Restriction map for
pFN21AE4582
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII AccIII AcyI AflII AflIII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I AspEI
AvrII BamHI BbeI BcgI BfrI
BglI BlnI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI DraI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI HaeII Hin1I HincII
HindII HindIII HpaI KasI MfeI
MluI MroI MunI NarI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PshBI PstI
PvuI RcaI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI XbaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 1876 GGTACC G^GTACC
AccI 1453 GTCTAC GT^[AC][GT]AC
AclI 2218 AACGTT AA^CGTT
AcsI 821 GAATTT [GA]^AATT[CT]
Alw44I 2332 GTGCAC G^TGCAC
ApaLI 2332 GTGCAC G^TGCAC
ApoI 821 GAATTT [GA]^AATT[CT]
Asp718I 1876 GGTACC G^GTACC
AspI 949 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2063 TGCGCA TGC^GCA
BbrPI 538 CACGTG CAC^GTG
BbvCI 1486 GCTGAGG CCTCAGC(-5/-2)
BciVI 271 GTATCC GTATCC(6/5)
BglII 725 AGATCT A^GATCT
BsgI 1834 CTGCAC GTGCAG(16/14)
BstAPI 1403 GCATCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 1928 CGGACCG CG^G[AT]CCG
DraIII 883 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1000 GACAATGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 2063 TGCGCA TGC^GCA
KpnI 1876 GGTACC GGTAC^C
KspI 426 CCGCGG CCGC^GG
MseI 806 TTAA T^TAA
NaeI 263 GCCGGC GCC^GGC
NgoMIV 263 GCCGGC G^CCGGC
PaeR7I 2438 CTCGAG C^TCGAG
PflFI 949 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 538 CACGTG CAC^GTG
PmlI 538 CACGTG CAC^GTG
PpuMI 183 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 2218 AACGTT AA^CGTT
PvuII 296 CAGCTG CAG^CTG
RsrII 1928 CGGACCG CG^G[AT]CCG
SacI 354 GAGCTC GAGCT^C
SacII 426 CCGCGG CCGC^GG
SphI 323 GCATGC GCATG^C
Tru9I 806 TTAA T^TAA
Tsp509I 822 AATT ^AATT
Tth111I 949 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1895 CCAAGAAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2438 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BclI 542 TGATCA T^GATCA
1799 TGATCA
BfaI 328 CTAG C^TAG
1394 CTAG
BsaAI 538 CACGTG [CT]AC^GT[GA]
769 TACGTG
BsaBI 726 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1794 GATGCTGATC
EarI 736 CTCTTC CTCTTC(1/4)
1329 GAAGAG
EcoT14I 980 CCATGG C^C[AT][AT]GG
1235 CCATGG
FbaI 542 TGATCA T^GATCA
1799 TGATCA
Ksp632I 736 CTCTTC CTCTTC(1/4)
1329 GAAGAG
MaeI 328 CTAG C^TAG
1394 CTAG
MamI 726 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1794 GATGCTGATC
NcoI 980 CCATGG C^CATGG
1235 CCATGG
NdeI 442 CATATG CA^TATG
932 CATATG
NspI 323 GCATGC [GA]CATG^[CT]
1604 ACATGC
PflMI 1971 CCATCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2362 CCAGCCCCTGG
SmlI 493 CTTGAG C^T[CT][GA]AG
2438 CTCGAG
StyI 980 CCATGG C^C[AT][AT]GG
1235 CCATGG
Van91I 1971 CCATCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2362 CCAGCCCCTGG
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# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmaI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I XhoII XmaCI XmaI