Restriction map for
pFN21AE6422
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AvrII BbeI
BbsI BclI BfaI BfrI BlnI
BpuAI BsaBI BsaHI BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII Bst1107I BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV FbaI FseI
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tru9I Tth111I XbaI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccII 796 CGCG CG^CG
ApaI 1439 GGGCCC GGGCC^C
Asp700I 774 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 925 CTCGAG C^[CT]CG[GA]G
AviII 563 TGCGCA TGC^GCA
BamHI 201 GGATCC G^GATCC
BbrPI 427 CACGTG CAC^GTG
BbvCI 196 GCTGAGG CCTCAGC(-5/-2)
BciVI 6 GGATAC GTATCC(6/5)
BglI 820 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 845 AGATCT A^GATCT
BlpI 990 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 990 GCTGAGC GC^T[ACGT]AGC
BsaI 959 GAGACC GGTCTC(1/5)
BsiEI 619 CGGCCG CG[GA][CT]^CG
BsmBI 1108 CGTCTC CGTCTC(1/5)
BsmI 248 GCATTC GAATGC(1/-1)
BsoBI 925 CTCGAG C^[CT]CG[GA]G
BsrFI 594 GCCGGT [GA]^CCGG[CT]
BssSI 971 CACGAG CACGAG(-5/-1)
BstAPI 1042 GCACCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 899 TTCGAA TT^CGAA
BstUI 796 CGCG CG^CG
CelII 990 GCTGAGC GC^T[ACGT]AGC
Cfr10I 594 GCCGGT [GA]^CCGG[CT]
DraIII 1043 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 913 GACCACCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 619 CGGCCG C^GGCCG
EclXI 619 CGGCCG C^GGCCG
Eco52I 619 CGGCCG C^GGCCG
EcoNI 1227 CCTGTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 406 ATGCAT ATGCA^T
FspI 563 TGCGCA TGC^GCA
HaeII 976 GGCGCT [GA]GCGC^[CT]
MslI 1186 CACCTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 796 CGCG CG^CG
NcoI 438 CCATGG C^CATGG
NsiI 406 ATGCAT ATGCA^T
NspV 899 TTCGAA TT^CGAA
PaeR7I 925 CTCGAG C^TCGAG
PflMI 1053 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 637 GACTC GAGTC(4/5)
PmaCI 427 CACGTG CAC^GTG
PmlI 427 CACGTG CAC^GTG
PpuMI 320 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 447 CAGCTG CAG^CTG
SacI 241 GAGCTC GAGCT^C
SfcI 103 CTACAG C^T[GA][CT]AG
SfuI 899 TTCGAA TT^CGAA
SgrAI 593 CGCCGGTG C[GA]^CCGG[CT]G
Van91I 1053 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 925 CTCGAG C^TCGAG
XmnI 774 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 762 GAATTT [GA]^AATT[CT]
1348 GAATTT
AfaI 738 GTAC GT^AC
1389 GTAC
Alw44I 968 GTGCAC G^TGCAC
1040 GTGCAC
ApaLI 968 GTGCAC G^TGCAC
1040 GTGCAC
ApoI 762 GAATTT [GA]^AATT[CT]
1348 GAATTT
BsaAI 427 CACGTG [CT]AC^GT[GA]
559 TACGTG
BsgI 1183 CTGCAC GTGCAG(16/14)
598 GTGCAG
BspMI 485 ACCTGC ACCTGC(4/8)
536 ACCTGC
BsrBI 544 CCGCTC CCGCTC(-3/-3)
469 GAGCGG
BsrDI 192 CATTGC GCAATG(2/0)
1196 GCAATG
BstYI 201 GGATCC [GA]^GATC[CT]
845 AGATCT
DsaI 438 CCATGG C^C[GA][CT]GG
963 CCGTGG
EarI 778 CTCTTC CTCTTC(1/4)
784 CTCTTC
EcoT14I 438 CCATGG C^C[AT][AT]GG
809 CCTTGG
FauI 1430 CCCGC CCCGC(4/6)
1437 GCGGG
HincII 673 GTTGAC GT[CT]^[GA]AC
1464 GTCAAC
HindII 673 GTTGAC GT[CT]^[GA]AC
1464 GTCAAC
Ksp632I 778 CTCTTC CTCTTC(1/4)
784 CTCTTC
MflI 201 GGATCC [GA]^GATC[CT]
845 AGATCT
RsaI 738 GTAC GT^AC
1389 GTAC
SexAI 731 ACCTGGT A^CC[AT]GGT
1031 ACCAGGT
SmlI 841 CTCAAG C^T[CT][GA]AG
925 CTCGAG
SphI 404 GCATGC GCATG^C
829 GCATGC
StyI 438 CCATGG C^C[AT][AT]GG
809 CCTTGG
TfiI 774 GAATC G^A[AT]TC
1446 GAATC
XhoII 201 GGATCC [GA]^GATC[CT]
845 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaII BalI BanI BanII BbvI
BcgI BcnI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI Sau3AI Sau96I ScrFI SfaNI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I