Restriction map for
pFN21AE1472
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AvaI
AvaII AviII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfrI BglI BglII BpmI
BsaBI BsaHI BsaI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI BstXI Bsu36I Cfr10I ClaI
CpoI DraII DraIII DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
FauI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MluI MluNI
MroI MscI MspA1I MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SphI Sse8387I SspBI StuI
SwaI TfiI XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AfaI 686 GTAC GT^AC
AseI 657 ATTAAT AT^TAAT
AsnI 657 ATTAAT AT^TAAT
Asp700I 421 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 209 GAGCTC G[AT]GC[AT]^C
AvrII 904 CCTAGG C^CTAGG
BbsI 888 GAAGAC GAAGAC(2/6)
BbvI 41 GCTGC GCAGC(8/12)
BclI 554 TGATCA T^GATCA
BlnI 904 CCTAGG C^CTAGG
BlpI 52 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 52 GCTCAGC GC^T[ACGT]AGC
BpuAI 888 GAAGAC GAAGAC(2/6)
BsaAI 163 TACGTA [CT]AC^GT[GA]
BsaWI 505 TCCGGT [AT]^CCGG[AT]
BsiHKAI 209 GAGCTC G[AT]GC[AT]^C
BsmI 615 GAATGC GAATGC(1/-1)
BsrDI 714 CATTGC GCAATG(2/0)
BsrI 622 ACTGG ACTGG(1/-1)
BstF5I 516 GGATG GGATG(2/0)
BstYI 502 AGATCC [GA]^GATC[CT]
Cac8I 37 GCGTGC GC[ACGT]^[ACGT]GC
CelII 52 GCTCAGC GC^T[ACGT]AGC
CfoI 19 GCGC GCG^C
DraI 178 TTTAAA TTT^AAA
DrdI 103 GACACTGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 85 GAATTC G^AATTC
EcoT22I 796 ATGCAT ATGCA^T
FbaI 554 TGATCA T^GATCA
FokI 516 GGATG GGATG(9/13)
HaeII 18 GGCGCT [GA]GCGC^[CT]
HapII 506 CCGG C^CGG
HhaI 19 GCGC GCG^C
HinP1I 19 GCGC G^CGC
HinfI 768 GAGTC G^A[ACGT]TC
HpaII 506 CCGG C^CGG
MfeI 527 CAATTG C^AATTG
MflI 502 AGATCC [GA]^GATC[CT]
MslI 670 CACTACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 506 CCGG C^CGG
MunI 527 CAATTG C^AATTG
MwoI 32 GCTCGGCGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 796 ATGCAT ATGCA^T
PflMI 793 CCAATGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 768 GAGTC GAGTC(4/5)
PshBI 657 ATTAAT AT^TAAT
RsaI 686 GTAC GT^AC
SacI 209 GAGCTC GAGCT^C
SnaBI 163 TACGTA TAC^GTA
TaqI 380 TCGA T^CGA
TseI 41 GCTGC G^C[AT]GC
Tsp45I 640 GTGAC ^GT[GC]AC
TthHB8I 380 TCGA T^CGA
Van91I 793 CCAATGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 502 AGATCC [GA]^GATC[CT]
XmnI 421 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 160 GTATAC GT^[AC][GT]AC
788 GTATAC
AlwI 503 GATCC GGATC(4/5)
46 GGATC
AspI 103 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
764 GACTGAGTC
BanII 30 GGGCTC G[GA]GC[CT]^C
209 GAGCTC
BmyI 30 GGGCTC G[AGT]GC[ACT]^C
209 GAGCTC
Bsp1286I 30 GGGCTC G[AGT]GC[ACT]^C
209 GAGCTC
BssKI 9 CCTGG ^CC[ACGT]GG
595 CCAGG
Bst1107I 160 GTATAC GTA^TAC
788 GTATAC
BstNI 9 CCTGG CC^[AT]GG
595 CCAGG
BstZ17I 160 GTATAC GTA^TAC
788 GTATAC
Cfr13I 5 GGCCC G^G[ACGT]CC
802 GGCCC
EarI 806 CTCTTC CTCTTC(1/4)
185 GAAGAG
EcoRII 9 CCTGG ^CC[AT]GG
595 CCAGG
EcoT14I 627 CCTTGG C^C[AT][AT]GG
904 CCTAGG
Ksp632I 806 CTCTTC CTCTTC(1/4)
185 GAAGAG
MaeII 91 ACGT A^CGT
164 ACGT
MvaI 9 CCTGG CC^[AT]GG
595 CCAGG
NlaIV 5 GGCCCC GG[ACGT]^[ACGT]CC
577 GGAGCC
PflFI 103 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
764 GACTGAGTC
Sau96I 5 GGCCC G^G[ACGT]CC
802 GGCCC
ScrFI 9 CCTGG CC^[ACGT]GG
595 CCAGG
SfaNI 283 GATGC GCATC(5/9)
310 GATGC
SfcI 80 CTGTAG C^T[GA][CT]AG
647 CTACAG
SpeI 549 ACTAGT A^CTAGT
855 ACTAGT
StyI 627 CCTTGG C^C[AT][AT]GG
904 CCTAGG
Tth111I 103 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
764 GACTGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AcsI AluI ApoI BfaI
BsaJI BsiYI BslI BsmAI BsmFI
DdeI DpnII Fnu4HI HaeIII HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI NdeII NlaIII Sau3AI
SspI Tru9I Tsp509I TspRI