Restriction map for
pFN21AE1383
##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspI
AviII AvrII BamHI BbeI BbvCI
BciVI BfaI BfrI BlnI BsaBI
BsiWI BsmI Bsp1407I BspDI BsrDI
BsrGI Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoRV FseI
FspI HaeII HindIII HpaI KasI
MaeI MamI MfeI MseI MunI
NarI NdeI NheI NotI NruI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI SbfI ScaI SexAI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 191 GGTACC G^GTACC
AccI 940 GTCTAC GT^[AC][GT]AC
Asp718I 191 GGTACC G^GTACC
BbrPI 988 CACGTG CAC^GTG
BclI 1503 TGATCA T^GATCA
BglI 64 GCCACGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 988 CACGTG [CT]AC^GT[GA]
BsaI 697 GAGACC GGTCTC(1/5)
BspLU11I 1691 ACATGT A^CATGT
BssSI 1418 CACGAG CACGAG(-5/-1)
BstEII 156 GGTGACC G^GT[ACGT]ACC
BstPI 156 GGTGACC G^GT[ACGT]ACC
BstXI 236 CCAGGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoO65I 156 GGTGACC G^GT[ACGT]ACC
EcoRI 250 GAATTC G^AATTC
EcoT22I 1681 ATGCAT ATGCA^T
FbaI 1503 TGATCA T^GATCA
HincII 1297 GTCAAC GT[CT]^[GA]AC
HindII 1297 GTCAAC GT[CT]^[GA]AC
KpnI 191 GGTACC GGTAC^C
KspI 1872 CCGCGG CCGC^GG
MluI 776 ACGCGT A^CGCGT
MslI 678 CATCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 69 GCCGGC GCC^GGC
NgoMIV 69 GCCGGC G^CCGGC
NsiI 1681 ATGCAT ATGCA^T
PaeR7I 1762 CTCGAG C^TCGAG
PmaCI 988 CACGTG CAC^GTG
PmlI 988 CACGTG CAC^GTG
SacII 1872 CCGCGG CCGC^GG
SfiI 63 GGCCACGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1136 GCATGC GCATG^C
TfiI 1484 GAATC G^A[AT]TC
XhoI 1762 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 426 TCCGGA T^CCGGA
1074 TCCGGA
AcsI 250 GAATTC [GA]^AATT[CT]
490 AAATTC
AcyI 1441 GACGCC G[GA]^CG[CT]C
1945 GACGCC
ApoI 250 GAATTC [GA]^AATT[CT]
490 AAATTC
Asp700I 564 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1881 GAAACAGTTC
AvaI 103 CCCGAG C^[CT]CG[GA]G
1762 CTCGAG
BalI 44 TGGCCA TGG^CCA
984 TGGCCA
BcgI 205 CGAGACTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1104 CGACAGCAGTGC
BglII 170 AGATCT A^GATCT
320 AGATCT
BlpI 798 GCTCAGC GC^T[ACGT]AGC
2022 GCTGAGC
Bpu1102I 798 GCTCAGC GC^T[ACGT]AGC
2022 GCTGAGC
BsaHI 1441 GACGCC G[GA]^CG[CT]C
1945 GACGCC
BseAI 426 TCCGGA T^CCGGA
1074 TCCGGA
BsmBI 286 GAGACG CGTCTC(1/5)
1831 GAGACG
BsoBI 103 CCCGAG C^[CT]CG[GA]G
1762 CTCGAG
BspEI 426 TCCGGA T^CCGGA
1074 TCCGGA
BspHI 338 TCATGA T^CATGA
818 TCATGA
BsrBI 1359 CCGCTC CCGCTC(-3/-3)
1543 GAGCGG
BsrFI 69 GCCGGC [GA]^CCGG[CT]
1601 ACCGGC
BssHII 76 GCGCGC G^CGCGC
78 GCGCGC
CelII 798 GCTCAGC GC^T[ACGT]AGC
2022 GCTGAGC
Cfr10I 69 GCCGGC [GA]^CCGG[CT]
1601 ACCGGC
Hin1I 1441 GACGCC G[GA]^CG[CT]C
1945 GACGCC
MluNI 44 TGGCCA TGG^CCA
984 TGGCCA
MroI 426 TCCGGA T^CCGGA
1074 TCCGGA
MscI 44 TGGCCA TGG^CCA
984 TGGCCA
NcoI 47 CCATGG C^CATGG
1805 CCATGG
PleI 328 GACTC GAGTC(4/5)
1243 GACTC
PstI 1084 CTGCAG CTGCA^G
1285 CTGCAG
PvuII 1636 CAGCTG CAG^CTG
1966 CAGCTG
RcaI 338 TCATGA T^CATGA
818 TCATGA
SacI 541 GAGCTC GAGCT^C
1510 GAGCTC
SfcI 1084 CTGCAG C^T[GA][CT]AG
1285 CTGCAG
SmlI 1762 CTCGAG C^T[CT][GA]AG
1864 CTCAAG
Tsp45I 157 GTGAC ^GT[GC]AC
1019 GTGAC
Tsp509I 251 AATT ^AATT
491 AATT
XmnI 564 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1881 GAAACAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AspHI AvaII BanI
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PpuMI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TseI TspRI TthHB8I Van91I
XcmI XhoII