Restriction map for
pFN21AE5618
##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AccI AccII AccIII AciI AclI
AflII AgeI AhdI AocI Aor51HI
ApaI AscI AspEI AviII AvrII
BamHI BanII BbeI BbrPI BbsI
BbvCI BclI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BspHI BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HpaI KasI KspI MamI
MluI MroI MslI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspI StuI SwaI
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AatII 6 GACGTC GACGT^C
Acc65I 999 GGTACC G^GTACC
AcyI 6 GACGTC G[GA]^CG[CT]C
AflIII 1588 ACATGT A^C[GA][CT]GT
Alw44I 1067 GTGCAC G^TGCAC
AlwNI 245 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1067 GTGCAC G^TGCAC
AseI 1074 ATTAAT AT^TAAT
AsnI 1074 ATTAAT AT^TAAT
Asp700I 839 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 999 GGTACC G^GTACC
AspI 1239 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1388 CTCGAG C^[CT]CG[GA]G
AvaII 22 GGACC G^G[AT]CC
BalI 1374 TGGCCA TGG^CCA
BanI 999 GGTACC G^G[CT][GA]CC
BcgI 5 CGACGTCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 288 GGATAC GTATCC(6/5)
BcnI 51 CCGGG CC^[GC]GG
BglII 465 AGATCT A^GATCT
BsaAI 1519 CACGTA [CT]AC^GT[GA]
BsaHI 6 GACGTC G[GA]^CG[CT]C
BsgI 1400 GTGCAG GTGCAG(16/14)
BsmBI 8 CGTCTC CGTCTC(1/5)
BsoBI 1388 CTCGAG C^[CT]CG[GA]G
Bsp1407I 91 TGTACA T^GTACA
BspLU11I 1588 ACATGT A^CATGT
BsrDI 991 GCAATG GCAATG(2/0)
BsrGI 91 TGTACA T^GTACA
BsrI 1255 ACTGG ACTGG(1/-1)
CfoI 1537 GCGC GCG^C
DraII 21 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1519 CACGTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 271 GAAGAG CTCTTC(1/4)
EcoO109I 21 GGGACCC [GA]G^G[ACGT]CC[CT]
HapII 51 CCGG C^CGG
HhaI 1537 GCGC GCG^C
Hin1I 6 GACGTC G[GA]^CG[CT]C
HinP1I 1537 GCGC G^CGC
HindIII 562 AAGCTT A^AGCTT
HpaII 51 CCGG C^CGG
KpnI 999 GGTACC GGTAC^C
Ksp632I 271 GAAGAG CTCTTC(1/4)
MfeI 104 CAATTG C^AATTG
MluNI 1374 TGGCCA TGG^CCA
MscI 1374 TGGCCA TGG^CCA
MspA1I 34 CAGCTG C[AC]G^C[GT]G
MspI 51 CCGG C^CGG
MunI 104 CAATTG C^AATTG
NciI 51 CCGGG CC^[GC]GG
NdeI 130 CATATG CA^TATG
NspI 1588 ACATGT [GA]CATG^[CT]
PaeR7I 1388 CTCGAG C^TCGAG
PflFI 1239 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 21 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 1074 ATTAAT AT^TAAT
PvuII 34 CAGCTG CAG^CTG
SspBI 91 TGTACA T^GTACA
TaqI 1389 TCGA T^CGA
Tth111I 1239 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1389 TCGA T^CGA
XcmI 26 CCACGAACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1388 CTCGAG C^TCGAG
XmnI 839 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 39 GGATC GGATC(4/5)
1258 GGATC
AspHI 1067 GTGCAC G[AT]GC[AT]^C
1301 GAGCAC
BpmI 203 CTCCAG CTGGAG(16/14)
864 CTCCAG
BsaI 389 GGTCTC GGTCTC(1/5)
932 GGTCTC
BseRI 1382 CTCCTC GAGGAG(10/8)
1385 CTCCTC
BsiHKAI 1067 GTGCAC G[AT]GC[AT]^C
1301 GAGCAC
BstYI 39 GGATCT [GA]^GATC[CT]
465 AGATCT
EaeI 348 TGGCCG [CT]^GGCC[GA]
1374 TGGCCA
EcoNI 146 CCTGCTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
394 CCTGAGTTAGG
EcoRI 208 GAATTC G^AATTC
644 GAATTC
HincII 264 GTCAAC GT[CT]^[GA]AC
1229 GTCAAC
HindII 264 GTCAAC GT[CT]^[GA]AC
1229 GTCAAC
MaeII 7 ACGT A^CGT
1520 ACGT
MflI 39 GGATCT [GA]^GATC[CT]
465 AGATCT
PflMI 1492 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1518 CCACGTAGTGG
PstI 797 CTGCAG CTGCA^G
1234 CTGCAG
SmlI 150 CTTGAG C^T[CT][GA]AG
1388 CTCGAG
SpeI 1087 ACTAGT A^CTAGT
1581 ACTAGT
Van91I 1492 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1518 CCACGTAGTGG
XhoII 39 GGATCT [GA]^GATC[CT]
465 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BfaI BmyI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DsaI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MvaI MwoI NcoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI