Restriction map for
pFN21AE5520
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI Asp700I Asp718I
AspEI AviII AvrII BalI BamHI
BbeI BbsI BbvCI BcgI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaHI BsaI BsaWI
BseAI BseRI BsiWI BsmI BspDI
BspEI BspMI BsrBI BsrDI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI Ksp632I
KspI MaeI MfeI MluI MluNI
MroI MscI MslI MunI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmeI PpuMI PshAI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 1172 GTCTAC GT^[AC][GT]AC
AccII 318 CGCG CG^CG
AciI 312 CCGC CCGC(-3/-1)
AlwNI 655 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1203 GGGCCC GGGCC^C
AseI 147 ATTAAT AT^TAAT
AsnI 147 ATTAAT AT^TAAT
AspHI 113 GTGCTC G[AT]GC[AT]^C
AspI 1166 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1295 CCCGAG C^[CT]CG[GA]G
AvaII 1165 GGACC G^G[AT]CC
BanII 1203 GGGCCC G[GA]GC[CT]^C
BbrPI 7 CACGTG CAC^GTG
BciVI 760 GGATAC GTATCC(6/5)
BcnI 1269 CCGGG CC^[GC]GG
BglII 103 AGATCT A^GATCT
BsaBI 1372 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 690 CTGCAC GTGCAG(16/14)
BsiEI 1266 CGACCG CG[GA][CT]^CG
BsiHKAI 113 GTGCTC G[AT]GC[AT]^C
BsiYI 1215 CCCTGTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1215 CCCTGTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 256 GAGACG CGTCTC(1/5)
BsmFI 1164 GGGAC GGGAC(10/14)
BsoBI 1295 CCCGAG C^[CT]CG[GA]G
BspHI 423 TCATGA T^CATGA
BspLU11I 550 ACATGT A^CATGT
BsrFI 846 ACCGGC [GA]^CCGG[CT]
BssSI 908 CACGAG CACGAG(-5/-1)
BstUI 318 CGCG CG^CG
Cac8I 1300 GCCTGC GC[ACGT]^[ACGT]GC
Cfr10I 846 ACCGGC [GA]^CCGG[CT]
DraI 193 TTTAAA TTT^AAA
EaeI 848 CGGCCA [CT]^GGCC[GA]
FauI 311 CCCGC CCCGC(4/6)
HindIII 570 AAGCTT A^AGCTT
HphI 124 TCACC GGTGA(8/7)
MamI 1372 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 1004 CAGCTG C[AC]G^C[GT]G
MvnI 318 CGCG CG^CG
NciI 1269 CCGGG CC^[GC]GG
NdeI 932 CATATG CA^TATG
PflFI 1166 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 7 CACGTG CAC^GTG
PmlI 7 CACGTG CAC^GTG
PshBI 147 ATTAAT AT^TAAT
PvuII 1004 CAGCTG CAG^CTG
RcaI 423 TCATGA T^CATGA
SfcI 434 CTACAG C^T[GA][CT]AG
SmlI 523 CTCAAG C^T[CT][GA]AG
SspI 1330 AATATT AAT^ATT
Tth111I 1166 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 851 CCAGGGTAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 550 ACATGT A^C[GA][CT]GT
721 ACGTGT
BanI 412 GGTGCC G^G[CT][GA]CC
618 GGCACC
BmyI 113 GTGCTC G[AGT]GC[ACT]^C
1203 GGGCCC
BsaAI 7 CACGTG [CT]AC^GT[GA]
720 TACGTG
Bsp1286I 113 GTGCTC G[AGT]GC[ACT]^C
1203 GGGCCC
Bsp1407I 543 TGTACA T^GTACA
779 TGTACA
BsrGI 543 TGTACA T^GTACA
779 TGTACA
BstYI 103 AGATCT [GA]^GATC[CT]
519 GGATCT
DsaI 263 CCATGG C^C[GA][CT]GG
536 CCATGG
EcoT14I 263 CCATGG C^C[AT][AT]GG
536 CCATGG
HapII 847 CCGG C^CGG
1269 CCGG
HpaII 847 CCGG C^CGG
1269 CCGG
MaeII 8 ACGT A^CGT
721 ACGT
MflI 103 AGATCT [GA]^GATC[CT]
519 GGATCT
MspI 847 CCGG C^CGG
1269 CCGG
NcoI 263 CCATGG C^CATGG
536 CCATGG
NspI 217 ACATGC [GA]CATG^[CT]
550 ACATGT
SfaNI 172 GATGC GCATC(5/9)
838 GATGC
SspBI 543 TGTACA T^GTACA
779 TGTACA
StyI 263 CCATGG C^C[AT][AT]GG
536 CCATGG
XhoII 103 AGATCT [GA]^GATC[CT]
519 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BpmI BsaJI BsmAI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HinfI ItaI MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I