Restriction map for
pFN21AE1426
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BbrPI BbsI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BspDI
BsrDI BsrFI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I FbaI FseI FspI HincII
HindII HindIII HpaI HphI KpnI
KspI MamI MfeI MluI MluNI
MscI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
StuI StyI SwaI Tsp45I Tth111I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 475 GTCTAC GT^[AC][GT]AC
AccIII 100 TCCGGA T^CCGGA
AcyI 72 GGCGCC G[GA]^CG[CT]C
AflIII 740 ACATGT A^C[GA][CT]GT
AvaI 57 CCCGGG C^[CT]CG[GA]G
BanI 72 GGCGCC G^G[CT][GA]CC
BanII 124 GAGCCC G[GA]GC[CT]^C
BbeI 72 GGCGCC GGCGC^C
BbvCI 540 CCTCAGC CCTCAGC(-5/-2)
BfaI 928 CTAG C^TAG
BmyI 124 GAGCCC G[AGT]GC[ACT]^C
BsaHI 72 GGCGCC G[GA]^CG[CT]C
BsaI 865 GAGACC GGTCTC(1/5)
BsaWI 100 TCCGGA [AT]^CCGG[AT]
BseAI 100 TCCGGA T^CCGGA
BseRI 169 GAGGAG GAGGAG(10/8)
BsmFI 1225 GTCCC GGGAC(10/14)
BsoBI 57 CCCGGG C^[CT]CG[GA]G
Bsp1286I 124 GAGCCC G[AGT]GC[ACT]^C
BspEI 100 TCCGGA T^CCGGA
BspHI 206 TCATGA T^CATGA
BspLU11I 740 ACATGT A^CATGT
BsrBI 887 GAGCGG CCGCTC(-3/-3)
BssSI 531 CACGAG CACGAG(-5/-1)
BstXI 223 CCAGAATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 454 TTTAAA TTT^AAA
EaeI 1156 CGGCCA [CT]^GGCC[GA]
EcoNI 768 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 72 GGCGCC [GA]GCGC^[CT]
Hin1I 72 GGCGCC G[GA]^CG[CT]C
KasI 72 GGCGCC G^GCGCC
MaeI 928 CTAG C^TAG
MroI 100 TCCGGA T^CCGGA
NarI 72 GGCGCC GG^CGCC
PpuMI 60 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 206 TCATGA T^CATGA
SfoI 72 GGCGCC GGC^GCC
SmaI 57 CCCGGG CCC^GGG
SphI 392 GCATGC GCATG^C
SspI 227 AATATT AAT^ATT
TfiI 824 GATTC G^A[AT]TC
XcmI 828 CCATGACATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 57 CCCGGG C^CCGGG
XmaI 57 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AcsI 322 AAATTT [GA]^AATT[CT]
997 GAATTT
ApoI 322 AAATTT [GA]^AATT[CT]
997 GAATTT
BcnI 57 CCCGG CC^[GC]GG
58 CCGGG
BglII 162 AGATCT A^GATCT
350 AGATCT
BpmI 166 CTGGAG CTGGAG(16/14)
803 CTGGAG
BsmAI 313 GAGAC GTCTC(1/5)
865 GAGAC
Bsp1407I 239 TGTACA T^GTACA
1084 TGTACA
BspMI 724 ACCTGC ACCTGC(4/8)
1036 ACCTGC
BsrGI 239 TGTACA T^GTACA
1084 TGTACA
BstYI 162 AGATCT [GA]^GATC[CT]
350 AGATCT
CfoI 49 GCGC GCG^C
73 GCGC
DpnII 163 GATC ^GATC
351 GATC
DraII 60 GGGTCCT [GA]G^G[ACGT]CC[CT]
582 AGGCCCC
DsaI 143 CCACGG C^C[GA][CT]GG
397 CCACGG
EcoO109I 60 GGGTCCT [GA]G^G[ACGT]CC[CT]
582 AGGCCCC
EcoT22I 390 ATGCAT ATGCA^T
630 ATGCAT
HapII 58 CCGG C^CGG
101 CCGG
HgaI 118 GACGC GACGC(5/10)
130 GACGC
HhaI 49 GCGC GCG^C
73 GCGC
HinP1I 49 GCGC G^CGC
73 GCGC
HpaII 58 CCGG C^CGG
101 CCGG
MaeII 550 ACGT A^CGT
678 ACGT
MboI 163 GATC ^GATC
351 GATC
MflI 162 AGATCT [GA]^GATC[CT]
350 AGATCT
MseI 455 TTAA T^TAA
1111 TTAA
MspI 58 CCGG C^CGG
101 CCGG
NciI 57 CCCGG CC^[GC]GG
58 CCGGG
NdeII 163 GATC ^GATC
351 GATC
NruI 66 TCGCGA TCG^CGA
437 TCGCGA
NsiI 390 ATGCAT ATGCA^T
630 ATGCAT
PvuII 906 CAGCTG CAG^CTG
1143 CAGCTG
Sau3AI 163 GATC ^GATC
351 GATC
SmlI 300 CTCAAG C^T[CT][GA]AG
578 CTCAAG
SspBI 239 TGTACA T^GTACA
1084 TGTACA
TaqI 95 TCGA T^CGA
107 TCGA
Tru9I 455 TTAA T^TAA
1111 TTAA
TthHB8I 95 TCGA T^CGA
107 TCGA
XhoII 162 AGATCT [GA]^GATC[CT]
350 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BbvI BsaJI BsiYI BslI BsrI
BssKI BstF5I BstNI BstUI Cac8I
Cfr13I DdeI EarI EcoRII FauI
Fnu4HI FokI HaeIII HinfI ItaI
Ksp632I MaeIII MboII MnlI MslI
MspA1I MvaI MvnI MwoI NlaIII
NlaIV NspI PleI PstI RsaI
Sau96I ScrFI SfaNI SfcI TseI
Tsp509I TspRI