Restriction map for
pFN21AE3671
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI BstXI CelII
CfoI Cfr10I ClaI CpoI DraIII
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII KasI KpnI
KspI MaeII MluI MluNI MroI
MscI MspA1I MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspI SwaI XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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Alw44I 557 GTGCAC G^TGCAC
AocI 2437 CCTAAGG CC^T[ACGT]AGG
ApaLI 557 GTGCAC G^TGCAC
BanI 2541 GGCACC G^G[CT][GA]CC
BbvI 2524 GCTGC GCAGC(8/12)
BsaI 621 GAGACC GGTCTC(1/5)
BspMI 7 ACCTGC ACCTGC(4/8)
BsrDI 190 CATTGC GCAATG(2/0)
Bst1107I 392 GTATAC GTA^TAC
BstZ17I 392 GTATAC GTA^TAC
Bsu36I 2437 CCTAAGG CC^T[ACGT]AGG
DrdI 2235 GACAGGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1735 CCATGG C^C[GA][CT]GG
Eco81I 2437 CCTAAGG CC^T[ACGT]AGG
EcoRI 2148 GAATTC G^AATTC
EcoRV 1914 GATATC GAT^ATC
Fnu4HI 2524 GCTGC GC^[ACGT]GC
HincII 2703 GTTAAC GT[CT]^[GA]AC
HindII 2703 GTTAAC GT[CT]^[GA]AC
HpaI 2703 GTTAAC GTT^AAC
ItaI 2524 GCTGC GC^[ACGT]GC
MwoI 877 GCAATTACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 1735 CCATGG C^CATGG
NspI 858 GCATGC [GA]CATG^[CT]
PflMI 1951 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 2425 AGTACT AGT^ACT
SexAI 2418 ACCTGGT A^CC[AT]GGT
SphI 858 GCATGC GCATG^C
StuI 2441 AGGCCT AGG^CCT
TseI 2524 GCTGC G^C[AT]GC
Tsp45I 1196 GTCAC ^GT[GC]AC
Van91I 1951 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 76 GTCTAC GT^[AC][GT]AC
392 GTATAC
AcsI 801 AAATTC [GA]^AATT[CT]
2148 GAATTC
AlwNI 2597 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
2857 CAGTGTCTG
ApoI 801 AAATTC [GA]^AATT[CT]
2148 GAATTC
AspHI 557 GTGCAC G[AT]GC[AT]^C
2384 GTGCTC
AspI 640 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2579 GACTTGGTC
BcnI 402 CCGGG CC^[GC]GG
1503 CCGGG
BglII 1141 AGATCT A^GATCT
2089 AGATCT
BmyI 557 GTGCAC G[AGT]GC[ACT]^C
2384 GTGCTC
BsaBI 1342 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2090 GATCTAAATC
BsgI 1640 CTGCAC GTGCAG(16/14)
2755 GTGCAG
BsiHKAI 557 GTGCAC G[AT]GC[AT]^C
2384 GTGCTC
BsmAI 621 GAGAC GTCTC(1/5)
1164 GTCTC
Bsp1286I 557 GTGCAC G[AGT]GC[ACT]^C
2384 GTGCTC
Bsp1407I 143 TGTACA T^GTACA
2076 TGTACA
BsrGI 143 TGTACA T^GTACA
2076 TGTACA
EcoT14I 713 CCAAGG C^C[AT][AT]GG
1735 CCATGG
HapII 402 CCGG C^CGG
1503 CCGG
HpaII 402 CCGG C^CGG
1503 CCGG
MamI 1342 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2090 GATCTAAATC
MfeI 196 CAATTG C^AATTG
845 CAATTG
MspI 402 CCGG C^CGG
1503 CCGG
MunI 196 CAATTG C^AATTG
845 CAATTG
NciI 402 CCGGG CC^[GC]GG
1503 CCGGG
PflFI 640 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2579 GACTTGGTC
SfcI 78 CTACAG C^T[GA][CT]AG
2594 CTACAG
SmlI 1166 CTCAAG C^T[CT][GA]AG
1886 CTCAAG
SpeI 2428 ACTAGT A^CTAGT
2707 ACTAGT
SspBI 143 TGTACA T^GTACA
2076 TGTACA
StyI 713 CCAAGG C^C[AT][AT]GG
1735 CCATGG
Tth111I 640 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2579 GACTTGGTC
XcmI 1817 CCAAAAAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2035 CCAGAAAAATACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AfaI AluI AlwI Asp700I AvaII
BbsI BfaI BpuAI BsaJI BseRI
BsiYI BslI BsmFI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRII EcoT22I FokI HaeIII
HinfI HphI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI NdeII NlaIII NlaIV
NsiI PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
Tru9I Tsp509I TspRI TthHB8I XhoII
XmnI