Restriction map for
pFN21AE6408
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII AccII AccIII AcyI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AviII AvrII BalI
BamHI BbeI BbrPI BcgI BciVI
BclI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
KasI KspI MamI MfeI MflI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI PvuI RcaI RsrII SacII
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI SwaI
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 935 GGTACC G^GTACC
AccI 911 GTAGAC GT^[AC][GT]AC
AclI 780 AACGTT AA^CGTT
AcsI 64 GAATTC [GA]^AATT[CT]
ApaI 953 GGGCCC GGGCC^C
ApoI 64 GAATTC [GA]^AATT[CT]
Asp718I 935 GGTACC G^GTACC
AspHI 682 GAGCTC G[AT]GC[AT]^C
AspI 304 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1187 CCCGAG C^[CT]CG[GA]G
BlpI 802 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 802 GCTGAGC GC^T[ACGT]AGC
BsiHKAI 682 GAGCTC G[AT]GC[AT]^C
BsmI 252 GAATGC GAATGC(1/-1)
BsoBI 1187 CCCGAG C^[CT]CG[GA]G
BssSI 1085 CACGAG CACGAG(-5/-1)
BstXI 185 CCAACAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 802 GCTGAGC GC^T[ACGT]AGC
CfoI 295 GCGC GCG^C
DraIII 244 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 204 GACTAGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 942 CCATGG C^C[GA][CT]GG
EcoNI 144 CCTTGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 64 GAATTC G^AATTC
EcoT14I 942 CCATGG C^C[AT][AT]GG
HhaI 295 GCGC GCG^C
HinP1I 295 GCGC G^CGC
HindIII 160 AAGCTT A^AGCTT
KpnI 935 GGTACC GGTAC^C
MslI 141 CATCCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 942 CCATGG C^CATGG
NheI 807 GCTAGC G^CTAGC
NspI 1494 ACATGC [GA]CATG^[CT]
PflFI 304 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1535 CCAATAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 302 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1110 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 780 AACGTT AA^CGTT
SacI 682 GAGCTC GAGCT^C
SbfI 312 CCTGCAGG CCTGCA^GG
SmlI 1569 CTTGAG C^T[CT][GA]AG
Sse8387I 312 CCTGCAGG CCTGCA^GG
StuI 835 AGGCCT AGG^CCT
StyI 942 CCATGG C^C[AT][AT]GG
TfiI 735 GAATC G^A[AT]TC
Tth111I 304 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1535 CCAATAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 717 CCACGAACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AvaII 303 GGACC G^G[AT]CC
435 GGACC
BanI 935 GGTACC G^G[CT][GA]CC
1387 GGTGCC
BanII 682 GAGCTC G[GA]GC[CT]^C
953 GGGCCC
BbsI 649 GTCTTC GAAGAC(2/6)
1396 GTCTTC
BbvCI 636 CCTCAGC CCTCAGC(-5/-2)
226 GCTGAGG
BcnI 593 CCGGG CC^[GC]GG
1137 CCGGG
BpuAI 649 GTCTTC GAAGAC(2/6)
1396 GTCTTC
BsaI 1572 GAGACC GGTCTC(1/5)
1530 GGTCTC
BseRI 598 GAGGAG GAGGAG(10/8)
985 GAGGAG
BsmFI 539 GGGAC GGGAC(10/14)
998 GGGAC
BsrBI 519 CCGCTC CCGCTC(-3/-3)
1467 GAGCGG
EarI 766 GAAGAG CTCTTC(1/4)
918 GAAGAG
HapII 593 CCGG C^CGG
1137 CCGG
HpaI 783 GTTAAC GTT^AAC
1221 GTTAAC
HpaII 593 CCGG C^CGG
1137 CCGG
Ksp632I 766 GAAGAG CTCTTC(1/4)
918 GAAGAG
MaeII 781 ACGT A^CGT
1351 ACGT
MspI 593 CCGG C^CGG
1137 CCGG
NciI 593 CCGGG CC^[GC]GG
1137 CCGGG
PleI 319 GACTC GAGTC(4/5)
1115 GAGTC
TaqI 1049 TCGA T^CGA
1292 TCGA
Tsp45I 618 GTCAC ^GT[GC]AC
1478 GTGAC
TspRI 372 CACTG CA[GC]TG[ACGT][ACGT]^
1122 CACTG
TthHB8I 1049 TCGA T^CGA
1292 TCGA
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BbvI BfaI BmyI BpmI BsaJI
BsgI BsiYI BslI BsmAI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI Tru9I TseI Tsp509I