Restriction map for
pFN21AE6713
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AhdI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BbvCI
BcgI BciVI BfrI BglII BlnI
BsaBI BsaWI BseAI BseRI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRV EcoT22I FspI HpaI
KspI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmeI PshAI PshBI
Psp1406I RcaI RsrII SacII SalI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 331 GACGTC GACGT^C
AflIII 162 ACGTGT A^C[GA][CT]GT
Alw44I 1234 GTGCAC G^TGCAC
AocI 1599 CCTCAGG CC^T[ACGT]AGG
Aor51HI 977 AGCGCT AGC^GCT
ApaLI 1234 GTGCAC G^TGCAC
BamHI 506 GGATCC G^GATCC
BbeI 14 GGCGCC GGCGC^C
BbrPI 161 CACGTG CAC^GTG
BclI 899 TGATCA T^GATCA
BfaI 919 CTAG C^TAG
BsiEI 674 CGATCG CG[GA][CT]^CG
BspMI 1106 ACCTGC ACCTGC(4/8)
BsrDI 1830 GCAATG GCAATG(2/0)
BstYI 506 GGATCC [GA]^GATC[CT]
Bsu36I 1599 CCTCAGG CC^T[ACGT]AGG
DrdI 601 GACACGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 977 AGCGCT AGC^GCT
Eco81I 1599 CCTCAGG CC^T[ACGT]AGG
EcoNI 2152 CCTGCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 899 TGATCA T^GATCA
FseI 219 GGCCGGCC GGCCGG^CC
HincII 1501 GTCAAC GT[CT]^[GA]AC
HindII 1501 GTCAAC GT[CT]^[GA]AC
HindIII 1033 AAGCTT A^AGCTT
HinfI 68 GATTC G^A[ACGT]TC
KasI 14 GGCGCC G^GCGCC
MaeI 919 CTAG C^TAG
MflI 506 GGATCC [GA]^GATC[CT]
MseI 761 TTAA T^TAA
NarI 14 GGCGCC GG^CGCC
PflMI 1926 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 161 CACGTG CAC^GTG
PmlI 161 CACGTG CAC^GTG
PpuMI 86 GGGACCT [GA]G^G[AT]CC[CT]
PvuI 674 CGATCG CGAT^CG
SexAI 1149 ACCTGGT A^CC[AT]GGT
SfoI 14 GGCGCC GGC^GCC
TfiI 68 GATTC G^A[AT]TC
Tru9I 761 TTAA T^TAA
Van91I 1926 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 506 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 704 GGTACC G^GTACC
1754 GGTACC
AccII 39 CGCG CG^CG
586 CGCG
AcsI 1060 GAATTC [GA]^AATT[CT]
2074 GAATTC
ApoI 1060 GAATTC [GA]^AATT[CT]
2074 GAATTC
Asp718I 704 GGTACC G^GTACC
1754 GGTACC
BalI 296 TGGCCA TGG^CCA
407 TGGCCA
BbsI 1195 GAAGAC GAAGAC(2/6)
1549 GTCTTC
BglI 6 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
111 GCCTGGCGGGC
BpuAI 1195 GAAGAC GAAGAC(2/6)
1549 GTCTTC
BsaAI 161 CACGTG [CT]AC^GT[GA]
1855 TACGTG
BsmBI 329 GAGACG CGTCTC(1/5)
424 GAGACG
BssSI 1759 CACGAG CACGAG(-5/-1)
1868 CACGAG
BstUI 39 CGCG CG^CG
586 CGCG
BstXI 863 CCACCTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1367 CCATGAGCCTGG
EarI 358 CTCTTC CTCTTC(1/4)
430 CTCTTC
EcoRI 1060 GAATTC G^AATTC
2074 GAATTC
KpnI 704 GGTACC GGTAC^C
1754 GGTACC
Ksp632I 358 CTCTTC CTCTTC(1/4)
430 CTCTTC
MluNI 296 TGGCCA TGG^CCA
407 TGGCCA
MscI 296 TGGCCA TGG^CCA
407 TGGCCA
MvnI 39 CGCG CG^CG
586 CGCG
NcoI 1316 CCATGG C^CATGG
2100 CCATGG
PstI 1327 CTGCAG CTGCA^G
2134 CTGCAG
SapI 357 GCTCTTC GCTCTTC(1/4)
429 GCTCTTC
StuI 1427 AGGCCT AGG^CCT
1976 AGGCCT
TaqI 203 TCGA T^CGA
1424 TCGA
TthHB8I 203 TCGA T^CGA
1424 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbvI BcnI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TseI Tsp45I Tsp509I TspRI
XcmI XmaCI XmaI