################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AccI AccIII AclI AflII AgeI AhdI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbvCI BcgI BciVI BfrI BglII BlnI BsaBI BsaWI BseAI BseRI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoO65I EcoRV EcoT22I FspI HpaI KspI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmeI PshAI PshBI Psp1406I RcaI RsrII SacII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 331 GACGTC GACGT^C AflIII 162 ACGTGT A^C[GA][CT]GT Alw44I 1234 GTGCAC G^TGCAC AocI 1599 CCTCAGG CC^T[ACGT]AGG Aor51HI 977 AGCGCT AGC^GCT ApaLI 1234 GTGCAC G^TGCAC BamHI 506 GGATCC G^GATCC BbeI 14 GGCGCC GGCGC^C BbrPI 161 CACGTG CAC^GTG BclI 899 TGATCA T^GATCA BfaI 919 CTAG C^TAG BsiEI 674 CGATCG CG[GA][CT]^CG BspMI 1106 ACCTGC ACCTGC(4/8) BsrDI 1830 GCAATG GCAATG(2/0) BstYI 506 GGATCC [GA]^GATC[CT] Bsu36I 1599 CCTCAGG CC^T[ACGT]AGG DrdI 601 GACACGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 977 AGCGCT AGC^GCT Eco81I 1599 CCTCAGG CC^T[ACGT]AGG EcoNI 2152 CCTGCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 899 TGATCA T^GATCA FseI 219 GGCCGGCC GGCCGG^CC HincII 1501 GTCAAC GT[CT]^[GA]AC HindII 1501 GTCAAC GT[CT]^[GA]AC HindIII 1033 AAGCTT A^AGCTT HinfI 68 GATTC G^A[ACGT]TC KasI 14 GGCGCC G^GCGCC MaeI 919 CTAG C^TAG MflI 506 GGATCC [GA]^GATC[CT] MseI 761 TTAA T^TAA NarI 14 GGCGCC GG^CGCC PflMI 1926 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 161 CACGTG CAC^GTG PmlI 161 CACGTG CAC^GTG PpuMI 86 GGGACCT [GA]G^G[AT]CC[CT] PvuI 674 CGATCG CGAT^CG SexAI 1149 ACCTGGT A^CC[AT]GGT SfoI 14 GGCGCC GGC^GCC TfiI 68 GATTC G^A[AT]TC Tru9I 761 TTAA T^TAA Van91I 1926 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 506 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Acc65I 704 GGTACC G^GTACC 1754 GGTACC AccII 39 CGCG CG^CG 586 CGCG AcsI 1060 GAATTC [GA]^AATT[CT] 2074 GAATTC ApoI 1060 GAATTC [GA]^AATT[CT] 2074 GAATTC Asp718I 704 GGTACC G^GTACC 1754 GGTACC BalI 296 TGGCCA TGG^CCA 407 TGGCCA BbsI 1195 GAAGAC GAAGAC(2/6) 1549 GTCTTC BglI 6 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 111 GCCTGGCGGGC BpuAI 1195 GAAGAC GAAGAC(2/6) 1549 GTCTTC BsaAI 161 CACGTG [CT]AC^GT[GA] 1855 TACGTG BsmBI 329 GAGACG CGTCTC(1/5) 424 GAGACG BssSI 1759 CACGAG CACGAG(-5/-1) 1868 CACGAG BstUI 39 CGCG CG^CG 586 CGCG BstXI 863 CCACCTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1367 CCATGAGCCTGG EarI 358 CTCTTC CTCTTC(1/4) 430 CTCTTC EcoRI 1060 GAATTC G^AATTC 2074 GAATTC KpnI 704 GGTACC GGTAC^C 1754 GGTACC Ksp632I 358 CTCTTC CTCTTC(1/4) 430 CTCTTC MluNI 296 TGGCCA TGG^CCA 407 TGGCCA MscI 296 TGGCCA TGG^CCA 407 TGGCCA MvnI 39 CGCG CG^CG 586 CGCG NcoI 1316 CCATGG C^CATGG 2100 CCATGG PstI 1327 CTGCAG CTGCA^G 2134 CTGCAG SapI 357 GCTCTTC GCTCTTC(1/4) 429 GCTCTTC StuI 1427 AGGCCT AGG^CCT 1976 AGGCCT TaqI 203 TCGA T^CGA 1424 TCGA TthHB8I 203 TCGA T^CGA 1424 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbvI BcnI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TseI Tsp45I Tsp509I TspRI XcmI XmaCI XmaI