################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvaII AviII AvrII BalI BamHI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI KpnI Ksp632I MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsaI RsrII SacI SalI SapI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I TspRI Tth111I Van91I XbaI XhoII XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AhdI 274 GACGAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 19 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 308 GGGCCC GGGCC^C AspEI 274 GACGAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 298 GAGCAC G[AT]GC[AT]^C BanI 319 GGCGCC G^G[CT][GA]CC BbeI 319 GGCGCC GGCGC^C BcgI 113 GCATGGGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 211 GCCTGCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 415 GCTGAGC GC^T[ACGT]AGC Bpu1102I 415 GCTGAGC GC^T[ACGT]AGC BsgI 58 CTGCAC GTGCAG(16/14) BsiHKAI 298 GAGCAC G[AT]GC[AT]^C BspHI 197 TCATGA T^CATGA BstEII 150 GGTGACC G^GT[ACGT]ACC BstPI 150 GGTGACC G^GT[ACGT]ACC BstXI 77 CCAGCGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 415 GCTGAGC GC^T[ACGT]AGC DpnII 201 GATC ^GATC DraII 308 GGGCCCC [GA]G^G[ACGT]CC[CT] DsaI 347 CCGCGG C^C[GA][CT]GG EaeI 218 TGGCCG [CT]^GGCC[GA] Eam1105I 274 GACGAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 402 CCTCGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 308 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoO65I 150 GGTGACC G^GT[ACGT]ACC HphI 150 GGTGA GGTGA(8/7) KasI 319 GGCGCC G^GCGCC KspI 347 CCGCGG CCGC^GG MaeIII 151 GTGAC ^GT[ACGT]AC MboI 201 GATC ^GATC MboII 203 TCTTC GAAGA(8/7) MslI 108 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 319 GGCGCC GG^CGCC NdeII 201 GATC ^GATC PaeR7I 52 CTCGAG C^TCGAG PstI 6 CTGCAG CTGCA^G RcaI 197 TCATGA T^CATGA SacII 347 CCGCGG CCGC^GG Sau3AI 201 GATC ^GATC SfcI 6 CTGCAG C^T[GA][CT]AG SfoI 319 GGCGCC GGC^GCC SmaI 312 CCCGGG CCC^GGG SmlI 52 CTCGAG C^T[CT][GA]AG StuI 225 AGGCCT AGG^CCT Tsp45I 151 GTGAC ^GT[GC]AC XcmI 104 CCAGCATGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 52 CTCGAG C^TCGAG XmaCI 312 CCCGGG C^CCGGG XmaI 312 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccII 145 CGCG CG^CG 348 CGCG AvaI 52 CTCGAG C^[CT]CG[GA]G 312 CCCGGG BanII 308 GGGCCC G[GA]GC[CT]^C 351 GGGCTC BsmFI 101 GTCCC GGGAC(10/14) 282 GTCCC BsoBI 52 CTCGAG C^[CT]CG[GA]G 312 CCCGGG BsrBI 246 CCGCTC CCGCTC(-3/-3) 418 GAGCGG BstF5I 186 CATCC GGATG(2/0) 231 CATCC BstUI 145 CGCG CG^CG 348 CGCG DdeI 37 CTGAG C^T[ACGT]AG 416 CTGAG EcoT14I 161 CCAAGG C^C[AT][AT]GG 269 CCAAGG FokI 186 CATCC GGATG(9/13) 231 CATCC HgaI 172 GACGC GACGC(5/10) 334 GACGC MvnI 145 CGCG CG^CG 348 CGCG StyI 161 CCAAGG C^C[AT][AT]GG 269 CCAAGG TaqI 53 TCGA T^CGA 263 TCGA TthHB8I 53 TCGA T^CGA 263 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AcyI AluI BbvI BcnI BmyI BsaHI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI TseI