Restriction map for
pFN21AE1320
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspI AviII AvrII BbeI
BbrPI BbsI BbvCI BcgI BclI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
Tth111I Van91I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccII 927 CGCG CG^CG
AfaI 870 GTAC GT^AC
AhdI 601 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 528 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 601 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 259 GGATCC G^GATCC
BciVI 273 GGATAC GTATCC(6/5)
BglI 26 GCCTGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 324 AGATCT A^GATCT
BpmI 1252 CTCCAG CTGGAG(16/14)
BsmAI 428 GAGAC GTCTC(1/5)
BsrDI 827 GCAATG GCAATG(2/0)
BsrI 971 ACTGG ACTGG(1/-1)
BssSI 1240 CTCGTG CACGAG(-5/-1)
BstUI 927 CGCG CG^CG
DsaI 1226 CCATGG C^C[GA][CT]GG
Eam1105I 601 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1191 CCTAGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1226 CCATGG C^C[AT][AT]GG
HgaI 925 GACGC GACGC(5/10)
HphI 471 GGTGA GGTGA(8/7)
MvnI 927 CGCG CG^CG
NcoI 1226 CCATGG C^CATGG
NdeI 239 CATATG CA^TATG
NspI 35 GCATGC [GA]CATG^[CT]
PvuII 502 CAGCTG CAG^CTG
RsaI 870 GTAC GT^AC
SfaNI 1214 GCATC GCATC(5/9)
SfcI 726 CTACAG C^T[GA][CT]AG
SphI 35 GCATGC GCATG^C
SspI 251 AATATT AAT^ATT
StyI 1226 CCATGG C^C[AT][AT]GG
Tsp45I 230 GTGAC ^GT[GC]AC
XmnI 528 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaII 608 GGTCC G^G[AT]CC
1160 GGTCC
BalI 498 TGGCCA TGG^CCA
939 TGGCCA
BanI 541 GGCACC G^G[CT][GA]CC
558 GGCACC
BcnI 12 CCCGG CC^[GC]GG
31 CCCGG
BseRI 1106 CTCCTC GAGGAG(10/8)
1198 GAGGAG
BsmFI 968 GGGAC GGGAC(10/14)
609 GTCCC
HapII 13 CCGG C^CGG
32 CCGG
HpaII 13 CCGG C^CGG
32 CCGG
MaeII 446 ACGT A^CGT
712 ACGT
MaeIII 230 GTGAC ^GT[ACGT]AC
244 GTAAC
MluNI 498 TGGCCA TGG^CCA
939 TGGCCA
MscI 498 TGGCCA TGG^CCA
939 TGGCCA
MseI 373 TTAA T^TAA
434 TTAA
MslI 531 CACCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1224 CACCATGGTG
MspA1I 502 CAGCTG C[AC]G^C[GT]G
1246 CAGCGG
MspI 13 CCGG C^CGG
32 CCGG
NciI 12 CCCGG CC^[GC]GG
31 CCCGG
PaeR7I 906 CTCGAG C^TCGAG
1009 CTCGAG
StuI 755 AGGCCT AGG^CCT
1072 AGGCCT
Tru9I 373 TTAA T^TAA
434 TTAA
XhoI 906 CTCGAG C^TCGAG
1009 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaI
BanII BbvI BfaI BmyI BsaJI
BsgI BsiHKAI BsiYI BslI BsoBI
Bsp1286I BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EaeI
EarI EcoRII Fnu4HI FokI HaeIII
HinfI ItaI Ksp632I MaeI MboI
MboII MflI MnlI MvaI MwoI
NdeII NlaIII NlaIV PleI SacI
SapI Sau3AI Sau96I ScrFI SmlI
TaqI TfiI TseI Tsp509I TspRI
TthHB8I XhoII