Restriction map for
pFN21AE6714
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# NO CUT ENZYMES ( 89 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AviII BbeI
BbrPI BcgI BciVI BfrI BglII
BsaAI BsaBI BseAI BsmBI BspEI
BspLU11I BssHII BssSI Bst1107I BstBI
BstZ17I CpoI DraI DrdI Eam1105I
Eco47III EcoRV EcoT22I FseI FspI
HindIII HpaI KasI KpnI KspI
MamI MluI MroI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 3270 CGCG CG^CG
AocI 713 CCTCAGG CC^T[ACGT]AGG
ApaI 2671 GGGCCC GGGCC^C
AspI 2389 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2368 CCTAGG C^CTAGG
BlnI 2368 CCTAGG C^CTAGG
BlpI 482 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 482 GCTCAGC GC^T[ACGT]AGC
BsiEI 3276 CGGCCG CG[GA][CT]^CG
BsiWI 402 CGTACG C^GTACG
BspDI 2759 ATCGAT AT^CGAT
BspHI 3317 TCATGA T^CATGA
BsrBI 214 GAGCGG CCGCTC(-3/-3)
BsrFI 3278 GCCGGT [GA]^CCGG[CT]
BstAPI 2246 GCAGGCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2001 GGTCACC G^GT[ACGT]ACC
BstPI 2001 GGTCACC G^GT[ACGT]ACC
BstUI 3270 CGCG CG^CG
Bsu36I 713 CCTCAGG CC^T[ACGT]AGG
CelII 482 GCTCAGC GC^T[ACGT]AGC
Cfr10I 3278 GCCGGT [GA]^CCGG[CT]
ClaI 2759 ATCGAT AT^CGAT
DraIII 2717 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3276 CGGCCG C^GGCCG
EclXI 3276 CGGCCG C^GGCCG
Eco52I 3276 CGGCCG C^GGCCG
Eco81I 713 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2001 GGTCACC G^GT[ACGT]ACC
EcoRI 2848 GAATTC G^AATTC
HaeII 3488 AGCGCC [GA]GCGC^[CT]
MfeI 2537 CAATTG C^AATTG
MunI 2537 CAATTG C^AATTG
MvnI 3270 CGCG CG^CG
PaeR7I 3453 CTCGAG C^TCGAG
PflFI 2389 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3245 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 3317 TCATGA T^CATGA
SapI 3321 GAAGAGC GCTCTTC(1/4)
ScaI 1118 AGTACT AGT^ACT
SexAI 3134 ACCTGGT A^CC[AT]GGT
SmlI 3453 CTCGAG C^T[CT][GA]AG
SspI 1891 AATATT AAT^ATT
StuI 14 AGGCCT AGG^CCT
Tth111I 2389 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3453 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AvaI 2978 CTCGGG C^[CT]CG[GA]G
3453 CTCGAG
BclI 3045 TGATCA T^GATCA
3545 TGATCA
BsaI 1747 GAGACC GGTCTC(1/5)
464 GGTCTC
BsaWI 2276 ACCGGA [AT]^CCGG[AT]
2735 TCCGGT
BsmI 636 GAATGC GAATGC(1/-1)
1257 GAATGC
BsoBI 2978 CTCGGG C^[CT]CG[GA]G
3453 CTCGAG
Bsp1407I 598 TGTACA T^GTACA
1529 TGTACA
BspMI 300 ACCTGC ACCTGC(4/8)
616 ACCTGC
BsrGI 598 TGTACA T^GTACA
1529 TGTACA
FbaI 3045 TGATCA T^GATCA
3545 TGATCA
MaeII 1283 ACGT A^CGT
1966 ACGT
PflMI 11 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
287 CCACTGACTGG
PshAI 501 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2052 GACCCAGGTC
SspBI 598 TGTACA T^GTACA
1529 TGTACA
Van91I 11 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
287 CCACTGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII