Restriction map for
pFN21AE3922
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AocI
ApaI AscI Asp718I AspEI AspI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BciVI BfrI
BglI BlnI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspHI BspMI
BsrBI BssSI Bst1107I BstEII BstPI
BstXI BstZ17I Bsu36I ClaI CpoI
DrdI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
FauI FseI FspI Hin1I HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Alw44I 1633 GTGCAC G^TGCAC
AlwNI 1232 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 361 AGCGCT AGC^GCT
ApaLI 1633 GTGCAC G^TGCAC
Asp700I 1409 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1633 GTGCAC G[AT]GC[AT]^C
BanI 2160 GGTGCC G^G[CT][GA]CC
BcgI 2063 CGAAAAATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1405 CCGGG CC^[GC]GG
BglII 1937 AGATCT A^GATCT
BlpI 918 GCTTAGC GC^T[ACGT]AGC
BmyI 1633 GTGCAC G[AGT]GC[ACT]^C
Bpu1102I 918 GCTTAGC GC^T[ACGT]AGC
BsiHKAI 1633 GTGCAC G[AT]GC[AT]^C
BsmFI 2521 GTCCC GGGAC(10/14)
Bsp1286I 1633 GTGCAC G[AGT]GC[ACT]^C
BsrFI 1349 ACCGGC [GA]^CCGG[CT]
BssHII 52 GCGCGC G^CGCGC
CelII 918 GCTTAGC GC^T[ACGT]AGC
Cfr10I 1349 ACCGGC [GA]^CCGG[CT]
DraIII 2525 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 361 AGCGCT AGC^GCT
EcoRV 1657 GATATC GAT^ATC
HaeII 361 AGCGCT [GA]GCGC^[CT]
HgaI 33 GACGC GACGC(5/10)
HincII 370 GTCAAC GT[CT]^[GA]AC
HindII 370 GTCAAC GT[CT]^[GA]AC
NciI 1405 CCGGG CC^[GC]GG
NdeI 2293 CATATG CA^TATG
PleI 1150 GACTC GAGTC(4/5)
SapI 951 GAAGAGC GCTCTTC(1/4)
SphI 2360 GCATGC GCATG^C
SspI 1975 AATATT AAT^ATT
StuI 390 AGGCCT AGG^CCT
XbaI 2109 TCTAGA T^CTAGA
XmnI 1409 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AseI 99 ATTAAT AT^TAAT
741 ATTAAT
AsnI 99 ATTAAT AT^TAAT
741 ATTAAT
AvaI 70 CCCGAG C^[CT]CG[GA]G
756 CCCGAG
BbsI 796 GAAGAC GAAGAC(2/6)
2461 GAAGAC
BclI 120 TGATCA T^GATCA
1905 TGATCA
BpmI 1229 CTCCAG CTGGAG(16/14)
829 CTGGAG
BpuAI 796 GAAGAC GAAGAC(2/6)
2461 GAAGAC
BsoBI 70 CCCGAG C^[CT]CG[GA]G
756 CCCGAG
Bsp1407I 295 TGTACA T^GTACA
708 TGTACA
BspLU11I 812 ACATGT A^CATGT
1379 ACATGT
BsrGI 295 TGTACA T^GTACA
708 TGTACA
BstAPI 540 GCAGTGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1717 GCAAATTGTGC
BstBI 992 TTCGAA TT^CGAA
2240 TTCGAA
DraI 1458 TTTAAA TTT^AAA
1570 TTTAAA
DraII 1233 AGGACCT [GA]G^G[ACGT]CC[CT]
2519 GGGTCCC
EarI 951 GAAGAG CTCTTC(1/4)
2155 GAAGAG
EcoO109I 1233 AGGACCT [GA]G^G[ACGT]CC[CT]
2519 GGGTCCC
EcoT14I 485 CCATGG C^C[AT][AT]GG
1758 CCATGG
FbaI 120 TGATCA T^GATCA
1905 TGATCA
HapII 1350 CCGG C^CGG
1405 CCGG
HindIII 198 AAGCTT A^AGCTT
1370 AAGCTT
HpaII 1350 CCGG C^CGG
1405 CCGG
HphI 753 TCACC GGTGA(8/7)
1417 TCACC
Ksp632I 951 GAAGAG CTCTTC(1/4)
2155 GAAGAG
MslI 457 CATGTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2628 CATCAACATG
MspA1I 1856 CAGCTG C[AC]G^C[GT]G
1966 CAGCTG
MspI 1350 CCGG C^CGG
1405 CCGG
NcoI 485 CCATGG C^CATGG
1758 CCATGG
NspV 992 TTCGAA TT^CGAA
2240 TTCGAA
PpuMI 1233 AGGACCT [GA]G^G[AT]CC[CT]
2519 GGGTCCC
PshBI 99 ATTAAT AT^TAAT
741 ATTAAT
PvuII 1856 CAGCTG CAG^CTG
1966 CAGCTG
SfuI 992 TTCGAA TT^CGAA
2240 TTCGAA
SspBI 295 TGTACA T^GTACA
708 TGTACA
StyI 485 CCATGG C^C[AT][AT]GG
1758 CCATGG
TaqI 993 TCGA T^CGA
2241 TCGA
TthHB8I 993 TCGA T^CGA
2241 TCGA
XcmI 1851 CCAGTCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2431 CCAGTTATTCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AvaII BbvI
BfaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EcoRII EcoT22I Fnu4HI FokI
HaeIII HhaI HinP1I HinfI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MvaI MvnI
MwoI NdeII NlaIII NlaIV NsiI
NspI PflMI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XhoII