Restriction map for
pFN21AB6122
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsiEI
BsiWI BspHI BspLU11I BspMI BsrDI
BsrFI BssHII Bst1107I BstAPI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MscI MslI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccII 1390 CGCG CG^CG
AccIII 1375 TCCGGA T^CCGGA
AfaI 1114 GTAC GT^AC
AlwI 1317 GATCC GGATC(4/5)
AlwNI 451 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 180 GAGCTC G[AT]GC[AT]^C
AvaI 927 CCCGGG C^[CT]CG[GA]G
BbsI 1728 GTCTTC GAAGAC(2/6)
BcgI 1254 GCAACAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1666 GGATAC GTATCC(6/5)
BpuAI 1728 GTCTTC GAAGAC(2/6)
BsaWI 1375 TCCGGA [AT]^CCGG[AT]
BseAI 1375 TCCGGA T^CCGGA
BsgI 1204 GTGCAG GTGCAG(16/14)
BsiHKAI 180 GAGCTC G[AT]GC[AT]^C
BsmAI 1104 GAGAC GTCTC(1/5)
BsmBI 1104 GAGACG CGTCTC(1/5)
BsmI 918 GAATGC GAATGC(1/-1)
BsoBI 927 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1113 TGTACA T^GTACA
BspDI 607 ATCGAT AT^CGAT
BspEI 1375 TCCGGA T^CCGGA
BsrBI 1235 CCGCTC CCGCTC(-3/-3)
BsrGI 1113 TGTACA T^GTACA
BstBI 881 TTCGAA TT^CGAA
BstUI 1390 CGCG CG^CG
BstYI 1316 AGATCC [GA]^GATC[CT]
CfoI 732 GCGC GCG^C
ClaI 607 ATCGAT AT^CGAT
EcoNI 789 CCTGACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 41 CCCGC CCCGC(4/6)
HgaI 1388 GACGC GACGC(5/10)
HhaI 732 GCGC GCG^C
HinP1I 732 GCGC G^CGC
HphI 898 GGTGA GGTGA(8/7)
MaeII 1107 ACGT A^CGT
MflI 1316 AGATCC [GA]^GATC[CT]
MroI 1375 TCCGGA T^CCGGA
MvnI 1390 CGCG CG^CG
NspV 881 TTCGAA TT^CGAA
PflMI 735 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 689 CAGCTG CAG^CTG
RsaI 1114 GTAC GT^AC
SacI 180 GAGCTC GAGCT^C
SapI 832 GAAGAGC GCTCTTC(1/4)
SfuI 881 TTCGAA TT^CGAA
SmaI 927 CCCGGG CCC^GGG
SmlI 18 CTCAAG C^T[CT][GA]AG
SspBI 1113 TGTACA T^GTACA
Tsp45I 581 GTGAC ^GT[GC]AC
Van91I 735 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 794 CCAAGGCTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1316 AGATCC [GA]^GATC[CT]
XmaCI 927 CCCGGG C^CCGGG
XmaI 927 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AcsI 104 GAATTT [GA]^AATT[CT]
1334 AAATTC
ApoI 104 GAATTT [GA]^AATT[CT]
1334 AAATTC
AvaII 1715 GGTCC G^G[AT]CC
1741 GGACC
BfaI 110 CTAG C^TAG
1357 CTAG
BsmFI 1740 GGGAC GGGAC(10/14)
1140 GTCCC
BssSI 859 CACGAG CACGAG(-5/-1)
578 CTCGTG
EaeI 1467 CGGCCA [CT]^GGCC[GA]
1560 CGGCCA
EcoRV 1071 GATATC GAT^ATC
1230 GATATC
MaeI 110 CTAG C^TAG
1357 CTAG
MaeIII 442 GTAAC ^GT[ACGT]AC
581 GTGAC
MseI 617 TTAA T^TAA
1313 TTAA
PleI 942 GACTC GAGTC(4/5)
1726 GAGTC
SfaNI 810 GATGC GCATC(5/9)
1003 GATGC
Tru9I 617 TTAA T^TAA
1313 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI BanII BbvI BcnI
BglI BmyI BpmI BsaJI BseRI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DsaI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI Ksp632I MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfcI StyI TaqI
TfiI TseI Tsp509I TspRI TthHB8I