Restriction map for
pFN21AE6722
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AvaI
AviII AvrII BamHI BanI BbeI
BbvCI BcgI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsoBI Bsp1407I BspDI BspEI BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII CfoI ClaI CpoI DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccI 80 GTCGAC GT^[AC][GT]AC
AlwI 2259 GGATC GGATC(4/5)
AocI 652 CCTCAGG CC^T[ACGT]AGG
BanII 613 GGGCTC G[GA]GC[CT]^C
BbrPI 644 CACGTG CAC^GTG
BcnI 1580 CCCGG CC^[GC]GG
BsaAI 644 CACGTG [CT]AC^GT[GA]
BsgI 200 GTGCAG GTGCAG(16/14)
BsmBI 1473 GAGACG CGTCTC(1/5)
BspLU11I 1327 ACATGT A^CATGT
BsrFI 1899 GCCGGT [GA]^CCGG[CT]
BstAPI 600 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1568 CCATTCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 652 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1899 GCCGGT [GA]^CCGG[CT]
DraI 1192 TTTAAA TTT^AAA
Eco81I 652 CCTCAGG CC^T[ACGT]AGG
EcoNI 1414 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 16 GAATTC G^AATTC
EcoRV 1036 GATATC GAT^ATC
EcoT14I 751 CCTTGG C^C[AT][AT]GG
EcoT22I 1180 ATGCAT ATGCA^T
MaeII 645 ACGT A^CGT
MspA1I 1562 CAGCTG C[AC]G^C[GT]G
NciI 1580 CCCGG CC^[GC]GG
NheI 1584 GCTAGC G^CTAGC
NsiI 1180 ATGCAT ATGCA^T
PmaCI 644 CACGTG CAC^GTG
PmlI 644 CACGTG CAC^GTG
PpuMI 1287 AGGACCC [GA]G^G[AT]CC[CT]
PstI 598 CTGCAG CTGCA^G
PvuII 1562 CAGCTG CAG^CTG
SalI 80 GTCGAC G^TCGAC
StyI 751 CCTTGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 2043 CCGC CCGC(-3/-1)
2001 GCGG
AflIII 643 ACACGT A^C[GA][CT]GT
1327 ACATGT
AspHI 848 GTGCTC G[AT]GC[AT]^C
2216 GAGCAC
BalI 70 TGGCCA TGG^CCA
1023 TGGCCA
BbsI 918 GAAGAC GAAGAC(2/6)
562 GTCTTC
BciVI 1105 GGATAC GTATCC(6/5)
2011 GGATAC
BpuAI 918 GAAGAC GAAGAC(2/6)
562 GTCTTC
BsaJI 751 CCTTGG C^C[ACGT][ACGT]GG
2198 CCTGGG
BsiHKAI 848 GTGCTC G[AT]GC[AT]^C
2216 GAGCAC
BsmFI 1088 GTCCC GGGAC(10/14)
1355 GTCCC
BsmI 1458 GCATTC GAATGC(1/-1)
1922 GCATTC
BspHI 525 TCATGA T^CATGA
857 TCATGA
BsrDI 1494 CATTGC GCAATG(2/0)
1536 CATTGC
DraII 888 GGGGCCT [GA]G^G[ACGT]CC[CT]
1287 AGGACCC
EaeI 70 TGGCCA [CT]^GGCC[GA]
1023 TGGCCA
EcoO109I 888 GGGGCCT [GA]G^G[ACGT]CC[CT]
1287 AGGACCC
HapII 1581 CCGG C^CGG
1900 CCGG
HgaI 1475 GACGC GACGC(5/10)
1824 GCGTC
HincII 80 GTCGAC GT[CT]^[GA]AC
2164 GTCAAC
HindII 80 GTCGAC GT[CT]^[GA]AC
2164 GTCAAC
HpaII 1581 CCGG C^CGG
1900 CCGG
MluNI 70 TGGCCA TGG^CCA
1023 TGGCCA
MscI 70 TGGCCA TGG^CCA
1023 TGGCCA
MspI 1581 CCGG C^CGG
1900 CCGG
NspI 1178 ACATGC [GA]CATG^[CT]
1327 ACATGT
RcaI 525 TCATGA T^CATGA
857 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
AspI AvaII BbvI BfaI BglII
BmyI BpmI BsaI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV PflFI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XhoII
XmnI