Restriction map for
pFN21AE9529
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI AocI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstBI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraI DrdI
DsaI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MluI MroI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuII
RcaI RsaI RsrII SacII SalI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SwaI TaqI
Tth111I TthHB8I XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AcsI 817 GAATTC [GA]^AATT[CT]
Alw44I 418 GTGCAC G^TGCAC
AlwNI 239 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 747 AGCGCT AGC^GCT
ApaI 788 GGGCCC GGGCC^C
ApaLI 418 GTGCAC G^TGCAC
ApoI 817 GAATTC [GA]^AATT[CT]
AvaI 461 CCCGGG C^[CT]CG[GA]G
AvrII 156 CCTAGG C^CTAGG
BglII 546 AGATCT A^GATCT
BlnI 156 CCTAGG C^CTAGG
BpmI 86 CTCCAG CTGGAG(16/14)
BsgI 106 CTGCAC GTGCAG(16/14)
BsiEI 650 CGATCG CG[GA][CT]^CG
BsoBI 461 CCCGGG C^[CT]CG[GA]G
BsrFI 947 GCCGGC [GA]^CCGG[CT]
BssSI 763 CTCGTG CACGAG(-5/-1)
BstAPI 108 GCACAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 465 GGTCACC G^GT[ACGT]ACC
BstPI 465 GGTCACC G^GT[ACGT]ACC
BstXI 88 CCAGAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 546 AGATCT [GA]^GATC[CT]
CfoI 748 GCGC GCG^C
Cfr10I 947 GCCGGC [GA]^CCGG[CT]
DraIII 109 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 747 AGCGCT AGC^GCT
EcoO65I 465 GGTCACC G^GT[ACGT]ACC
EcoRI 817 GAATTC G^AATTC
HaeII 747 AGCGCT [GA]GCGC^[CT]
HhaI 748 GCGC GCG^C
HinP1I 748 GCGC G^CGC
HinfI 921 GAATC G^A[ACGT]TC
MaeII 209 ACGT A^CGT
MfeI 883 CAATTG C^AATTG
MflI 546 AGATCT [GA]^GATC[CT]
MspA1I 445 CAGCGG C[AC]G^C[GT]G
MunI 883 CAATTG C^AATTG
NaeI 947 GCCGGC GCC^GGC
NgoMIV 947 GCCGGC G^CCGGC
NlaIII 583 CATG CATG^
PpuMI 98 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 960 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 650 CGATCG CGAT^CG
SacI 25 GAGCTC GAGCT^C
SmaI 461 CCCGGG CCC^GGG
SmlI 501 CTTGAG C^T[CT][GA]AG
SspI 619 AATATT AAT^ATT
StuI 587 AGGCCT AGG^CCT
TfiI 921 GAATC G^A[AT]TC
TspRI 641 CACTG CA[GC]TG[ACGT][ACGT]^
XbaI 21 TCTAGA T^CTAGA
XhoII 546 AGATCT [GA]^GATC[CT]
XmaCI 461 CCCGGG C^CCGGG
XmaI 461 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 409 CGCG CG^CG
692 CGCG
AlwI 527 GATCC GGATC(4/5)
753 GATCC
BbsI 181 GTCTTC GAAGAC(2/6)
655 GTCTTC
BfaI 22 CTAG C^TAG
157 CTAG
BglI 192 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
229 GCCAACCTGGC
BpuAI 181 GTCTTC GAAGAC(2/6)
655 GTCTTC
BsmFI 98 GGGAC GGGAC(10/14)
541 GTCCC
BspMI 101 ACCTGC ACCTGC(4/8)
469 ACCTGC
BstUI 409 CGCG CG^CG
692 CGCG
EarI 52 CTCTTC CTCTTC(1/4)
952 CTCTTC
EcoT14I 156 CCTAGG C^C[AT][AT]GG
962 CCAAGG
FauI 693 GCGGG CCCGC(4/6)
719 GCGGG
HphI 467 TCACC GGTGA(8/7)
557 TCACC
Ksp632I 52 CTCTTC CTCTTC(1/4)
952 CTCTTC
MaeI 22 CTAG C^TAG
157 CTAG
MaeIII 77 GTGAC ^GT[ACGT]AC
466 GTCAC
MseI 302 TTAA T^TAA
616 TTAA
MslI 468 CACCTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
759 CACGCTCGTG
MvnI 409 CGCG CG^CG
692 CGCG
PflMI 669 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
962 CCAAGGTCTGG
SapI 51 GCTCTTC GCTCTTC(1/4)
951 GCTCTTC
StyI 156 CCTAGG C^C[AT][AT]GG
962 CCAAGG
Tru9I 302 TTAA T^TAA
616 TTAA
Tsp45I 77 GTGAC ^GT[GC]AC
466 GTCAC
Van91I 669 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
962 CCAAGGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AspHI AvaII BalI
BanII BbvI BcnI BmyI BsaJI
BseRI BsiHKAI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DpnII DraII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HpaII ItaI MboI MboII MluNI
MnlI MscI MspI MvaI MwoI
NciI NdeII NlaIV Sau3AI Sau96I
ScrFI SfaNI TseI Tsp509I