Restriction map for
pFN21AE1570
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI Aor51HI ApoI AscI Asp700I
Asp718I AspI AvrII BamHI BbrPI
BcgI BciVI BclI BfrI BlnI
BsaAI BsiEI BsiWI Bsp1407I BspDI
BspHI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstPI Cfr10I ClaI
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI MfeI MluI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI TfiI Tsp509I Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 1580 GTATAC GT^[AC][GT]AC
AcyI 213 GGCGCC G[GA]^CG[CT]C
AflIII 1798 ACATGT A^C[GA][CT]GT
AhdI 58 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 205 GTGCAC G^TGCAC
ApaI 238 GGGCCC GGGCC^C
ApaLI 205 GTGCAC G^TGCAC
AseI 907 ATTAAT AT^TAAT
AsnI 907 ATTAAT AT^TAAT
AspEI 58 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1079 TGCGCA TGC^GCA
BalI 1064 TGGCCA TGG^CCA
BbeI 213 GGCGCC GGCGC^C
BbvCI 145 GCTGAGG CCTCAGC(-5/-2)
BsaBI 582 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 213 GGCGCC G[GA]^CG[CT]C
BsmBI 2019 CGTCTC CGTCTC(1/5)
BsmI 540 GAATGC GAATGC(1/-1)
BspLU11I 1798 ACATGT A^CATGT
BsrBI 1957 GAGCGG CCGCTC(-3/-3)
BsrDI 1082 GCAATG GCAATG(2/0)
Bst1107I 1580 GTATAC GTA^TAC
BstAPI 1116 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1742 CCAAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1580 GTATAC GTA^TAC
DraIII 391 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 58 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1560 CCTAAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1375 GATATC GAT^ATC
FspI 1079 TGCGCA TGC^GCA
Hin1I 213 GGCGCC G[GA]^CG[CT]C
KasI 213 GGCGCC G^GCGCC
KspI 289 CCGCGG CCGC^GG
MaeII 179 ACGT A^CGT
MamI 582 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1064 TGGCCA TGG^CCA
MscI 1064 TGGCCA TGG^CCA
MseI 908 TTAA T^TAA
MslI 1626 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 213 GGCGCC GG^CGCC
PaeR7I 1941 CTCGAG C^TCGAG
PshAI 897 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 907 ATTAAT AT^TAAT
PstI 352 CTGCAG CTGCA^G
SacII 289 CCGCGG CCGC^GG
SfoI 213 GGCGCC GGC^GCC
SmlI 1941 CTCGAG C^T[CT][GA]AG
Tru9I 908 TTAA T^TAA
XcmI 720 CCAGTTCCTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1941 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 202 CGCG CG^CG
290 CGCG
AfaI 362 GTAC GT^AC
444 GTAC
AocI 1141 CCTGAGG CC^T[ACGT]AGG
1369 CCTGAGG
BanI 213 GGCGCC G^G[CT][GA]CC
1861 GGCACC
BfaI 844 CTAG C^TAG
1993 CTAG
BglI 37 GCCTTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
796 GCCATGGTGGC
BglII 1541 AGATCT A^GATCT
1937 AGATCT
BstUI 202 CGCG CG^CG
290 CGCG
Bsu36I 1141 CCTGAGG CC^T[ACGT]AGG
1369 CCTGAGG
EarI 307 CTCTTC CTCTTC(1/4)
1192 CTCTTC
Eco81I 1141 CCTGAGG CC^T[ACGT]AGG
1369 CCTGAGG
Ksp632I 307 CTCTTC CTCTTC(1/4)
1192 CTCTTC
MaeI 844 CTAG C^TAG
1993 CTAG
MvnI 202 CGCG CG^CG
290 CGCG
NcoI 797 CCATGG C^CATGG
1628 CCATGG
PflMI 321 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1067 CCAAGACCTGG
RsaI 362 GTAC GT^AC
444 GTAC
SfcI 352 CTGCAG C^T[GA][CT]AG
556 CTACAG
SphI 1656 GCATGC GCATG^C
1718 GCATGC
StuI 1526 AGGCCT AGG^CCT
1746 AGGCCT
TaqI 1942 TCGA T^CGA
2009 TCGA
TthHB8I 1942 TCGA T^CGA
2009 TCGA
Van91I 321 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1067 CCAAGACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccIII AciI AluI AlwI AlwNI
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MnlI MroI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
SacI Sau3AI Sau96I ScrFI SfaNI
SmaI StyI TseI Tsp45I TspRI
XhoII XmaCI XmaI