Restriction map for
pFN21AB5261
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AvrII BalI BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII ClaI
DpnII DraI DraIII DrdI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI HincII HindII HindIII HpaI
Ksp632I MaeI MamI MboI MboII
MfeI MflI MluI MluNI MroI
MscI MseI MunI NcoI NdeI
NdeII NheI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
SacI SalI SapI Sau3AI SbfI
ScaI SexAI SfiI SfuI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Tru9I
Tsp509I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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ApaI 579 GGGCCC GGGCC^C
AspHI 148 GTGCTC G[AT]GC[AT]^C
AvaII 96 GGACC G^G[AT]CC
AviII 263 TGCGCA TGC^GCA
BanII 579 GGGCCC G[GA]GC[CT]^C
BglI 52 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 498 CTGCAC GTGCAG(16/14)
BsiHKAI 148 GTGCTC G[AT]GC[AT]^C
BsmFI 508 GTCCC GGGAC(10/14)
BsrI 114 ACTGG ACTGG(1/-1)
BssHII 196 GCGCGC G^CGCGC
BstF5I 486 CATCC GGATG(2/0)
BstXI 485 CCATCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 95 CGGACCG CG^G[AT]CCG
DdeI 151 CTCAG C^T[ACGT]AG
DraII 579 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 579 GGGCCCC [GA]G^G[ACGT]CC[CT]
FokI 486 CATCC GGATG(9/13)
FspI 263 TGCGCA TGC^GCA
HinfI 290 GAATC G^A[ACGT]TC
HphI 482 TCACC GGTGA(8/7)
MaeIII 481 GTCAC ^GT[ACGT]AC
MslI 486 CATCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII 411 CATG CATG^
NotI 78 GCGGCCGC GC^GGCCGC
NspI 410 ACATGC [GA]CATG^[CT]
PstI 172 CTGCAG CTGCA^G
RsrII 95 CGGACCG CG^G[AT]CCG
SgrAI 447 CGCCGGCG C[GA]^CCGG[CT]G
StuI 473 AGGCCT AGG^CCT
TaqI 551 TCGA T^CGA
TfiI 290 GAATC G^A[AT]TC
Tsp45I 481 GTCAC ^GT[GC]AC
TspRI 153 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 551 TCGA T^CGA
XcmI 566 CCAAGCTCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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Acc65I 311 GGTACC G^GTACC
320 GGTACC
AccI 406 GTCTAC GT^[AC][GT]AC
463 GTCTAC
Asp718I 311 GGTACC G^GTACC
320 GGTACC
AvaI 54 CCCGAG C^[CT]CG[GA]G
511 CCCGAG
BmyI 148 GTGCTC G[AGT]GC[ACT]^C
579 GGGCCC
BpmI 543 CTCCAG CTGGAG(16/14)
115 CTGGAG
BseRI 106 GAGGAG GAGGAG(10/8)
376 GAGGAG
BsoBI 54 CCCGAG C^[CT]CG[GA]G
511 CCCGAG
Bsp1286I 148 GTGCTC G[AGT]GC[ACT]^C
579 GGGCCC
KpnI 311 GGTACC GGTAC^C
320 GGTACC
KspI 44 CCGCGG CCGC^GG
82 CCGCGG
MaeII 383 ACGT A^CGT
479 ACGT
PvuII 121 CAGCTG CAG^CTG
175 CAGCTG
SacII 44 CCGCGG CCGC^GG
82 CCGCGG
SfaNI 536 GCATC GCATC(5/9)
548 GCATC
SfcI 172 CTGCAG C^T[GA][CT]AG
285 CTACAG
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
BanI BbeI BbvI BcnI BsaHI
BsaJI BsiEI BsiYI BslI BspMI
BsrFI BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DsaI EaeI
EagI EclXI Eco52I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII ItaI
KasI MnlI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NgoMIV NlaIV RsaI Sau96I ScrFI
SfoI TseI