbaI XcmI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 491 GGTACC G^GTACC
AccI 211 GTCTAC GT^[AC][GT]AC
AccIII 1263 TCCGGA T^CCGGA
Alw44I 358 GTGCAC G^TGCAC
ApaLI 358 GTGCAC G^TGCAC
Asp700I 601 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 491 GGTACC G^GTACC
BamHI 938 GGATCC G^GATCC
BbsI 1153 GAAGAC GAAGAC(2/6)
BlpI 420 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 420 GCTCAGC GC^T[ACGT]AGC
BpuAI 1153 GAAGAC GAAGAC(2/6)
BsaI 1267 GAGACC GGTCTC(1/5)
BsaWI 1263 TCCGGA [AT]^CCGG[AT]
BseAI 1263 TCCGGA T^CCGGA
BspEI 1263 TCCGGA T^CCGGA
BsrDI 474 CATTGC GCAATG(2/0)
BstAPI 1614 GCATAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1254 GGTGACC G^GT[ACGT]ACC
BstPI 1254 GGTGACC G^GT[ACGT]ACC
BstXI 1576 CCACAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 420 GCTCAGC GC^T[ACGT]AGC
DsaI 647 CCGTGG C^C[GA][CT]GG
EcoNI 1522 CCTAGATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1254 GGTGACC G^GT[ACGT]ACC
EcoT14I 1250 CCTTGG C^C[AT][AT]GG
HincII 1132 GTCAAC GT[CT]^[GA]AC
HindII 1132 GTCAAC GT[CT]^[GA]AC
KpnI 491 GGTACC GGTAC^C
MroI 1263 TCCGGA T^CCGGA
NaeI 193 GCCGGC GCC^GGC
NgoMIV 193 GCCGGC G^CCGGC
PaeR7I 1127 CTCGAG C^TCGAG
SfiI 65 GGCCCCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1127 CTCGAG C^T[CT][GA]AG
StuI 483 AGGCCT AGG^CCT
StyI 1250 CCTTGG C^C[AT][AT]GG
Tsp509I 918 AATT ^AATT
XhoI 1127 CTCGAG C^TCGAG
XmnI 601 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 492 GTAC GT^AC
760 GTAC
AocI 336 CCTAAGG CC^T[ACGT]AGG
1158 CCTCAGG
BalI 866 TGGCCA TGG^CCA
1469 TGGCCA
BglI 66 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
447 GCCGGTCAGGC
BseRI 114 CTCCTC GAGGAG(10/8)
1810 CTCCTC
BsrBI 1305 CCGCTC CCGCTC(-3/-3)
1642 GAGCGG
BstYI 938 GGATCC [GA]^GATC[CT]
1044 AGATCC
Bsu36I 336 CCTAAGG CC^T[ACGT]AGG
1158 CCTCAGG
EarI 1075 CTCTTC CTCTTC(1/4)
1393 GAAGAG
Eco81I 336 CCTAAGG CC^T[ACGT]AGG
1158 CCTCAGG
HaeII 136 AGCGCC [GA]GCGC^[CT]
1446 AGCGCC
Ksp632I 1075 CTCTTC CTCTTC(1/4)
1393 GAAGAG
MflI 938 GGATCC [GA]^GATC[CT]
1044 AGATCC
MluNI 866 TGGCCA TGG^CCA
1469 TGGCCA
MscI 866 TGGCCA TGG^CCA
1469 TGGCCA
PflMI 924 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1357 CCAGGTCCTGG
PshAI 1125 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1269 GACCCTGGTC
PstI 764 CTGCAG CTGCA^G
1855 CTGCAG
RsaI 492 GTAC GT^AC
760 GTAC
SacI 745 GAGCTC GAGCT^C
1327 GAGCTC
SfcI 764 CTGCAG C^T[GA][CT]AG
1855 CTGCAG
TaqI 561 TCGA T^CGA
1128 TCGA
TfiI 606 GATTC G^A[AT]TC
1096 GAATC
Tsp45I 584 GTGAC ^GT[GC]AC
1255 GTGAC
TthHB8I 561 TCGA T^CGA
1128 TCGA
Van91I 924 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1357 CCAGGTCCTGG
XhoII 938 GGATCC [GA]^GATC[CT]
1044 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PvuII
Sau3AI Sau96I ScrFI SmaI TseI
TspRI XmaCI XmaI