Restriction map for
pFN21AE4588
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AvaI
AvaII AviII BalI BamHI BanI
BbeI BbrPI BciVI BglI BlpI
Bpu1102I BsaHI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsoBI BspDI BspEI
BspLU11I BspMI BsrBI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr13I
ClaI CpoI DraII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoT22I FauI
FseI FspI HgaI Hin1I HindIII
KasI KpnI KspI MluI MluNI
MroI MscI MslI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PvuI RsrII SacI
SacII SalI Sau96I SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI Tth111I Van91I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AclI 1647 AACGTT AA^CGTT
AflII 2656 CTTAAG C^TTAAG
Asp700I 673 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 2136 CCTAGG C^CTAGG
BanII 998 GGGCTC G[GA]GC[CT]^C
BcgI 2063 CGACATATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2656 CTTAAG C^TTAAG
BlnI 2136 CCTAGG C^CTAGG
BmyI 998 GGGCTC G[AGT]GC[ACT]^C
BsaAI 334 CACGTA [CT]AC^GT[GA]
BsaI 2232 GGTCTC GGTCTC(1/5)
BsmBI 546 CGTCTC CGTCTC(1/5)
Bsp1286I 998 GGGCTC G[AGT]GC[ACT]^C
BsrDI 886 CATTGC GCAATG(2/0)
BsrFI 1181 ACCGGC [GA]^CCGG[CT]
CfoI 1839 GCGC GCG^C
Cfr10I 1181 ACCGGC [GA]^CCGG[CT]
DraIII 2185 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1541 CCATGG C^C[GA][CT]GG
EaeI 2059 TGGCCG [CT]^GGCC[GA]
EcoRV 1591 GATATC GAT^ATC
HaeII 1838 AGCGCC [GA]GCGC^[CT]
HhaI 1839 GCGC GCG^C
HinP1I 1839 GCGC G^CGC
NcoI 1541 CCATGG C^CATGG
NdeI 2066 CATATG CA^TATG
NlaIV 1227 GGTTCC GG[ACGT]^[ACGT]CC
NspI 1118 GCATGC [GA]CATG^[CT]
Psp1406I 1647 AACGTT AA^CGTT
PstI 2344 CTGCAG CTGCA^G
SapI 1434 GCTCTTC GCTCTTC(1/4)
ScaI 688 AGTACT AGT^ACT
SphI 1118 GCATGC GCATG^C
SspI 2020 AATATT AAT^ATT
StuI 1811 AGGCCT AGG^CCT
XbaI 1260 TCTAGA T^CTAGA
XcmI 968 CCATTTGGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 673 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AciI 148 CCGC CCGC(-3/-1)
1910 GCGG
AlwI 19 GGATC GGATC(4/5)
1481 GGATC
AlwNI 89 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
317 CAGTGGCTG
BbsI 220 GAAGAC GAAGAC(2/6)
364 GAAGAC
BbvCI 86 CCTCAGC CCTCAGC(-5/-2)
403 GCTGAGG
BcnI 2014 CCGGG CC^[GC]GG
2685 CCGGG
BglII 115 AGATCT A^GATCT
785 AGATCT
BpuAI 220 GAAGAC GAAGAC(2/6)
364 GAAGAC
BsaBI 900 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2460 GATAAAGATC
BsgI 1024 GTGCAG GTGCAG(16/14)
1715 GTGCAG
BsiYI 965 CCTCCATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2043 CCTGACAATGG
BslI 965 CCTCCATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2043 CCTGACAATGG
BsmI 1906 GAATGC GAATGC(1/-1)
1046 GCATTC
Bsp1407I 767 TGTACA T^GTACA
2618 TGTACA
BspHI 902 TCATGA T^CATGA
1973 TCATGA
BsrGI 767 TGTACA T^GTACA
2618 TGTACA
BstBI 1103 TTCGAA TT^CGAA
2564 TTCGAA
BstYI 115 AGATCT [GA]^GATC[CT]
785 AGATCT
EcoRI 951 GAATTC G^AATTC
1406 GAATTC
EcoT14I 1541 CCATGG C^C[AT][AT]GG
2136 CCTAGG
HpaI 1249 GTTAAC GTT^AAC
2028 GTTAAC
MamI 900 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2460 GATAAAGATC
MflI 115 AGATCT [GA]^GATC[CT]
785 AGATCT
NciI 2014 CCGGG CC^[GC]GG
2685 CCGGG
NspV 1103 TTCGAA TT^CGAA
2564 TTCGAA
RcaI 902 TCATGA T^CATGA
1973 TCATGA
SfuI 1103 TTCGAA TT^CGAA
2564 TTCGAA
SmlI 1747 CTTGAG C^T[CT][GA]AG
2656 CTTAAG
SspBI 767 TGTACA T^GTACA
2618 TGTACA
StyI 1541 CCATGG C^C[AT][AT]GG
2136 CCTAGG
Tsp45I 6 GTCAC ^GT[GC]AC
2414 GTGAC
XhoII 115 AGATCT [GA]^GATC[CT]
785 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AcsI AfaI AluI ApoI AseI
AsnI BbvI BclI BfaI BpmI
BsaJI BseRI BsmAI BsmFI BsrI
BssKI BstF5I BstNI Cac8I DdeI
DpnII DraI EarI EcoRII FbaI
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MnlI MseI MspA1I
MspI MunI MvaI MwoI NdeII
NlaIII PshBI PvuII RsaI Sau3AI
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I