Restriction map for
pFN21AE5515
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AvaII
AviII BamHI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BseAI BsgI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I Bsu36I CelII Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRI EcoT22I FauI FbaI
FseI FspI HgaI Hin1I HindIII
HpaI KasI Ksp632I KspI MamI
MluI MroI MseI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 367 GGTACC G^GTACC
Asp718I 367 GGTACC G^GTACC
AspHI 217 GTGCTC G[AT]GC[AT]^C
AvaI 911 CCCGAG C^[CT]CG[GA]G
AvrII 429 CCTAGG C^CTAGG
BanI 367 GGTACC G^G[CT][GA]CC
BbvI 322 GCTGC GCAGC(8/12)
BglII 1055 AGATCT A^GATCT
BlnI 429 CCTAGG C^CTAGG
BsaI 609 GGTCTC GGTCTC(1/5)
BsaWI 50 ACCGGA [AT]^CCGG[AT]
BsiHKAI 217 GTGCTC G[AT]GC[AT]^C
BsmFI 964 GTCCC GGGAC(10/14)
BsmI 325 GCATTC GAATGC(1/-1)
BsoBI 911 CCCGAG C^[CT]CG[GA]G
BspHI 264 TCATGA T^CATGA
BsrDI 817 GCAATG GCAATG(2/0)
BstAPI 8 GCAGTAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 60 GGTCACC G^GT[ACGT]ACC
BstPI 60 GGTCACC G^GT[ACGT]ACC
BstYI 1055 AGATCT [GA]^GATC[CT]
Cac8I 155 GCTGGC GC[ACGT]^[ACGT]GC
DrdI 955 GACAAGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 60 GGTCACC G^GT[ACGT]ACC
EcoRV 1018 GATATC GAT^ATC
Fnu4HI 322 GCTGC GC^[ACGT]GC
HaeII 97 GGCGCT [GA]GCGC^[CT]
HincII 906 GTTGAC GT[CT]^[GA]AC
HindII 906 GTTGAC GT[CT]^[GA]AC
HinfI 137 GAATC G^A[ACGT]TC
ItaI 322 GCTGC GC^[ACGT]GC
KpnI 367 GGTACC GGTAC^C
MaeII 724 ACGT A^CGT
MfeI 356 CAATTG C^AATTG
MflI 1055 AGATCT [GA]^GATC[CT]
MunI 356 CAATTG C^AATTG
NspI 149 ACATGC [GA]CATG^[CT]
PshAI 694 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 264 TCATGA T^CATGA
SfcI 865 CTACAG C^T[GA][CT]AG
StuI 432 AGGCCT AGG^CCT
TfiI 137 GAATC G^A[AT]TC
TseI 322 GCTGC G^C[AT]GC
XhoII 1055 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 948 GAATTT [GA]^AATT[CT]
970 GAATTT
ApoI 948 GAATTT [GA]^AATT[CT]
970 GAATTT
BalI 72 TGGCCA TGG^CCA
572 TGGCCA
BcnI 482 CCCGG CC^[GC]GG
606 CCGGG
BmyI 217 GTGCTC G[AGT]GC[ACT]^C
828 GGGCAC
BseRI 29 CTCCTC GAGGAG(10/8)
980 CTCCTC
BsiYI 51 CCGGAGGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
599 CCAGTGTCCGG
BslI 51 CCGGAGGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
599 CCAGTGTCCGG
BsmAI 343 GAGAC GTCTC(1/5)
610 GTCTC
Bsp1286I 217 GTGCTC G[AGT]GC[ACT]^C
828 GGGCAC
BstNI 339 CCTGG CC^[AT]GG
1014 CCTGG
BstXI 672 CCACCTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1023 CCACATCAATGG
CfoI 98 GCGC GCG^C
153 GCGC
EcoRII 339 CCTGG ^CC[AT]GG
1014 CCTGG
EcoT14I 65 CCAAGG C^C[AT][AT]GG
429 CCTAGG
HhaI 98 GCGC GCG^C
153 GCGC
HinP1I 98 GCGC G^CGC
153 GCGC
MluNI 72 TGGCCA TGG^CCA
572 TGGCCA
MscI 72 TGGCCA TGG^CCA
572 TGGCCA
MspA1I 320 CAGCTG C[AC]G^C[GT]G
614 CAGCTG
MvaI 339 CCTGG CC^[AT]GG
1014 CCTGG
NciI 482 CCCGG CC^[GC]GG
606 CCGGG
NlaIV 367 GGTACC GG[ACGT]^[ACGT]CC
916 GGTTCC
PvuII 320 CAGCTG CAG^CTG
614 CAGCTG
StyI 65 CCAAGG C^C[AT][AT]GG
429 CCTAGG
TaqI 123 TCGA T^CGA
495 TCGA
Tsp45I 61 GTCAC ^GT[GC]AC
386 GTGAC
TthHB8I 123 TCGA T^CGA
495 TCGA
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AfaI AluI BfaI BpmI BsaJI
BsrI BssKI BstF5I DdeI DpnII
EaeI FokI HaeIII HapII HpaII
HphI MaeI MaeIII MboI MboII
MnlI MslI MspI MwoI NdeII
NlaIII RsaI Sau3AI ScrFI SfaNI
Tsp509I TspRI