Restriction map for
pFN21AE1359
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AviII AvrII BanI BbeI
BbrPI BbsI BbvCI BcgI BclI
BfaI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI BstBI BstEII BstPI
BstXI Bsu36I CelII ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HindIII HinfI HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MroI MslI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PleI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 63 CGCG CG^CG
AcyI 1051 GGCGTC G[GA]^CG[CT]C
AfaI 319 GTAC GT^AC
AgeI 521 ACCGGT A^CCGGT
AseI 690 ATTAAT AT^TAAT
AsnI 690 ATTAAT AT^TAAT
Asp700I 1038 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1026 GAGCTC G[AT]GC[AT]^C
AvaI 1118 CCCGAG C^[CT]CG[GA]G
AvaII 162 GGACC G^G[AT]CC
BalI 137 TGGCCA TGG^CCA
BamHI 120 GGATCC G^GATCC
BciVI 807 GTATCC GTATCC(6/5)
BcnI 252 CCGGG CC^[GC]GG
BpmI 81 CTCCAG CTGGAG(16/14)
BsaHI 1051 GGCGTC G[GA]^CG[CT]C
BsaI 490 GAGACC GGTCTC(1/5)
BsaWI 521 ACCGGT [AT]^CCGG[AT]
BsgI 203 GTGCAG GTGCAG(16/14)
BsiHKAI 1026 GAGCTC G[AT]GC[AT]^C
BsmBI 1018 GAGACG CGTCTC(1/5)
BsoBI 1118 CCCGAG C^[CT]CG[GA]G
BspMI 762 GCAGGT ACCTGC(4/8)
BsrFI 521 ACCGGT [GA]^CCGG[CT]
BstAPI 196 GCAGTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 63 CGCG CG^CG
Cfr10I 521 ACCGGT [GA]^CCGG[CT]
DdeI 322 CTGAG C^T[ACGT]AG
DraII 704 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 732 GACTATTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 137 TGGCCA [CT]^GGCC[GA]
EcoO109I 704 AGGCCCT [GA]G^G[ACGT]CC[CT]
HgaI 1052 GCGTC GACGC(5/10)
Hin1I 1051 GGCGTC G[GA]^CG[CT]C
HincII 865 GTTGAC GT[CT]^[GA]AC
HindII 865 GTTGAC GT[CT]^[GA]AC
MluNI 137 TGGCCA TGG^CCA
MscI 137 TGGCCA TGG^CCA
MvnI 63 CGCG CG^CG
NciI 252 CCGGG CC^[GC]GG
NspI 758 ACATGC [GA]CATG^[CT]
PinAI 521 ACCGGT A^CCGGT
PshBI 690 ATTAAT AT^TAAT
PstI 418 CTGCAG CTGCA^G
RsaI 319 GTAC GT^AC
SacI 1026 GAGCTC GAGCT^C
SfcI 418 CTGCAG C^T[GA][CT]AG
TaqI 146 TCGA T^CGA
TthHB8I 146 TCGA T^CGA
XmnI 1038 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 754 GTATAC GT^[AC][GT]AC
890 GTATAC
AcsI 674 AAATTC [GA]^AATT[CT]
877 GAATTT
AflII 381 CTTAAG C^TTAAG
709 CTTAAG
ApoI 674 AAATTC [GA]^AATT[CT]
877 GAATTT
BanII 1026 GAGCTC G[GA]GC[CT]^C
1174 GGGCTC
BfrI 381 CTTAAG C^TTAAG
709 CTTAAG
BmyI 1026 GAGCTC G[AGT]GC[ACT]^C
1174 GGGCTC
BseRI 1160 CTCCTC GAGGAG(10/8)
1177 CTCCTC
Bsp1286I 1026 GAGCTC G[AGT]GC[ACT]^C
1174 GGGCTC
Bst1107I 754 GTATAC GTA^TAC
890 GTATAC
BstYI 120 GGATCC [GA]^GATC[CT]
846 GGATCT
BstZ17I 754 GTATAC GTA^TAC
890 GTATAC
CfoI 12 GCGC GCG^C
62 GCGC
DsaI 683 CCATGG C^C[GA][CT]GG
1004 CCATGG
EarI 413 CTCTTC CTCTTC(1/4)
87 GAAGAG
HhaI 12 GCGC GCG^C
62 GCGC
HinP1I 12 GCGC G^CGC
62 GCGC
HphI 680 TCACC GGTGA(8/7)
1181 TCACC
Ksp632I 413 CTCTTC CTCTTC(1/4)
87 GAAGAG
MaeII 101 ACGT A^CGT
271 ACGT
MaeIII 103 GTGAC ^GT[ACGT]AC
646 GTGAC
MflI 120 GGATCC [GA]^GATC[CT]
846 GGATCT
MspA1I 310 CCGCTG C[AC]G^C[GT]G
1212 CAGCGG
NcoI 683 CCATGG C^CATGG
1004 CCATGG
Tsp45I 103 GTGAC ^GT[GC]AC
646 GTGAC
XhoII 120 GGATCC [GA]^GATC[CT]
846 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BsiYI BslI BsmAI BsmFI BsrBI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DpnII EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HpaII
ItaI MboI MboII MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
SmlI StyI Tru9I TseI Tsp509I
TspRI