Restriction map for
pFN21AB7141
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspDI BspEI
BspHI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstUI BstXI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT14I FauI FbaI FseI
FspI HaeII HaeIII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MspA1I MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I StuI StyI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AciI 630 GCGG CCGC(-3/-1)
AluI 624 AGCT AG^CT
AocI 372 CCTAAGG CC^T[ACGT]AGG
AvaII 1002 GGTCC G^G[AT]CC
BbsI 775 GAAGAC GAAGAC(2/6)
BfaI 145 CTAG C^TAG
BpuAI 775 GAAGAC GAAGAC(2/6)
BsaJI 644 CCACGG C^C[ACGT][ACGT]GG
BsaWI 608 ACCGGA [AT]^CCGG[AT]
BsgI 921 CTGCAC GTGCAG(16/14)
BsiYI 326 CCGTTGATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 326 CCGTTGATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspLU11I 10 ACATGT A^CATGT
BspMI 316 ACCTGC ACCTGC(4/8)
BsrDI 321 CATTGC GCAATG(2/0)
BssKI 33 CCTGG ^CC[ACGT]GG
BstBI 772 TTCGAA TT^CGAA
BstEII 588 GGTCACC G^GT[ACGT]ACC
BstF5I 594 CATCC GGATG(2/0)
BstNI 33 CCTGG CC^[AT]GG
BstPI 588 GGTCACC G^GT[ACGT]ACC
BstYI 100 GGATCT [GA]^GATC[CT]
Bsu36I 372 CCTAAGG CC^T[ACGT]AGG
Cfr13I 1002 GGTCC G^G[ACGT]CC
DdeI 373 CTAAG C^T[ACGT]AG
DraI 840 TTTAAA TTT^AAA
DraII 1001 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 644 CCACGG C^C[GA][CT]GG
Eco81I 372 CCTAAGG CC^T[ACGT]AGG
EcoO109I 1001 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 588 GGTCACC G^GT[ACGT]ACC
EcoRII 33 CCTGG ^CC[AT]GG
EcoT22I 188 ATGCAT ATGCA^T
FokI 594 CATCC GGATG(9/13)
HapII 609 CCGG C^CGG
HpaII 609 CCGG C^CGG
MaeI 145 CTAG C^TAG
MaeII 562 ACGT A^CGT
MflI 100 GGATCT [GA]^GATC[CT]
MspI 609 CCGG C^CGG
MvaI 33 CCTGG CC^[AT]GG
NsiI 188 ATGCAT ATGCA^T
NspV 772 TTCGAA TT^CGAA
PpuMI 1001 AGGTCCT [GA]G^G[AT]CC[CT]
Sau96I 1002 GGTCC G^G[ACGT]CC
ScrFI 33 CCTGG CC^[ACGT]GG
SfuI 772 TTCGAA TT^CGAA
SmlI 1006 CTTGAG C^T[CT][GA]AG
SphI 759 GCATGC GCATG^C
TaqI 773 TCGA T^CGA
TspRI 924 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 773 TCGA T^CGA
XhoII 100 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 10 ACATGT A^C[GA][CT]GT
562 ACGTGT
BbvI 299 GCAGC GCAGC(8/12)
746 GCTGC
BsmI 83 GCATTC GAATGC(1/-1)
307 GCATTC
Bsp1407I 281 TGTACA T^GTACA
565 TGTACA
BsrGI 281 TGTACA T^GTACA
565 TGTACA
BsrI 236 ACTGG ACTGG(1/-1)
925 ACTGG
Cac8I 123 GCAAGC GC[ACGT]^[ACGT]GC
759 GCATGC
EarI 157 CTCTTC CTCTTC(1/4)
663 GAAGAG
HphI 314 TCACC GGTGA(8/7)
590 TCACC
Ksp632I 157 CTCTTC CTCTTC(1/4)
663 GAAGAG
MslI 63 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
279 CATGTACATG
NspI 10 ACATGT [GA]CATG^[CT]
759 GCATGC
SfaNI 481 GATGC GCATC(5/9)
689 GCATC
SspBI 281 TGTACA T^GTACA
565 TGTACA
SspI 94 AATATT AAT^ATT
473 AATATT
TseI 299 GCAGC G^C[AT]GC
746 GCTGC
Tsp45I 168 GTCAC ^GT[GC]AC
589 GTCAC
XcmI 410 CCATCTGGATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
780 CCACAGCAATTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AccI AcsI AfaI AlwI ApoI
DpnII Fnu4HI HinfI ItaI MaeIII
MboI MboII MnlI MseI NdeII
NlaIII RsaI Sau3AI TfiI Tru9I
Tsp509I