Restriction map for
pFN21AE2245
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BclI BfrI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmFI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HaeIII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MamI MluI MluNI
MroI MscI MspA1I MvnI NaeI
NarI NgoMIV NheI NlaIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AlwI 354 GGATC GGATC(4/5)
AvrII 222 CCTAGG C^CTAGG
BciVI 252 GGATAC GTATCC(6/5)
BcnI 35 CCCGG CC^[GC]GG
BfaI 223 CTAG C^TAG
BlnI 222 CCTAGG C^CTAGG
BsgI 169 GTGCAG GTGCAG(16/14)
BsmI 28 GCATTC GAATGC(1/-1)
BspHI 243 TCATGA T^CATGA
BspMI 188 ACCTGC ACCTGC(4/8)
BsrDI 54 GCAATG GCAATG(2/0)
BstF5I 372 CATCC GGATG(2/0)
BstYI 354 GGATCT [GA]^GATC[CT]
Cac8I 454 GCAGGC GC[ACGT]^[ACGT]GC
DpnII 355 GATC ^GATC
DraII 173 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 211 CCATGG C^C[GA][CT]GG
EcoNI 9 CCTTAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 173 AGGACCT [GA]G^G[ACGT]CC[CT]
FokI 372 CATCC GGATG(9/13)
HapII 36 CCGG C^CGG
HinfI 276 GACTC G^A[ACGT]TC
HpaII 36 CCGG C^CGG
MaeI 223 CTAG C^TAG
MboI 355 GATC ^GATC
MfeI 476 CAATTG C^AATTG
MflI 354 GGATCT [GA]^GATC[CT]
MspI 36 CCGG C^CGG
MunI 476 CAATTG C^AATTG
NciI 35 CCCGG CC^[GC]GG
NcoI 211 CCATGG C^CATGG
NdeII 355 GATC ^GATC
PflMI 565 CCAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 276 GACTC GAGTC(4/5)
PpuMI 173 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 243 TCATGA T^CATGA
Sau3AI 355 GATC ^GATC
Van91I 565 CCAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 354 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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AfaI 3 GTAC GT^AC
593 GTAC
AluI 181 AGCT AG^CT
587 AGCT
AvaII 174 GGACC G^G[AT]CC
186 GGACC
BsrI 79 ACTGG ACTGG(1/-1)
521 CCAGT
Cfr13I 174 GGACC G^G[ACGT]CC
186 GGACC
EcoRI 116 GAATTC G^AATTC
498 GAATTC
EcoT14I 211 CCATGG C^C[AT][AT]GG
222 CCTAGG
MboII 130 GAAGA GAAGA(8/7)
409 GAAGA
MslI 60 CACCAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
445 CATTGTAATG
MwoI 210 GCCATGGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
608 GCAAAGAAAGC
NdeI 296 CATATG CA^TATG
558 CATATG
RsaI 3 GTAC GT^AC
593 GTAC
Sau96I 174 GGACC G^G[ACGT]CC
186 GGACC
SfaNI 109 GATGC GCATC(5/9)
235 GATGC
StyI 211 CCATGG C^C[AT][AT]GG
222 CCTAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AcsI ApoI BbvI BsaJI BsiYI
BslI BssKI BstNI DdeI EcoRII
Fnu4HI HphI ItaI MaeIII MnlI
MseI MvaI NlaIII ScrFI Tru9I
TseI Tsp45I Tsp509I TspRI