Restriction map for
pFN21AE4458
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BseAI BsgI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI ClaI CpoI DraI DraII
DraIII DrdI Eco47III EcoNI EcoO109I
EcoO65I EcoRV FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NarI NciI
NdeI NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SbfI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AhdI 1028 GACATACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 769 GGATC GGATC(4/5)
AocI 688 CCTGAGG CC^T[ACGT]AGG
AseI 1095 ATTAAT AT^TAAT
AsnI 1095 ATTAAT AT^TAAT
Asp700I 1062 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1028 GACATACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 343 GGACC G^G[AT]CC
BbsI 543 GTCTTC GAAGAC(2/6)
BclI 1166 TGATCA T^GATCA
BmyI 1012 GGGCAC G[AGT]GC[ACT]^C
BpmI 303 CTGGAG CTGGAG(16/14)
BpuAI 543 GTCTTC GAAGAC(2/6)
BsaI 100 GAGACC GGTCTC(1/5)
BsaWI 156 ACCGGA [AT]^CCGG[AT]
BseRI 979 GAGGAG GAGGAG(10/8)
BsiEI 41 CGGCCG CG[GA][CT]^CG
Bsp1286I 1012 GGGCAC G[AGT]GC[ACT]^C
BspHI 488 TCATGA T^CATGA
BsrFI 39 GCCGGC [GA]^CCGG[CT]
BstYI 769 GGATCT [GA]^GATC[CT]
Bsu36I 688 CCTGAGG CC^T[ACGT]AGG
Cfr10I 39 GCCGGC [GA]^CCGG[CT]
DsaI 418 CCATGG C^C[GA][CT]GG
EaeI 41 CGGCCG [CT]^GGCC[GA]
EagI 41 CGGCCG C^GGCCG
Eam1105I 1028 GACATACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 41 CGGCCG C^GGCCG
Eco52I 41 CGGCCG C^GGCCG
Eco81I 688 CCTGAGG CC^T[ACGT]AGG
EcoRI 1062 GAATTC G^AATTC
EcoT14I 418 CCATGG C^C[AT][AT]GG
EcoT22I 1256 ATGCAT ATGCA^T
FauI 905 CCCGC CCCGC(4/6)
FbaI 1166 TGATCA T^GATCA
HgaI 22 GACGC GACGC(5/10)
MflI 769 GGATCT [GA]^GATC[CT]
MslI 787 CACAGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 115 CAGCGG C[AC]G^C[GT]G
MwoI 224 GCACTTTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 39 GCCGGC GCC^GGC
NcoI 418 CCATGG C^CATGG
NgoMIV 39 GCCGGC G^CCGGC
NsiI 1256 ATGCAT ATGCA^T
PflMI 1002 CCATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1095 ATTAAT AT^TAAT
RcaI 488 TCATGA T^CATGA
SapI 779 GCTCTTC GCTCTTC(1/4)
ScaI 857 AGTACT AGT^ACT
StyI 418 CCATGG C^C[AT][AT]GG
Van91I 1002 CCATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 882 CCAGGAGGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 769 GGATCT [GA]^GATC[CT]
XmnI 1062 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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BbvI 69 GCTGC GCAGC(8/12)
1120 GCTGC
BsaJI 368 CCCTGG C^C[ACGT][ACGT]GG
418 CCATGG
BsmFI 1026 GGGAC GGGAC(10/14)
366 GTCCC
BsrDI 791 GCAATG GCAATG(2/0)
908 GCAATG
Cac8I 39 GCCGGC GC[ACGT]^[ACGT]GC
334 GCTGGC
Cfr13I 62 GGGCC G^G[ACGT]CC
343 GGACC
EarI 780 CTCTTC CTCTTC(1/4)
853 GAAGAG
HapII 40 CCGG C^CGG
157 CCGG
HindIII 697 AAGCTT A^AGCTT
1400 AAGCTT
HpaII 40 CCGG C^CGG
157 CCGG
HphI 577 GGTGA GGTGA(8/7)
1189 TCACC
Ksp632I 780 CTCTTC CTCTTC(1/4)
853 GAAGAG
MspI 40 CCGG C^CGG
157 CCGG
NlaIV 61 GGGGCC GG[ACGT]^[ACGT]CC
900 GGAACC
Sau96I 62 GGGCC G^G[ACGT]CC
343 GGACC
TaqI 201 TCGA T^CGA
1104 TCGA
TseI 69 GCTGC G^C[AT]GC
1120 GCTGC
TthHB8I 201 TCGA T^CGA
1104 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI BfaI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HinfI ItaI MaeI MaeIII MboI
MboII MnlI MseI MvaI NdeII
NlaIII PleI RsaI Sau3AI ScrFI
SfaNI TfiI Tru9I Tsp45I Tsp509I
TspRI