Restriction map for
pFN21AE5214
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MaeI MamI
MfeI MluI MroI MspA1I MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tsp509I
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccII 292 CGCG CG^CG
AlwI 423 GATCC GGATC(4/5)
AspHI 573 GAGCAC G[AT]GC[AT]^C
AvaII 435 GGTCC G^G[AT]CC
BalI 411 TGGCCA TGG^CCA
BbeI 637 GGCGCC GGCGC^C
BbsI 588 GAAGAC GAAGAC(2/6)
BbvI 351 GCAGC GCAGC(8/12)
BcnI 627 CCCGG CC^[GC]GG
BglI 404 GCCACCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 570 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 570 GCTGAGC GC^T[ACGT]AGC
BpuAI 588 GAAGAC GAAGAC(2/6)
BsiHKAI 573 GAGCAC G[AT]GC[AT]^C
BsmAI 53 GTCTC GTCTC(1/5)
BsmFI 461 GGGAC GGGAC(10/14)
BspHI 386 TCATGA T^CATGA
BstAPI 215 GCAACAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 292 CGCG CG^CG
BstYI 422 AGATCC [GA]^GATC[CT]
CelII 570 GCTGAGC GC^T[ACGT]AGC
CfoI 638 GCGC GCG^C
Cfr13I 435 GGTCC G^G[ACGT]CC
DraII 434 AGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 445 GAAGAG CTCTTC(1/4)
EcoO109I 434 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 414 CCATGG C^C[AT][AT]GG
Fnu4HI 351 GCAGC GC^[ACGT]GC
HaeII 637 GGCGCC [GA]GCGC^[CT]
HgaI 133 GACGC GACGC(5/10)
HhaI 638 GCGC GCG^C
HinP1I 638 GCGC G^CGC
HinfI 463 GACTC G^A[ACGT]TC
ItaI 351 GCAGC GC^[ACGT]GC
KasI 637 GGCGCC G^GCGCC
Ksp632I 445 GAAGAG CTCTTC(1/4)
MaeIII 94 GTCAC ^GT[ACGT]AC
MflI 422 AGATCC [GA]^GATC[CT]
MluNI 411 TGGCCA TGG^CCA
MscI 411 TGGCCA TGG^CCA
MseI 263 TTAA T^TAA
MvnI 292 CGCG CG^CG
NarI 637 GGCGCC GG^CGCC
NciI 627 CCCGG CC^[GC]GG
NcoI 414 CCATGG C^CATGG
PleI 463 GACTC GAGTC(4/5)
PpuMI 434 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 430 CTGCAG CTGCA^G
RcaI 386 TCATGA T^CATGA
SapI 445 GAAGAGC GCTCTTC(1/4)
Sau96I 435 GGTCC G^G[ACGT]CC
SfcI 430 CTGCAG C^T[GA][CT]AG
SfoI 637 GGCGCC GGC^GCC
StyI 414 CCATGG C^C[AT][AT]GG
Tru9I 263 TTAA T^TAA
TseI 351 GCAGC G^C[AT]GC
Tsp45I 94 GTCAC ^GT[GC]AC
TspRI 170 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 405 CCACCGTGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 422 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 133 GACGCC G[GA]^CG[CT]C
637 GGCGCC
AfaI 100 GTAC GT^AC
321 GTAC
AluI 272 AGCT AG^CT
569 AGCT
AlwNI 98 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
565 CAGAAGCTG
BanII 46 GGGCTC G[GA]GC[CT]^C
448 GAGCCC
BpmI 241 CTCCAG CTGGAG(16/14)
274 CTGGAG
BsaHI 133 GACGCC G[GA]^CG[CT]C
637 GGCGCC
BsgI 147 CTGCAC GTGCAG(16/14)
349 GTGCAG
BspMI 102 ACCTGC ACCTGC(4/8)
432 GCAGGT
BsrBI 277 GAGCGG CCGCTC(-3/-3)
457 GAGCGG
BsrI 197 ACTGG ACTGG(1/-1)
243 CCAGT
BstF5I 360 GGATG GGATG(2/0)
630 GGATG
Cac8I 136 GCCCGC GC[ACGT]^[ACGT]GC
140 GCCTGC
DpnII 258 GATC ^GATC
423 GATC
EaeI 411 TGGCCA [CT]^GGCC[GA]
473 TGGCCG
FauI 137 CCCGC CCCGC(4/6)
459 GCGGG
FokI 360 GGATG GGATG(9/13)
630 GGATG
HaeIII 412 GGCC GG^CC
474 GGCC
HapII 355 CCGG C^CGG
628 CCGG
Hin1I 133 GACGCC G[GA]^CG[CT]C
637 GGCGCC
HpaII 355 CCGG C^CGG
628 CCGG
HphI 333 GGTGA GGTGA(8/7)
616 GGTGA
MaeII 116 ACGT A^CGT
347 ACGT
MboI 258 GATC ^GATC
423 GATC
MslI 4 CACAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
519 CATCTTGGTG
MspI 355 CCGG C^CGG
628 CCGG
NdeII 258 GATC ^GATC
423 GATC
RsaI 100 GTAC GT^AC
321 GTAC
Sau3AI 258 GATC ^GATC
423 GATC
SfaNI 10 GATGC GCATC(5/9)
336 GATGC
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
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AciI BanI BmyI BsaJI BseRI
BsiYI BslI Bsp1286I BssKI BstNI
DdeI DsaI EcoRII MboII MnlI
MvaI MwoI NlaIII NlaIV ScrFI